Osmoprotection by carnitine in a Listeria monocytogenes mutant lacking the OpuC transporter: evidence for a low affinity carnitine uptake system

Katy Fraser, C. P. O'Byrne

    Research output: Contribution to journalArticle

    11 Citations (Scopus)

    Abstract

    A deletion mutant of Listeria monocytogenes lacking OpuC, an ABC transporter responsible for the uptake of the compatible solute carnitine, was constructed and carnitine transport assays confirmed that carnitine transport was defective in this mutant. However, the mutant retained the ability to derive osmoprotection from carnitine, suggesting the presence of a second uptake system for this compatible solute. Measurement of intracellular carnitine pools during balanced growth confirmed that the opuC mutant accumulated high levels of carnitine. These pools were only achieved in the mutant when high levels (1 mM) of carnitine were present extracellularly. When a lower level (100 muM) was supplied in the medium the mutant failed to accumulate a substantial intracellular pool and failed to derive osmoprotection from carnitine. These data suggest the presence of a second low affinity carnitine uptake system in this osmotolerant pathogen. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

    Original languageEnglish
    Pages (from-to)189-194
    Number of pages5
    JournalFEMS Microbiology Letters
    Volume211
    Issue number2
    DOIs
    Publication statusPublished - 2002

    Keywords

    • OpuC
    • carnitine
    • osmoprotection
    • compatible solute
    • Listeria monocytogenes
    • SCOTT-A
    • BETAINE
    • IDENTIFICATION
    • GROWTH
    • VIRULENCE
    • TOLERANCE
    • GENES

    Cite this

    Osmoprotection by carnitine in a Listeria monocytogenes mutant lacking the OpuC transporter: evidence for a low affinity carnitine uptake system. / Fraser, Katy; O'Byrne, C. P.

    In: FEMS Microbiology Letters, Vol. 211, No. 2, 2002, p. 189-194.

    Research output: Contribution to journalArticle

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    abstract = "A deletion mutant of Listeria monocytogenes lacking OpuC, an ABC transporter responsible for the uptake of the compatible solute carnitine, was constructed and carnitine transport assays confirmed that carnitine transport was defective in this mutant. However, the mutant retained the ability to derive osmoprotection from carnitine, suggesting the presence of a second uptake system for this compatible solute. Measurement of intracellular carnitine pools during balanced growth confirmed that the opuC mutant accumulated high levels of carnitine. These pools were only achieved in the mutant when high levels (1 mM) of carnitine were present extracellularly. When a lower level (100 muM) was supplied in the medium the mutant failed to accumulate a substantial intracellular pool and failed to derive osmoprotection from carnitine. These data suggest the presence of a second low affinity carnitine uptake system in this osmotolerant pathogen. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.",
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