Parallel observation of four methods for screening women under 25 years of age for genital infection with Chlamydia trachomatis

Hamish McKenzie, Alexander Allan Templeton

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Objectives: To compare four methods of screening women for Chlamydia trachomatis in an obstetrics and gynaccology department. Study design: A total of 303 healthy women under 25 years were recruited from antenatal, induced abortion, and family planning clinics. Each underwent parallel testing of endocervical specimens by enzyme immunoassay (EIA) and ligase chain reaction (LCR), vulval swabs by LCR, and urine by LCR. Outcome measures included sensitivity, specificity, acceptability of each method, and the influence of pregnancy. Results: Overall prevalence (95% CI) was 9.9% (6.8-14%). All methods had a high rate of detection (75-100%), not affected by pregnancy. Urine was most acceptable, followed by vulval swabbing. Conclusions: Opportunistic screening of women under 25 years attending obstetric and gynaecology affiliated clinics found high rates of C. trachomatis infection. Both urine and vulval swab methods were highly sensitive, acceptable, and not affected by pregnancy status. Due to pragmatic issues surrounding the urine method, screening by vulval swabs deserves wider recognition. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)68-73
Number of pages5
JournalEuropean Journal of Obstetrics & Gynecology and Reproductive Biology
Volume107
Issue number1
DOIs
Publication statusPublished - 2003

Keywords

  • acceptability
  • Chlamydia trachomatis
  • EIA
  • LCR
  • vulval swabs
  • LIGASE CHAIN-REACTION
  • REACTION ASSAY
  • DIAGNOSIS
  • URINE
  • SPECIMENS
  • SAMPLES
  • TESTS
  • SWABS

Cite this

@article{f0ca0555391b4f958d0842260054da71,
title = "Parallel observation of four methods for screening women under 25 years of age for genital infection with Chlamydia trachomatis",
abstract = "Objectives: To compare four methods of screening women for Chlamydia trachomatis in an obstetrics and gynaccology department. Study design: A total of 303 healthy women under 25 years were recruited from antenatal, induced abortion, and family planning clinics. Each underwent parallel testing of endocervical specimens by enzyme immunoassay (EIA) and ligase chain reaction (LCR), vulval swabs by LCR, and urine by LCR. Outcome measures included sensitivity, specificity, acceptability of each method, and the influence of pregnancy. Results: Overall prevalence (95{\%} CI) was 9.9{\%} (6.8-14{\%}). All methods had a high rate of detection (75-100{\%}), not affected by pregnancy. Urine was most acceptable, followed by vulval swabbing. Conclusions: Opportunistic screening of women under 25 years attending obstetric and gynaecology affiliated clinics found high rates of C. trachomatis infection. Both urine and vulval swab methods were highly sensitive, acceptable, and not affected by pregnancy status. Due to pragmatic issues surrounding the urine method, screening by vulval swabs deserves wider recognition. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.",
keywords = "acceptability, Chlamydia trachomatis, EIA, LCR, vulval swabs, LIGASE CHAIN-REACTION, REACTION ASSAY, DIAGNOSIS, URINE, SPECIMENS, SAMPLES, TESTS, SWABS",
author = "Hamish McKenzie and Templeton, {Alexander Allan}",
year = "2003",
doi = "10.1016/S0301-2115(02)00266-X",
language = "English",
volume = "107",
pages = "68--73",
journal = "European Journal of Obstetrics & Gynecology and Reproductive Biology",
issn = "0301-2115",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

TY - JOUR

T1 - Parallel observation of four methods for screening women under 25 years of age for genital infection with Chlamydia trachomatis

AU - McKenzie, Hamish

AU - Templeton, Alexander Allan

PY - 2003

Y1 - 2003

N2 - Objectives: To compare four methods of screening women for Chlamydia trachomatis in an obstetrics and gynaccology department. Study design: A total of 303 healthy women under 25 years were recruited from antenatal, induced abortion, and family planning clinics. Each underwent parallel testing of endocervical specimens by enzyme immunoassay (EIA) and ligase chain reaction (LCR), vulval swabs by LCR, and urine by LCR. Outcome measures included sensitivity, specificity, acceptability of each method, and the influence of pregnancy. Results: Overall prevalence (95% CI) was 9.9% (6.8-14%). All methods had a high rate of detection (75-100%), not affected by pregnancy. Urine was most acceptable, followed by vulval swabbing. Conclusions: Opportunistic screening of women under 25 years attending obstetric and gynaecology affiliated clinics found high rates of C. trachomatis infection. Both urine and vulval swab methods were highly sensitive, acceptable, and not affected by pregnancy status. Due to pragmatic issues surrounding the urine method, screening by vulval swabs deserves wider recognition. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

AB - Objectives: To compare four methods of screening women for Chlamydia trachomatis in an obstetrics and gynaccology department. Study design: A total of 303 healthy women under 25 years were recruited from antenatal, induced abortion, and family planning clinics. Each underwent parallel testing of endocervical specimens by enzyme immunoassay (EIA) and ligase chain reaction (LCR), vulval swabs by LCR, and urine by LCR. Outcome measures included sensitivity, specificity, acceptability of each method, and the influence of pregnancy. Results: Overall prevalence (95% CI) was 9.9% (6.8-14%). All methods had a high rate of detection (75-100%), not affected by pregnancy. Urine was most acceptable, followed by vulval swabbing. Conclusions: Opportunistic screening of women under 25 years attending obstetric and gynaecology affiliated clinics found high rates of C. trachomatis infection. Both urine and vulval swab methods were highly sensitive, acceptable, and not affected by pregnancy status. Due to pragmatic issues surrounding the urine method, screening by vulval swabs deserves wider recognition. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

KW - acceptability

KW - Chlamydia trachomatis

KW - EIA

KW - LCR

KW - vulval swabs

KW - LIGASE CHAIN-REACTION

KW - REACTION ASSAY

KW - DIAGNOSIS

KW - URINE

KW - SPECIMENS

KW - SAMPLES

KW - TESTS

KW - SWABS

U2 - 10.1016/S0301-2115(02)00266-X

DO - 10.1016/S0301-2115(02)00266-X

M3 - Article

VL - 107

SP - 68

EP - 73

JO - European Journal of Obstetrics & Gynecology and Reproductive Biology

JF - European Journal of Obstetrics & Gynecology and Reproductive Biology

SN - 0301-2115

IS - 1

ER -