PADI4 genotype is not associated with rheumatoid arthritis in a large UK Caucasian population

Marian L Burr, Haris Naseem, Anne Hinks, Steve Eyre, Laura J Gibbons, John Bowes, Anthony G Wilson, James Maxwell, Ann W Morgan, Paul Emery, Sophia Steer, Lynne Hocking, David M Reid, Paul Wordsworth, Pille Harrison, Wendy Thomson, Jane Worthington, Anne Barton, BIRAC consortium

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Abstract

BACKGROUND: Polymorphisms of the peptidylarginine deiminase type 4 (PADI4) gene confer susceptibility to rheumatoid arthritis (RA) in East Asian people. However, studies in European populations have produced conflicting results. This study explored the association of the PADI4 genotype with RA in a large UK Caucasian population. METHODS: The PADI4_94 (rs2240340) single nucleotide polymorphism (SNP) was directly genotyped in a cohort of unrelated UK Caucasian patients with RA (n=3732) and population controls (n=3039). Imputed data from the Wellcome Trust Case Control Consortium (WTCCC) was used to investigate the association of PADI4_94 with RA in an independent group of RA cases (n=1859) and controls (n=10 599). A further 56 SNPs spanning the PADI4 gene were investigated for association with RA using data from the WTCCC study. RESULTS: The PADI4_94 genotype was not associated with RA in either the present cohort or the WTCCC cohort. Combined analysis of all the cases of RA (n=5591) and controls (n=13 638) gave an overall OR of 1.01 (95% CI 0.96 to 1.05, p=0.72). No association with anti-CCP antibodies and no interaction with either shared epitope or PTPN22 was detected. No evidence for association with RA was identified for any of the PADI4 SNPs investigated. Meta-analysis of previously published studies and our data confirmed no significant association between the PADI4_94 genotype and RA in people of European descent (OR 1.06, 95% CI 0.99 to 1.13, p=0.12). CONCLUSION: In the largest study performed to date, the PADI4 genotype was not a significant risk factor for RA in people of European ancestry, in contrast to Asian populations.
Original languageEnglish
Pages (from-to)666-670
Number of pages5
JournalAnnals of the Rheumatic Diseases
Volume69
Issue number4
Early online date25 May 2009
DOIs
Publication statusPublished - Apr 2010

Fingerprint

Rheumatoid Arthritis
Genotype
Population
Single Nucleotide Polymorphism
Polymorphism
Genes
protein-arginine deiminase
Rheumatoid Factor
Population Control
Epitopes
Nucleotides
Meta-Analysis
Case-Control Studies
Anti-Idiotypic Antibodies
Antibodies

Keywords

  • arthritis, rheumatoid
  • autoantibodies
  • epidemiologic methods
  • European continental ancestry group
  • female
  • gene frequency
  • genetic predisposition to disease
  • genotype
  • humans
  • hydrolases
  • male
  • polymorphism, single nucleotide

Cite this

Burr, M. L., Naseem, H., Hinks, A., Eyre, S., Gibbons, L. J., Bowes, J., ... BIRAC consortium (2010). PADI4 genotype is not associated with rheumatoid arthritis in a large UK Caucasian population. Annals of the Rheumatic Diseases, 69(4), 666-670. https://doi.org/10.1136/ard.2009.111294

PADI4 genotype is not associated with rheumatoid arthritis in a large UK Caucasian population. / Burr, Marian L; Naseem, Haris; Hinks, Anne; Eyre, Steve; Gibbons, Laura J; Bowes, John; Wilson, Anthony G; Maxwell, James; Morgan, Ann W; Emery, Paul; Steer, Sophia; Hocking, Lynne; Reid, David M; Wordsworth, Paul; Harrison, Pille; Thomson, Wendy; Worthington, Jane; Barton, Anne; BIRAC consortium.

In: Annals of the Rheumatic Diseases, Vol. 69, No. 4, 04.2010, p. 666-670.

Research output: Contribution to journalArticle

Burr, ML, Naseem, H, Hinks, A, Eyre, S, Gibbons, LJ, Bowes, J, Wilson, AG, Maxwell, J, Morgan, AW, Emery, P, Steer, S, Hocking, L, Reid, DM, Wordsworth, P, Harrison, P, Thomson, W, Worthington, J, Barton, A & BIRAC consortium 2010, 'PADI4 genotype is not associated with rheumatoid arthritis in a large UK Caucasian population', Annals of the Rheumatic Diseases, vol. 69, no. 4, pp. 666-670. https://doi.org/10.1136/ard.2009.111294
Burr, Marian L ; Naseem, Haris ; Hinks, Anne ; Eyre, Steve ; Gibbons, Laura J ; Bowes, John ; Wilson, Anthony G ; Maxwell, James ; Morgan, Ann W ; Emery, Paul ; Steer, Sophia ; Hocking, Lynne ; Reid, David M ; Wordsworth, Paul ; Harrison, Pille ; Thomson, Wendy ; Worthington, Jane ; Barton, Anne ; BIRAC consortium. / PADI4 genotype is not associated with rheumatoid arthritis in a large UK Caucasian population. In: Annals of the Rheumatic Diseases. 2010 ; Vol. 69, No. 4. pp. 666-670.
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abstract = "BACKGROUND: Polymorphisms of the peptidylarginine deiminase type 4 (PADI4) gene confer susceptibility to rheumatoid arthritis (RA) in East Asian people. However, studies in European populations have produced conflicting results. This study explored the association of the PADI4 genotype with RA in a large UK Caucasian population. METHODS: The PADI4_94 (rs2240340) single nucleotide polymorphism (SNP) was directly genotyped in a cohort of unrelated UK Caucasian patients with RA (n=3732) and population controls (n=3039). Imputed data from the Wellcome Trust Case Control Consortium (WTCCC) was used to investigate the association of PADI4_94 with RA in an independent group of RA cases (n=1859) and controls (n=10 599). A further 56 SNPs spanning the PADI4 gene were investigated for association with RA using data from the WTCCC study. RESULTS: The PADI4_94 genotype was not associated with RA in either the present cohort or the WTCCC cohort. Combined analysis of all the cases of RA (n=5591) and controls (n=13 638) gave an overall OR of 1.01 (95{\%} CI 0.96 to 1.05, p=0.72). No association with anti-CCP antibodies and no interaction with either shared epitope or PTPN22 was detected. No evidence for association with RA was identified for any of the PADI4 SNPs investigated. Meta-analysis of previously published studies and our data confirmed no significant association between the PADI4_94 genotype and RA in people of European descent (OR 1.06, 95{\%} CI 0.99 to 1.13, p=0.12). CONCLUSION: In the largest study performed to date, the PADI4 genotype was not a significant risk factor for RA in people of European ancestry, in contrast to Asian populations.",
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T1 - PADI4 genotype is not associated with rheumatoid arthritis in a large UK Caucasian population

AU - Burr, Marian L

AU - Naseem, Haris

AU - Hinks, Anne

AU - Eyre, Steve

AU - Gibbons, Laura J

AU - Bowes, John

AU - Wilson, Anthony G

AU - Maxwell, James

AU - Morgan, Ann W

AU - Emery, Paul

AU - Steer, Sophia

AU - Hocking, Lynne

AU - Reid, David M

AU - Wordsworth, Paul

AU - Harrison, Pille

AU - Thomson, Wendy

AU - Worthington, Jane

AU - Barton, Anne

AU - BIRAC consortium

PY - 2010/4

Y1 - 2010/4

N2 - BACKGROUND: Polymorphisms of the peptidylarginine deiminase type 4 (PADI4) gene confer susceptibility to rheumatoid arthritis (RA) in East Asian people. However, studies in European populations have produced conflicting results. This study explored the association of the PADI4 genotype with RA in a large UK Caucasian population. METHODS: The PADI4_94 (rs2240340) single nucleotide polymorphism (SNP) was directly genotyped in a cohort of unrelated UK Caucasian patients with RA (n=3732) and population controls (n=3039). Imputed data from the Wellcome Trust Case Control Consortium (WTCCC) was used to investigate the association of PADI4_94 with RA in an independent group of RA cases (n=1859) and controls (n=10 599). A further 56 SNPs spanning the PADI4 gene were investigated for association with RA using data from the WTCCC study. RESULTS: The PADI4_94 genotype was not associated with RA in either the present cohort or the WTCCC cohort. Combined analysis of all the cases of RA (n=5591) and controls (n=13 638) gave an overall OR of 1.01 (95% CI 0.96 to 1.05, p=0.72). No association with anti-CCP antibodies and no interaction with either shared epitope or PTPN22 was detected. No evidence for association with RA was identified for any of the PADI4 SNPs investigated. Meta-analysis of previously published studies and our data confirmed no significant association between the PADI4_94 genotype and RA in people of European descent (OR 1.06, 95% CI 0.99 to 1.13, p=0.12). CONCLUSION: In the largest study performed to date, the PADI4 genotype was not a significant risk factor for RA in people of European ancestry, in contrast to Asian populations.

AB - BACKGROUND: Polymorphisms of the peptidylarginine deiminase type 4 (PADI4) gene confer susceptibility to rheumatoid arthritis (RA) in East Asian people. However, studies in European populations have produced conflicting results. This study explored the association of the PADI4 genotype with RA in a large UK Caucasian population. METHODS: The PADI4_94 (rs2240340) single nucleotide polymorphism (SNP) was directly genotyped in a cohort of unrelated UK Caucasian patients with RA (n=3732) and population controls (n=3039). Imputed data from the Wellcome Trust Case Control Consortium (WTCCC) was used to investigate the association of PADI4_94 with RA in an independent group of RA cases (n=1859) and controls (n=10 599). A further 56 SNPs spanning the PADI4 gene were investigated for association with RA using data from the WTCCC study. RESULTS: The PADI4_94 genotype was not associated with RA in either the present cohort or the WTCCC cohort. Combined analysis of all the cases of RA (n=5591) and controls (n=13 638) gave an overall OR of 1.01 (95% CI 0.96 to 1.05, p=0.72). No association with anti-CCP antibodies and no interaction with either shared epitope or PTPN22 was detected. No evidence for association with RA was identified for any of the PADI4 SNPs investigated. Meta-analysis of previously published studies and our data confirmed no significant association between the PADI4_94 genotype and RA in people of European descent (OR 1.06, 95% CI 0.99 to 1.13, p=0.12). CONCLUSION: In the largest study performed to date, the PADI4 genotype was not a significant risk factor for RA in people of European ancestry, in contrast to Asian populations.

KW - arthritis, rheumatoid

KW - autoantibodies

KW - epidemiologic methods

KW - European continental ancestry group

KW - female

KW - gene frequency

KW - genetic predisposition to disease

KW - genotype

KW - humans

KW - hydrolases

KW - male

KW - polymorphism, single nucleotide

U2 - 10.1136/ard.2009.111294

DO - 10.1136/ard.2009.111294

M3 - Article

VL - 69

SP - 666

EP - 670

JO - Annals of the Rheumatic Diseases

JF - Annals of the Rheumatic Diseases

SN - 0003-4967

IS - 4

ER -