Organization of the gene encoding transcriptional repressor deltaEF1 and cross-species conservation of its domains

R Sekido, T Takagi, M Okanami, H Moribe, M Yamamura, Y Higashi, H Kondoh

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

DeltaEF1 (delta-crystallin/E2-box factor 1) is a widely distributed repressor of transcription which binds at the E2-box sequence, CACCTG. It carries seven zinc fingers (Zf) in two clusters and a homeodomain in the middle as potential DNA-binding domains. We cloned the genomic gene encoding chicken deltaEF1 and analyzed its organization. The gene consisted of nine exons, the N-proximal Zf were encoded by exons 5 through 7, and the C-proximal Zf by exons 8 and 9. Exon 7 also coded for the large middle portion of the protein including the homeodomain. Promoter analysis and RNase-protection assay indicated that the gene is driven by a G+C-rich promoter without a TATA box, and the transcription start points (tsp) cluster around 20 bp from the start codon located in exon 1. cDNA and genomic sequences of the mouse delta EF1 were cloned and compared with the chicken sequence. The deduced amino acid (aa) sequence was highly conserved between the chicken and mouse deltaEF1, no only in DNA-binding motifs but also in other blocks (78% overall aa identity). More recently reported DNA-binding proteins, AREB6 (human) ZEB (human) and BZP (hamster), were attributed to homologues of deltaEF1, among which only AREB6 represented full-length sequence. It was also indicated that rodent deltaEF1 lacked exon 3.

Original languageEnglish
Pages (from-to)227-32
Number of pages6
JournalGene
Volume173
Issue number2
Publication statusPublished - 16 Sep 1996

Fingerprint

Exons
Zinc Fingers
Genes
Chickens
delta-Crystallins
Homeodomain Proteins
TATA Box
Nucleotide Motifs
Initiator Codon
DNA-Binding Proteins
Ribonucleases
Cricetinae
Amino Acid Sequence
Rodentia
Complementary DNA
Amino Acids
DNA

Keywords

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Binding Sites
  • Chickens
  • Cloning, Molecular
  • Conserved Sequence
  • Crystallins
  • DNA
  • DNA-Binding Proteins
  • Homeodomain Proteins
  • Humans
  • Mice
  • Molecular Sequence Data
  • Nuclear Proteins
  • Promoter Regions, Genetic
  • Repressor Proteins
  • Restriction Mapping
  • Sequence Homology, Amino Acid
  • Species Specificity
  • Transcription Factors

Cite this

Sekido, R., Takagi, T., Okanami, M., Moribe, H., Yamamura, M., Higashi, Y., & Kondoh, H. (1996). Organization of the gene encoding transcriptional repressor deltaEF1 and cross-species conservation of its domains. Gene, 173(2), 227-32.

Organization of the gene encoding transcriptional repressor deltaEF1 and cross-species conservation of its domains. / Sekido, R; Takagi, T; Okanami, M; Moribe, H; Yamamura, M; Higashi, Y; Kondoh, H.

In: Gene, Vol. 173, No. 2, 16.09.1996, p. 227-32.

Research output: Contribution to journalArticle

Sekido, R, Takagi, T, Okanami, M, Moribe, H, Yamamura, M, Higashi, Y & Kondoh, H 1996, 'Organization of the gene encoding transcriptional repressor deltaEF1 and cross-species conservation of its domains', Gene, vol. 173, no. 2, pp. 227-32.
Sekido R, Takagi T, Okanami M, Moribe H, Yamamura M, Higashi Y et al. Organization of the gene encoding transcriptional repressor deltaEF1 and cross-species conservation of its domains. Gene. 1996 Sep 16;173(2):227-32.
Sekido, R ; Takagi, T ; Okanami, M ; Moribe, H ; Yamamura, M ; Higashi, Y ; Kondoh, H. / Organization of the gene encoding transcriptional repressor deltaEF1 and cross-species conservation of its domains. In: Gene. 1996 ; Vol. 173, No. 2. pp. 227-32.
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N2 - DeltaEF1 (delta-crystallin/E2-box factor 1) is a widely distributed repressor of transcription which binds at the E2-box sequence, CACCTG. It carries seven zinc fingers (Zf) in two clusters and a homeodomain in the middle as potential DNA-binding domains. We cloned the genomic gene encoding chicken deltaEF1 and analyzed its organization. The gene consisted of nine exons, the N-proximal Zf were encoded by exons 5 through 7, and the C-proximal Zf by exons 8 and 9. Exon 7 also coded for the large middle portion of the protein including the homeodomain. Promoter analysis and RNase-protection assay indicated that the gene is driven by a G+C-rich promoter without a TATA box, and the transcription start points (tsp) cluster around 20 bp from the start codon located in exon 1. cDNA and genomic sequences of the mouse delta EF1 were cloned and compared with the chicken sequence. The deduced amino acid (aa) sequence was highly conserved between the chicken and mouse deltaEF1, no only in DNA-binding motifs but also in other blocks (78% overall aa identity). More recently reported DNA-binding proteins, AREB6 (human) ZEB (human) and BZP (hamster), were attributed to homologues of deltaEF1, among which only AREB6 represented full-length sequence. It was also indicated that rodent deltaEF1 lacked exon 3.

AB - DeltaEF1 (delta-crystallin/E2-box factor 1) is a widely distributed repressor of transcription which binds at the E2-box sequence, CACCTG. It carries seven zinc fingers (Zf) in two clusters and a homeodomain in the middle as potential DNA-binding domains. We cloned the genomic gene encoding chicken deltaEF1 and analyzed its organization. The gene consisted of nine exons, the N-proximal Zf were encoded by exons 5 through 7, and the C-proximal Zf by exons 8 and 9. Exon 7 also coded for the large middle portion of the protein including the homeodomain. Promoter analysis and RNase-protection assay indicated that the gene is driven by a G+C-rich promoter without a TATA box, and the transcription start points (tsp) cluster around 20 bp from the start codon located in exon 1. cDNA and genomic sequences of the mouse delta EF1 were cloned and compared with the chicken sequence. The deduced amino acid (aa) sequence was highly conserved between the chicken and mouse deltaEF1, no only in DNA-binding motifs but also in other blocks (78% overall aa identity). More recently reported DNA-binding proteins, AREB6 (human) ZEB (human) and BZP (hamster), were attributed to homologues of deltaEF1, among which only AREB6 represented full-length sequence. It was also indicated that rodent deltaEF1 lacked exon 3.

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