Paraoxonase-1 Q192R polymorphism and its association with hs-CRP and fasting blood glucose levels and risk of coronary artery disease

M. Amini; S. Esmaeilzadeh-bahabadi; A. Avan; A. Gholoobi; F. Ghasemi; S.R. Mirhafez; H. Ghazizadeh; M. Moohebati; M. Ebrahimi; G.A. Ferns; A. Pasdar; M.G. Mobarhan

doi:10.1016/j.dsx.2019.01.010

Paraoxonase-1 Q192R polymorphism and its association with hs-CRP and fasting blood glucose levels and risk of coronary artery disease

M. Amini, S. Esmaeilzadeh-bahabadi, A. Avan, A. Gholoobi, F. Ghasemi, S.R. Mirhafez, H. Ghazizadeh, M. Moohebati, M. Ebrahimi, G.A. Ferns, A. Pasdar^* (Corresponding Author), M.G. Mobarhan^* (Corresponding Author)

^*Corresponding author for this work

Applied Medicine

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Aims
Paraoxonase-1 (PON1) has been shown to protect low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) against oxidative-modification and thereby might protect against coronary-artery-disease (CAD). Here we explored the relationship of a genetic variant (a substitution (R) Arg with (Q) Gln at position 192) of PON1 in 250 patients with/without CAD.

Materials and methods
Genotyping of PON1 Q192R was carried out using Real-Time-PCR TaqMan-based-probe. Demographic-characteristics and biochemical-analyses, including fasting blood sugar (FBS), HDL, LDL, triglycerides (TG) and C-reactive protein (CRP) were evaluated. Univariate/multivariate analyses were performed to determine the association of the genetic polymorphism and CAD as well as with clinical-characteristics of population.

Results
Our findings showed that RR-genotype was more frequent in CAD-patients, compared to the wild-type genotype. Moreover, CAD patients with RR-genotype had an odd ratio of 5.0 (95% CI: 1.3–18.6; p = 0.017), versus wild-type genotype, in multivariate-analysis. Of note we also observed that CAD-patients with QQ-genotype had a significantly lower Hs-CRP level, compared to the RR-genotype.

Conclusion
we demonstrate that PON1-Q192R-polymorphism was associated with CRP and FBS levels; R-allele of PON1-Q192R may be an independent risk factor for CAD. Further studies are warranted to determine the value of this marker as a surrogate marker in CAD patients.

Original language	English
Pages (from-to)	1053-1057
Journal	Diabetes and Metabolic Syndrome: Clinical Research and Reviews
Volume	13
Issue number	2
Early online date	19 Jan 2019
DOIs	https://doi.org/10.1016/j.dsx.2019.01.010
Publication status	Published - Mar 2019

Keywords

Coronary artery disease
Polymorphism
Paraoxonase
Real time PCR

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Amini, M., Esmaeilzadeh-bahabadi, S., Avan, A., Gholoobi, A., Ghasemi, F., Mirhafez, S. R., Ghazizadeh, H., Moohebati, M., Ebrahimi, M., Ferns, G. A., Pasdar, A., & Mobarhan, M. G. (2019). Paraoxonase-1 Q192R polymorphism and its association with hs-CRP and fasting blood glucose levels and risk of coronary artery disease. Diabetes and Metabolic Syndrome: Clinical Research and Reviews, 13(2), 1053-1057. https://doi.org/10.1016/j.dsx.2019.01.010

Amini, M, Esmaeilzadeh-bahabadi, S, Avan, A, Gholoobi, A, Ghasemi, F, Mirhafez, SR, Ghazizadeh, H, Moohebati, M, Ebrahimi, M, Ferns, GA, Pasdar, A & Mobarhan, MG 2019, 'Paraoxonase-1 Q192R polymorphism and its association with hs-CRP and fasting blood glucose levels and risk of coronary artery disease', Diabetes and Metabolic Syndrome: Clinical Research and Reviews, vol. 13, no. 2, pp. 1053-1057. https://doi.org/10.1016/j.dsx.2019.01.010

@article{3d93c38190d74f888c2af4680d69821f,

title = "Paraoxonase-1 Q192R polymorphism and its association with hs-CRP and fasting blood glucose levels and risk of coronary artery disease",

abstract = "AimsParaoxonase-1 (PON1) has been shown to protect low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) against oxidative-modification and thereby might protect against coronary-artery-disease (CAD). Here we explored the relationship of a genetic variant (a substitution (R) Arg with (Q) Gln at position 192) of PON1 in 250 patients with/without CAD.Materials and methodsGenotyping of PON1 Q192R was carried out using Real-Time-PCR TaqMan-based-probe. Demographic-characteristics and biochemical-analyses, including fasting blood sugar (FBS), HDL, LDL, triglycerides (TG) and C-reactive protein (CRP) were evaluated. Univariate/multivariate analyses were performed to determine the association of the genetic polymorphism and CAD as well as with clinical-characteristics of population.ResultsOur findings showed that RR-genotype was more frequent in CAD-patients, compared to the wild-type genotype. Moreover, CAD patients with RR-genotype had an odd ratio of 5.0 (95% CI: 1.3–18.6; p = 0.017), versus wild-type genotype, in multivariate-analysis. Of note we also observed that CAD-patients with QQ-genotype had a significantly lower Hs-CRP level, compared to the RR-genotype.Conclusionwe demonstrate that PON1-Q192R-polymorphism was associated with CRP and FBS levels; R-allele of PON1-Q192R may be an independent risk factor for CAD. Further studies are warranted to determine the value of this marker as a surrogate marker in CAD patients.",

keywords = "Coronary artery disease, Polymorphism, Paraoxonase, Real time PCR",

author = "M. Amini and S. Esmaeilzadeh-bahabadi and A. Avan and A. Gholoobi and F. Ghasemi and S.R. Mirhafez and H. Ghazizadeh and M. Moohebati and M. Ebrahimi and G.A. Ferns and A. Pasdar and M.G. Mobarhan",

note = "Grant: This study was supported by a grant from Mashhad University of Medical Sciences (Grant number: 930834). Acknowledgments: We would like to thank Mashhad University of Medical Science Research Council for their financial supports",

year = "2019",

month = mar,

doi = "10.1016/j.dsx.2019.01.010",

language = "English",

volume = "13",

pages = "1053--1057",

journal = "Diabetes and Metabolic Syndrome: Clinical Research and Reviews",

issn = "1871-4021",

publisher = "Elsevier BV",

number = "2",

}

TY - JOUR

T1 - Paraoxonase-1 Q192R polymorphism and its association with hs-CRP and fasting blood glucose levels and risk of coronary artery disease

AU - Amini, M.

AU - Esmaeilzadeh-bahabadi, S.

AU - Avan, A.

AU - Gholoobi, A.

AU - Ghasemi, F.

AU - Mirhafez, S.R.

AU - Ghazizadeh, H.

AU - Moohebati, M.

AU - Ebrahimi, M.

AU - Ferns, G.A.

AU - Pasdar, A.

AU - Mobarhan, M.G.

N1 - Grant: This study was supported by a grant from Mashhad University of Medical Sciences (Grant number: 930834). Acknowledgments: We would like to thank Mashhad University of Medical Science Research Council for their financial supports

PY - 2019/3

Y1 - 2019/3

N2 - AimsParaoxonase-1 (PON1) has been shown to protect low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) against oxidative-modification and thereby might protect against coronary-artery-disease (CAD). Here we explored the relationship of a genetic variant (a substitution (R) Arg with (Q) Gln at position 192) of PON1 in 250 patients with/without CAD.Materials and methodsGenotyping of PON1 Q192R was carried out using Real-Time-PCR TaqMan-based-probe. Demographic-characteristics and biochemical-analyses, including fasting blood sugar (FBS), HDL, LDL, triglycerides (TG) and C-reactive protein (CRP) were evaluated. Univariate/multivariate analyses were performed to determine the association of the genetic polymorphism and CAD as well as with clinical-characteristics of population.ResultsOur findings showed that RR-genotype was more frequent in CAD-patients, compared to the wild-type genotype. Moreover, CAD patients with RR-genotype had an odd ratio of 5.0 (95% CI: 1.3–18.6; p = 0.017), versus wild-type genotype, in multivariate-analysis. Of note we also observed that CAD-patients with QQ-genotype had a significantly lower Hs-CRP level, compared to the RR-genotype.Conclusionwe demonstrate that PON1-Q192R-polymorphism was associated with CRP and FBS levels; R-allele of PON1-Q192R may be an independent risk factor for CAD. Further studies are warranted to determine the value of this marker as a surrogate marker in CAD patients.

AB - AimsParaoxonase-1 (PON1) has been shown to protect low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) against oxidative-modification and thereby might protect against coronary-artery-disease (CAD). Here we explored the relationship of a genetic variant (a substitution (R) Arg with (Q) Gln at position 192) of PON1 in 250 patients with/without CAD.Materials and methodsGenotyping of PON1 Q192R was carried out using Real-Time-PCR TaqMan-based-probe. Demographic-characteristics and biochemical-analyses, including fasting blood sugar (FBS), HDL, LDL, triglycerides (TG) and C-reactive protein (CRP) were evaluated. Univariate/multivariate analyses were performed to determine the association of the genetic polymorphism and CAD as well as with clinical-characteristics of population.ResultsOur findings showed that RR-genotype was more frequent in CAD-patients, compared to the wild-type genotype. Moreover, CAD patients with RR-genotype had an odd ratio of 5.0 (95% CI: 1.3–18.6; p = 0.017), versus wild-type genotype, in multivariate-analysis. Of note we also observed that CAD-patients with QQ-genotype had a significantly lower Hs-CRP level, compared to the RR-genotype.Conclusionwe demonstrate that PON1-Q192R-polymorphism was associated with CRP and FBS levels; R-allele of PON1-Q192R may be an independent risk factor for CAD. Further studies are warranted to determine the value of this marker as a surrogate marker in CAD patients.

KW - Coronary artery disease

KW - Polymorphism

KW - Paraoxonase

KW - Real time PCR

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U2 - 10.1016/j.dsx.2019.01.010

DO - 10.1016/j.dsx.2019.01.010

M3 - Article

VL - 13

SP - 1053

EP - 1057

JO - Diabetes and Metabolic Syndrome: Clinical Research and Reviews

JF - Diabetes and Metabolic Syndrome: Clinical Research and Reviews

SN - 1871-4021

IS - 2

ER -

Paraoxonase-1 Q192R polymorphism and its association with hs-CRP and fasting blood glucose levels and risk of coronary artery disease

Abstract

Keywords

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