Parallel observation of four methods for screening women under 25 years of age for genital infection with Chlamydia trachomatis

Hamish McKenzie; Alexander Allan Templeton

doi:10.1016/S0301-2115(02)00266-X

Parallel observation of four methods for screening women under 25 years of age for genital infection with Chlamydia trachomatis

Hamish McKenzie, Alexander Allan Templeton

Applied Health Sciences

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

Objectives: To compare four methods of screening women for Chlamydia trachomatis in an obstetrics and gynaccology department. Study design: A total of 303 healthy women under 25 years were recruited from antenatal, induced abortion, and family planning clinics. Each underwent parallel testing of endocervical specimens by enzyme immunoassay (EIA) and ligase chain reaction (LCR), vulval swabs by LCR, and urine by LCR. Outcome measures included sensitivity, specificity, acceptability of each method, and the influence of pregnancy. Results: Overall prevalence (95% CI) was 9.9% (6.8-14%). All methods had a high rate of detection (75-100%), not affected by pregnancy. Urine was most acceptable, followed by vulval swabbing. Conclusions: Opportunistic screening of women under 25 years attending obstetric and gynaecology affiliated clinics found high rates of C. trachomatis infection. Both urine and vulval swab methods were highly sensitive, acceptable, and not affected by pregnancy status. Due to pragmatic issues surrounding the urine method, screening by vulval swabs deserves wider recognition. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

Original language	English
Pages (from-to)	68-73
Number of pages	5
Journal	European Journal of Obstetrics & Gynecology and Reproductive Biology
Volume	107
Issue number	1
DOIs	https://doi.org/10.1016/S0301-2115(02)00266-X
Publication status	Published - 2003

Bibliographical note

Acknowledgements
We wish to thank all the women who volunteered to be tested. We acknowledge the goodwill and cooperation of the nursing, midwifery, and medical staff from the Department of Obstetrics and Gynaecology and Family Planning Service in Aberdeen. We also thank Mrs. J. Skinner and Mrs. L. Ford for excellent technical assistance and Abbott Diagnostics, UK for performing the LCR-MOMP assays. We are grateful for the statistical assistance provided by Ann Fitzmaurice.

Keywords

acceptability
Chlamydia trachomatis
EIA
LCR
vulval swabs
LIGASE CHAIN-REACTION
REACTION ASSAY
DIAGNOSIS
URINE
SPECIMENS
SAMPLES
TESTS
SWABS

Access to Document

10.1016/S0301-2115(02)00266-X

Cite this

@article{f0ca0555391b4f958d0842260054da71,

title = "Parallel observation of four methods for screening women under 25 years of age for genital infection with Chlamydia trachomatis",

abstract = "Objectives: To compare four methods of screening women for Chlamydia trachomatis in an obstetrics and gynaccology department. Study design: A total of 303 healthy women under 25 years were recruited from antenatal, induced abortion, and family planning clinics. Each underwent parallel testing of endocervical specimens by enzyme immunoassay (EIA) and ligase chain reaction (LCR), vulval swabs by LCR, and urine by LCR. Outcome measures included sensitivity, specificity, acceptability of each method, and the influence of pregnancy. Results: Overall prevalence (95% CI) was 9.9% (6.8-14%). All methods had a high rate of detection (75-100%), not affected by pregnancy. Urine was most acceptable, followed by vulval swabbing. Conclusions: Opportunistic screening of women under 25 years attending obstetric and gynaecology affiliated clinics found high rates of C. trachomatis infection. Both urine and vulval swab methods were highly sensitive, acceptable, and not affected by pregnancy status. Due to pragmatic issues surrounding the urine method, screening by vulval swabs deserves wider recognition. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.",

keywords = "acceptability, Chlamydia trachomatis, EIA, LCR, vulval swabs, LIGASE CHAIN-REACTION, REACTION ASSAY, DIAGNOSIS, URINE, SPECIMENS, SAMPLES, TESTS, SWABS",

author = "Hamish McKenzie and Templeton, {Alexander Allan}",

note = "Acknowledgements We wish to thank all the women who volunteered to be tested. We acknowledge the goodwill and cooperation of the nursing, midwifery, and medical staff from the Department of Obstetrics and Gynaecology and Family Planning Service in Aberdeen. We also thank Mrs. J. Skinner and Mrs. L. Ford for excellent technical assistance and Abbott Diagnostics, UK for performing the LCR-MOMP assays. We are grateful for the statistical assistance provided by Ann Fitzmaurice.",

year = "2003",

doi = "10.1016/S0301-2115(02)00266-X",

language = "English",

volume = "107",

pages = "68--73",

journal = "European Journal of Obstetrics & Gynecology and Reproductive Biology",

issn = "0301-2115",

publisher = "Elsevier Ireland Ltd",

number = "1",

}

TY - JOUR

T1 - Parallel observation of four methods for screening women under 25 years of age for genital infection with Chlamydia trachomatis

AU - McKenzie, Hamish

AU - Templeton, Alexander Allan

N1 - Acknowledgements We wish to thank all the women who volunteered to be tested. We acknowledge the goodwill and cooperation of the nursing, midwifery, and medical staff from the Department of Obstetrics and Gynaecology and Family Planning Service in Aberdeen. We also thank Mrs. J. Skinner and Mrs. L. Ford for excellent technical assistance and Abbott Diagnostics, UK for performing the LCR-MOMP assays. We are grateful for the statistical assistance provided by Ann Fitzmaurice.

PY - 2003

Y1 - 2003

N2 - Objectives: To compare four methods of screening women for Chlamydia trachomatis in an obstetrics and gynaccology department. Study design: A total of 303 healthy women under 25 years were recruited from antenatal, induced abortion, and family planning clinics. Each underwent parallel testing of endocervical specimens by enzyme immunoassay (EIA) and ligase chain reaction (LCR), vulval swabs by LCR, and urine by LCR. Outcome measures included sensitivity, specificity, acceptability of each method, and the influence of pregnancy. Results: Overall prevalence (95% CI) was 9.9% (6.8-14%). All methods had a high rate of detection (75-100%), not affected by pregnancy. Urine was most acceptable, followed by vulval swabbing. Conclusions: Opportunistic screening of women under 25 years attending obstetric and gynaecology affiliated clinics found high rates of C. trachomatis infection. Both urine and vulval swab methods were highly sensitive, acceptable, and not affected by pregnancy status. Due to pragmatic issues surrounding the urine method, screening by vulval swabs deserves wider recognition. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

AB - Objectives: To compare four methods of screening women for Chlamydia trachomatis in an obstetrics and gynaccology department. Study design: A total of 303 healthy women under 25 years were recruited from antenatal, induced abortion, and family planning clinics. Each underwent parallel testing of endocervical specimens by enzyme immunoassay (EIA) and ligase chain reaction (LCR), vulval swabs by LCR, and urine by LCR. Outcome measures included sensitivity, specificity, acceptability of each method, and the influence of pregnancy. Results: Overall prevalence (95% CI) was 9.9% (6.8-14%). All methods had a high rate of detection (75-100%), not affected by pregnancy. Urine was most acceptable, followed by vulval swabbing. Conclusions: Opportunistic screening of women under 25 years attending obstetric and gynaecology affiliated clinics found high rates of C. trachomatis infection. Both urine and vulval swab methods were highly sensitive, acceptable, and not affected by pregnancy status. Due to pragmatic issues surrounding the urine method, screening by vulval swabs deserves wider recognition. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

KW - acceptability

KW - Chlamydia trachomatis

KW - EIA

KW - LCR

KW - vulval swabs

KW - LIGASE CHAIN-REACTION

KW - REACTION ASSAY

KW - DIAGNOSIS

KW - URINE

KW - SPECIMENS

KW - SAMPLES

KW - TESTS

KW - SWABS

U2 - 10.1016/S0301-2115(02)00266-X

DO - 10.1016/S0301-2115(02)00266-X

M3 - Article

SN - 0301-2115

VL - 107

SP - 68

EP - 73

JO - European Journal of Obstetrics & Gynecology and Reproductive Biology

JF - European Journal of Obstetrics & Gynecology and Reproductive Biology

IS - 1

ER -

Parallel observation of four methods for screening women under 25 years of age for genital infection with Chlamydia trachomatis

Abstract

Bibliographical note

Keywords

Access to Document

Fingerprint

Cite this