Pathogenic Fungi Regulate Immunity by Inducing Neutrophilic Myeloid-Derived Suppressor Cells

Nikolaus Rieber, Anurag Singh, Hasan Oz, Melanie Carevic, Maria Bouzani, Jorge Amich, Michael Ost, Zhiyong Ye, Marlene Ballbach, Iris Schäfer, Markus Mezger, Sascha Klimosch, Alexander N.R. Weber, Rupert Handgretinger, Sven Krappman, Johannes Liese, Maik Engeholm, Rebecca Schüle, Helmut Rainer Salih, Laszlo Marodi & 7 others Carsten Speckmann, Bodo Grimbacher, Jürgen Ruland, Gordon D Brown, Andreas Beilhack, Juergen Loeffler, Dominik Hartl

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Abstract

Despite continuous contact with fungi, immunocompetent individuals rarely develop pro-inflammatory antifungal immune responses. The underlying tolerogenic mechanisms are incompletely understood. Using both mouse models and human patients, we show that infection with the human pathogenic fungi Aspergillus fumigatus and Candida albicans induces a distinct subset of neutrophilic myeloid-derived suppressor cells (MDSCs), which functionally suppress T and NK cell responses. Mechanistically, pathogenic fungi induce neutrophilic MDSCs through the pattern recognition receptor Dectin-1 and its downstream adaptor protein CARD9. Fungal MDSC induction is further dependent on pathways downstream of Dectin-1 signaling, notably reactive oxygen species (ROS) generation as well as caspase-8 activity and interleukin-1 (IL-1) production. Additionally, exogenous IL-1β induces MDSCs to comparable levels observed during C. albicans infection. Adoptive transfer and survival experiments show that MDSCs are protective during invasive C. albicans infection, but not A. fumigatus infection. These studies define an innate immune mechanism by which pathogenic fungi regulate host defense.
Original languageEnglish
Pages (from-to)507-514
Number of pages8
JournalCell Host & Microbe
Volume17
Issue number4
Early online date12 Mar 2015
DOIs
Publication statusPublished - Apr 2015

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Immunity
Fungi
Candida albicans
Aspergillus fumigatus
Infection
Interleukin-1
Pattern Recognition Receptors
Adoptive Transfer
Caspase 8
Natural Killer Cells
Reactive Oxygen Species
Myeloid-Derived Suppressor Cells
T-Lymphocytes
Survival
Proteins
dectin 1

Cite this

Rieber, N., Singh, A., Oz, H., Carevic, M., Bouzani, M., Amich, J., ... Hartl, D. (2015). Pathogenic Fungi Regulate Immunity by Inducing Neutrophilic Myeloid-Derived Suppressor Cells. Cell Host & Microbe, 17(4), 507-514. https://doi.org/10.1016/j.chom.2015.02.007

Pathogenic Fungi Regulate Immunity by Inducing Neutrophilic Myeloid-Derived Suppressor Cells. / Rieber, Nikolaus; Singh, Anurag; Oz, Hasan ; Carevic, Melanie; Bouzani, Maria; Amich, Jorge; Ost, Michael ; Ye, Zhiyong; Ballbach, Marlene; Schäfer, Iris; Mezger, Markus; Klimosch, Sascha; Weber, Alexander N.R.; Handgretinger, Rupert; Krappman, Sven; Liese, Johannes; Engeholm, Maik; Schüle, Rebecca; Salih, Helmut Rainer; Marodi, Laszlo; Speckmann, Carsten; Grimbacher, Bodo; Ruland, Jürgen; Brown, Gordon D; Beilhack, Andreas; Loeffler, Juergen; Hartl, Dominik.

In: Cell Host & Microbe, Vol. 17, No. 4, 04.2015, p. 507-514.

Research output: Contribution to journalArticle

Rieber, N, Singh, A, Oz, H, Carevic, M, Bouzani, M, Amich, J, Ost, M, Ye, Z, Ballbach, M, Schäfer, I, Mezger, M, Klimosch, S, Weber, ANR, Handgretinger, R, Krappman, S, Liese, J, Engeholm, M, Schüle, R, Salih, HR, Marodi, L, Speckmann, C, Grimbacher, B, Ruland, J, Brown, GD, Beilhack, A, Loeffler, J & Hartl, D 2015, 'Pathogenic Fungi Regulate Immunity by Inducing Neutrophilic Myeloid-Derived Suppressor Cells', Cell Host & Microbe, vol. 17, no. 4, pp. 507-514. https://doi.org/10.1016/j.chom.2015.02.007
Rieber, Nikolaus ; Singh, Anurag ; Oz, Hasan ; Carevic, Melanie ; Bouzani, Maria ; Amich, Jorge ; Ost, Michael ; Ye, Zhiyong ; Ballbach, Marlene ; Schäfer, Iris ; Mezger, Markus ; Klimosch, Sascha ; Weber, Alexander N.R. ; Handgretinger, Rupert ; Krappman, Sven ; Liese, Johannes ; Engeholm, Maik ; Schüle, Rebecca ; Salih, Helmut Rainer ; Marodi, Laszlo ; Speckmann, Carsten ; Grimbacher, Bodo ; Ruland, Jürgen ; Brown, Gordon D ; Beilhack, Andreas ; Loeffler, Juergen ; Hartl, Dominik. / Pathogenic Fungi Regulate Immunity by Inducing Neutrophilic Myeloid-Derived Suppressor Cells. In: Cell Host & Microbe. 2015 ; Vol. 17, No. 4. pp. 507-514.
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