Oriented attachment of VNAR proteins, Q2 via site-selective modification, on PLGA–PEG nanoparticles enhances nanoconjugate performance

Joao C. F. Nogueira; Michelle K. Greene; Daniel A. Richards; Alexander O. Furby; John Steven; Andrew Porter; Caroline Barelle; Christopher J. Scott; Vijay Chudasama

doi:10.1039/c9cc02655j

Oriented attachment of VNAR proteins, Q2 via site-selective modification, on PLGA–PEG nanoparticles enhances nanoconjugate performance

Joao C. F. Nogueira, Michelle K. Greene, Daniel A. Richards, Alexander O. Furby, John Steven, Andrew Porter, Caroline Barelle^* (Corresponding Author), Christopher J. Scott^* (Corresponding Author), Vijay Chudasama^* (Corresponding Author)

^*Corresponding author for this work

Medical Sciences

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

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Abstract

Herein we report the construction of a nanoparticle-based drug delivery system which targets a key regulator in tumour angiogenesis. We exploit a Variable New Antigen Receptor (VNAR) domain, conjugated using site-specific chemistry, to direct poly lactic acidco-glycolic acid–polyethylene glycol (PLGA–PEG) nanoparticles to delta like canonical Notch ligand 4 (DLL4). The importance of sitespecific chemistry is demonstrated.

Original language	English
Pages (from-to)	7671-7674
Number of pages	4
Journal	Chemical Communications
Volume	55
Issue number	53
Early online date	29 May 2019
DOIs	https://doi.org/10.1039/c9cc02655j
Publication status	Published - 7 Jul 2019

Bibliographical note

This work was partially funded through a US-Ireland R&D Partnership grant (STL/5010/14, MRC grant MC_PC_15013). JCFN is funded by the EU’s Horizon 2020 programme under Marie-Curie grant agreement 675007. We acknowledge UCL
Chemistry Mass Spectrometry Facility (Dr K. Karu/Dr X. Yang).

Keywords

MONOCLONAL-ANTIBODY
TARGETED DELIVERY
FRAGMENTS
OPTIMIZATION
INSTALLATION
CONJUGATION
DRUGS

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10.1039/c9cc02655jLicence: CC BY

Oriented attachment of VNAR proteins, via site-selective modification, on PLGA–PEG nanoparticles enhances nanoconjugate performance
© The Royal Society of Chemistry 2019. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence https://creativecommons.org/licenses/by/3.0/
Final published version, 1.99 MBLicence: CC BY
Supplementary Information
This article is licensed under a Creative Commons Attribution 3.0 Unported Licence https://creativecommons.org/licenses/by/3.0/
Supplementary Data, 1.95 MBLicence: CC BY

Cite this

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title = "Oriented attachment of VNAR proteins, Q2 via site-selective modification, on PLGA–PEG nanoparticles enhances nanoconjugate performance",

abstract = "Herein we report the construction of a nanoparticle-based drug delivery system which targets a key regulator in tumour angiogenesis. We exploit a Variable New Antigen Receptor (VNAR) domain, conjugated using site-specific chemistry, to direct poly lactic acidco-glycolic acid–polyethylene glycol (PLGA–PEG) nanoparticles to delta like canonical Notch ligand 4 (DLL4). The importance of sitespecific chemistry is demonstrated.",

keywords = "MONOCLONAL-ANTIBODY, TARGETED DELIVERY, FRAGMENTS, OPTIMIZATION, INSTALLATION, CONJUGATION, DRUGS",

author = "Nogueira, {Joao C. F.} and Greene, {Michelle K.} and Richards, {Daniel A.} and Furby, {Alexander O.} and John Steven and Andrew Porter and Caroline Barelle and Scott, {Christopher J.} and Vijay Chudasama",

note = "This work was partially funded through a US-Ireland R&D Partnership grant (STL/5010/14, MRC grant MC_PC_15013). JCFN is funded by the EU{\textquoteright}s Horizon 2020 programme under Marie-Curie grant agreement 675007. We acknowledge UCL Chemistry Mass Spectrometry Facility (Dr K. Karu/Dr X. Yang).",

year = "2019",

month = jul,

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doi = "10.1039/c9cc02655j",

language = "English",

volume = "55",

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AU - Nogueira, Joao C. F.

AU - Greene, Michelle K.

AU - Richards, Daniel A.

AU - Furby, Alexander O.

AU - Steven, John

AU - Porter, Andrew

AU - Barelle, Caroline

AU - Scott, Christopher J.

AU - Chudasama, Vijay

N1 - This work was partially funded through a US-Ireland R&D Partnership grant (STL/5010/14, MRC grant MC_PC_15013). JCFN is funded by the EU’s Horizon 2020 programme under Marie-Curie grant agreement 675007. We acknowledge UCL Chemistry Mass Spectrometry Facility (Dr K. Karu/Dr X. Yang).

PY - 2019/7/7

Y1 - 2019/7/7

N2 - Herein we report the construction of a nanoparticle-based drug delivery system which targets a key regulator in tumour angiogenesis. We exploit a Variable New Antigen Receptor (VNAR) domain, conjugated using site-specific chemistry, to direct poly lactic acidco-glycolic acid–polyethylene glycol (PLGA–PEG) nanoparticles to delta like canonical Notch ligand 4 (DLL4). The importance of sitespecific chemistry is demonstrated.

AB - Herein we report the construction of a nanoparticle-based drug delivery system which targets a key regulator in tumour angiogenesis. We exploit a Variable New Antigen Receptor (VNAR) domain, conjugated using site-specific chemistry, to direct poly lactic acidco-glycolic acid–polyethylene glycol (PLGA–PEG) nanoparticles to delta like canonical Notch ligand 4 (DLL4). The importance of sitespecific chemistry is demonstrated.

KW - MONOCLONAL-ANTIBODY

KW - TARGETED DELIVERY

KW - FRAGMENTS

KW - OPTIMIZATION

KW - INSTALLATION

KW - CONJUGATION

KW - DRUGS

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DO - 10.1039/c9cc02655j

M3 - Article

SN - 1359-7345

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EP - 7674

JO - Chemical Communications

JF - Chemical Communications

IS - 53

ER -

Oriented attachment of VNAR proteins, Q2 via site-selective modification, on PLGA–PEG nanoparticles enhances nanoconjugate performance

Abstract

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Keywords

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