Overexpression of suppressor of cytokine signaling 3 in the arcuate nucleus of juvenile Phodopus sungorus alters seasonal body weight changes

Gotham K. Ganjam, Jonas Benzler, Olaf Pinkenburg, Alisa Boucsein, Sigrid Stöhr, Juliane Steger, Carsten Culmsee, Perry Barrett, Alexander Tups

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The profound seasonal cycle in body weight exhibited by the Djungarian hamster (Phodopus sungorus) is associated with the development of hypothalamic leptin resistance during long day photoperiod (LD, 16:8 h light dark cycle), when body weight is elevated relative to short day photoperiod (SD, 8:16 h light dark cycle). We previously have shown that this seasonal change in physiology is associated with higher levels of mRNA for the potent inhibitor of leptin signaling, suppressor of cytokine signaling-3 (SOCS3), in the arcuate nucleus (ARC) of LD hamsters relative to hamsters in SD. The alteration in SOCS3 gene expression preceded the body weight change suggesting that SOCS3 might be the molecular switch of seasonal body weight changes. To functionally characterize the role of SOCS3 in seasonal body weight regulation, we injected SOCS3 expressing recombinant adeno-associated virus type-2 (rAAV2-SOCS3) constructs into the ARC of leptin sensitive SD hamsters immediately after weaning. Hamsters that received rAAV2 expressing enhanced green fluorescent protein (rAAV2-EGFP) served as controls. ARC-directed SOCS3 overexpression led to a significant increase in body weight over a period of 12 weeks without fully restoring the LD phenotype. This increase was partially due to elevated brown and white adipose tissue mass. Gene expression of pro-opiomelanocortin was increased while thyroid hormone converting enzyme DIO3 mRNA levels were reduced in SD hamsters with SOCS3 overexpression. In conclusion, our data suggest that ARC-directed SOCS3 overexpression partially overcomes the profound seasonal body weight cycle exhibited by the hamster which is associated with altered pro-opiomelanocortin and DIO3 gene expression.
Original languageEnglish
Pages (from-to)1101-1111
Number of pages11
JournalJournal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology
Volume183
Issue number8
Early online date17 Jul 2013
DOIs
Publication statusPublished - Dec 2013

Fingerprint

Phodopus
Ventral Thalamic Nuclei
Body Weight Changes
body weight changes
cytokines
hamsters
Cytokines
Cricetinae
Photoperiod
Arcuate Nucleus of Hypothalamus
Body Weight
photoperiod
Leptin
leptin
body weight
pro-opiomelanocortin
Gene expression
Pro-Opiomelanocortin
gene expression
Gene Expression

Keywords

  • adeno associated-virus
  • SOCS3
  • arcuate nucleus
  • body weight
  • POMC
  • deiodinase

Cite this

Overexpression of suppressor of cytokine signaling 3 in the arcuate nucleus of juvenile Phodopus sungorus alters seasonal body weight changes. / Ganjam, Gotham K.; Benzler, Jonas; Pinkenburg, Olaf; Boucsein, Alisa; Stöhr, Sigrid; Steger, Juliane; Culmsee, Carsten; Barrett, Perry; Tups, Alexander.

In: Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology, Vol. 183, No. 8, 12.2013, p. 1101-1111.

Research output: Contribution to journalArticle

Ganjam, Gotham K. ; Benzler, Jonas ; Pinkenburg, Olaf ; Boucsein, Alisa ; Stöhr, Sigrid ; Steger, Juliane ; Culmsee, Carsten ; Barrett, Perry ; Tups, Alexander. / Overexpression of suppressor of cytokine signaling 3 in the arcuate nucleus of juvenile Phodopus sungorus alters seasonal body weight changes. In: Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology. 2013 ; Vol. 183, No. 8. pp. 1101-1111.
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AU - Barrett, Perry

AU - Tups, Alexander

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