Patch-clamp experiments with porins extracted from a marine bacterium (Photobacterium profundum strain SS9) and reconstituted in liposomes

Alistar Gordon MacDonald, B. Martinac, D. H. Bartlett

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    The reconstitution of bacterial porins in liposome bilayers for patch-clamp recording is well established. However, the solutions used in the dehydration, rehydration, and osmotic swelling of the liposomes have been developed for porins from enteric bacteria. Porins from marine bacteria normally function in contact with seawater whose ionic composition and osmotic pressure would appear to be incompatible with the established methods. Here, we show that, contrary to expectation, an established reconstitution and patch-clamp method works well with porins, mainly OmpH and OmpL, extracted from the deep-sea marine bacterium Photobacterium profundum strain SS9 and that seawater can be introduced at a supplementary stage.

    Original languageEnglish
    Pages (from-to)157-167
    Number of pages10
    JournalCell Biochemistry and Biophysics
    Volume37
    Issue number3
    DOIs
    Publication statusPublished - 2003

    Keywords

    • porin
    • patch clamp
    • deep-sea bacteria
    • high pressure
    • cadaverine
    • ESCHERICHIA-COLI PORIN
    • OMPC PORIN
    • MEMBRANE CHANNELS
    • ION CHANNELS
    • VOLTAGE
    • PRESSURE
    • PROTEIN
    • MUTATIONS
    • BARREL
    • GENE

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