Pancreatic stellate cells can form new β-like cells

Kevin Docherty

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Regenerative medicine, including cell-replacement strategies, may have an important role in the treatment of Type 1 and Type 2 diabetes, both of which are associated with decreased islet cell mass. To date, significant progress has been made in deriving insulin-secreting beta-like cells from human ES (embryonic stem) cells. However, the cells are not fully differentiated, and there is a long way to go before they could be used as a replenishable supply of insulin-secreting beta-cells for transplantation. For this reason, adult pancreatic stem cells are seen as an alternative source that could be expanded and differentiated ex vivo, or induced to form new islets in situ. In this issue of the Biochemical Journal, Mato et al. used drug selection to purify a population of stellate cells from explant cultures of pancreas from lactating rats. The selected cells express some stem-cell markers and can be grown for over 2 years as a fibroblast-like monolayer. When plated on extracellular matrix, along with a cocktail of growth factors that included insulin, transferrin, selenium and the GLP-1 (glucagon-like peptide-1) analogue exendin-4, the cells differentiated into cells that expressed many of the phenotypic markers characteristic of a beta-cell, and exhibited an insulin-secretory response, albeit weak, to glucose. The ability to purify this cell population opens up the possibility of unravelling the mechanisms that control self-renewal and differentiation of pancreatic cells that share some of the properties of stem cells.
Original languageEnglish
Pages (from-to)e1-e4
Number of pages4
JournalBiochemical Journal
Volume421
Issue number2
Early online date26 Jun 2009
DOIs
Publication statusPublished - 15 Jul 2009

Fingerprint

Pancreatic Stellate Cells
Stem cells
Insulin
Cells
Glucagon-Like Peptide 1
Fibroblasts
Transferrin
Medical problems
Selenium
Cell culture
Rats
Monolayers
Intercellular Signaling Peptides and Proteins
Stem Cells
Glucose
Adult Stem Cells
Regenerative Medicine
Cell Transplantation
Insulin-Secreting Cells
Islets of Langerhans

Keywords

  • animals
  • cell differentiation
  • cells, cultured
  • humans
  • insulin-secreting cells
  • mice
  • pancreas
  • rats
  • stem cells
  • cell therapy
  • diabetes mellitus
  • islet of Langerhans
  • pancreatic stem cell

Cite this

Pancreatic stellate cells can form new β-like cells. / Docherty, Kevin.

In: Biochemical Journal, Vol. 421, No. 2, 15.07.2009, p. e1-e4.

Research output: Contribution to journalArticle

Docherty, Kevin. / Pancreatic stellate cells can form new β-like cells. In: Biochemical Journal. 2009 ; Vol. 421, No. 2. pp. e1-e4.
@article{60cba8eeea364e8c869dfc21ef957b39,
title = "Pancreatic stellate cells can form new β-like cells",
abstract = "Regenerative medicine, including cell-replacement strategies, may have an important role in the treatment of Type 1 and Type 2 diabetes, both of which are associated with decreased islet cell mass. To date, significant progress has been made in deriving insulin-secreting beta-like cells from human ES (embryonic stem) cells. However, the cells are not fully differentiated, and there is a long way to go before they could be used as a replenishable supply of insulin-secreting beta-cells for transplantation. For this reason, adult pancreatic stem cells are seen as an alternative source that could be expanded and differentiated ex vivo, or induced to form new islets in situ. In this issue of the Biochemical Journal, Mato et al. used drug selection to purify a population of stellate cells from explant cultures of pancreas from lactating rats. The selected cells express some stem-cell markers and can be grown for over 2 years as a fibroblast-like monolayer. When plated on extracellular matrix, along with a cocktail of growth factors that included insulin, transferrin, selenium and the GLP-1 (glucagon-like peptide-1) analogue exendin-4, the cells differentiated into cells that expressed many of the phenotypic markers characteristic of a beta-cell, and exhibited an insulin-secretory response, albeit weak, to glucose. The ability to purify this cell population opens up the possibility of unravelling the mechanisms that control self-renewal and differentiation of pancreatic cells that share some of the properties of stem cells.",
keywords = "animals, cell differentiation, cells, cultured, humans, insulin-secreting cells, mice, pancreas, rats, stem cells , cell therapy, diabetes mellitus, islet of Langerhans, pancreatic stem cell",
author = "Kevin Docherty",
year = "2009",
month = "7",
day = "15",
doi = "10.1042/BJ20090779",
language = "English",
volume = "421",
pages = "e1--e4",
journal = "Biochemical Journal",
issn = "0264-6021",
publisher = "Portland Press Ltd.",
number = "2",

}

TY - JOUR

T1 - Pancreatic stellate cells can form new β-like cells

AU - Docherty, Kevin

PY - 2009/7/15

Y1 - 2009/7/15

N2 - Regenerative medicine, including cell-replacement strategies, may have an important role in the treatment of Type 1 and Type 2 diabetes, both of which are associated with decreased islet cell mass. To date, significant progress has been made in deriving insulin-secreting beta-like cells from human ES (embryonic stem) cells. However, the cells are not fully differentiated, and there is a long way to go before they could be used as a replenishable supply of insulin-secreting beta-cells for transplantation. For this reason, adult pancreatic stem cells are seen as an alternative source that could be expanded and differentiated ex vivo, or induced to form new islets in situ. In this issue of the Biochemical Journal, Mato et al. used drug selection to purify a population of stellate cells from explant cultures of pancreas from lactating rats. The selected cells express some stem-cell markers and can be grown for over 2 years as a fibroblast-like monolayer. When plated on extracellular matrix, along with a cocktail of growth factors that included insulin, transferrin, selenium and the GLP-1 (glucagon-like peptide-1) analogue exendin-4, the cells differentiated into cells that expressed many of the phenotypic markers characteristic of a beta-cell, and exhibited an insulin-secretory response, albeit weak, to glucose. The ability to purify this cell population opens up the possibility of unravelling the mechanisms that control self-renewal and differentiation of pancreatic cells that share some of the properties of stem cells.

AB - Regenerative medicine, including cell-replacement strategies, may have an important role in the treatment of Type 1 and Type 2 diabetes, both of which are associated with decreased islet cell mass. To date, significant progress has been made in deriving insulin-secreting beta-like cells from human ES (embryonic stem) cells. However, the cells are not fully differentiated, and there is a long way to go before they could be used as a replenishable supply of insulin-secreting beta-cells for transplantation. For this reason, adult pancreatic stem cells are seen as an alternative source that could be expanded and differentiated ex vivo, or induced to form new islets in situ. In this issue of the Biochemical Journal, Mato et al. used drug selection to purify a population of stellate cells from explant cultures of pancreas from lactating rats. The selected cells express some stem-cell markers and can be grown for over 2 years as a fibroblast-like monolayer. When plated on extracellular matrix, along with a cocktail of growth factors that included insulin, transferrin, selenium and the GLP-1 (glucagon-like peptide-1) analogue exendin-4, the cells differentiated into cells that expressed many of the phenotypic markers characteristic of a beta-cell, and exhibited an insulin-secretory response, albeit weak, to glucose. The ability to purify this cell population opens up the possibility of unravelling the mechanisms that control self-renewal and differentiation of pancreatic cells that share some of the properties of stem cells.

KW - animals

KW - cell differentiation

KW - cells, cultured

KW - humans

KW - insulin-secreting cells

KW - mice

KW - pancreas

KW - rats

KW - stem cells

KW - cell therapy

KW - diabetes mellitus

KW - islet of Langerhans

KW - pancreatic stem cell

U2 - 10.1042/BJ20090779

DO - 10.1042/BJ20090779

M3 - Article

VL - 421

SP - e1-e4

JO - Biochemical Journal

JF - Biochemical Journal

SN - 0264-6021

IS - 2

ER -