Peculiar gene organisation and incomplete splicing of sea bass (Dicentrarchus labrax L.) interleukin-1b.

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23 Citations (Scopus)

Abstract

In this paper we describe the gene organisation of sea bass Dicentrarchus labrax interleukin-lbeta (IL-1beta) and the presence of incompletely spliced forms of this gene. Interestingly, the sea bass IL-1beta gene comprises five exons and four introns, thus being remarkably different from other known teleost and mammalian genes. The sizes of the introns were typically much shorter than in mammals and this feature, together with the loss of two introns, gave a Much smaller gene than in mammals (2.7 vs 6.5-7.0 kb). Tile 5'-end exon-intron organisation seems quite different in the known IL-1beta genes with three exons in human and carp, two in trout and only one in sea bass. The highest percentage of exon identity and similarity is between sea bass exon 4, trout exon 5, carp, mouse, chicken, pig and human exon 6. RT-PCR analysis revealed the presence of two incompletely spliced transcripts. Southern blot analysis suggests the presence of only one copy of IL-1beta gene in sea bass genome. (C) 2003 Elsevier Science Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)257-264
Number of pages7
JournalCytokine
Volume21
DOIs
Publication statusPublished - 2003

Keywords

  • gene organisation
  • incomplete splicing
  • interleukin-1 beta
  • sea bass
  • TROUT ONCORHYNCHUS-MYKISS
  • MOLECULAR-CLONING
  • EXPRESSION ANALYSIS
  • IL-1-BETA
  • SEQUENCE
  • FISH
  • CARP
  • ALIGNMENT
  • CYTOKINES

Cite this

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title = "Peculiar gene organisation and incomplete splicing of sea bass (Dicentrarchus labrax L.) interleukin-1b.",
abstract = "In this paper we describe the gene organisation of sea bass Dicentrarchus labrax interleukin-lbeta (IL-1beta) and the presence of incompletely spliced forms of this gene. Interestingly, the sea bass IL-1beta gene comprises five exons and four introns, thus being remarkably different from other known teleost and mammalian genes. The sizes of the introns were typically much shorter than in mammals and this feature, together with the loss of two introns, gave a Much smaller gene than in mammals (2.7 vs 6.5-7.0 kb). Tile 5'-end exon-intron organisation seems quite different in the known IL-1beta genes with three exons in human and carp, two in trout and only one in sea bass. The highest percentage of exon identity and similarity is between sea bass exon 4, trout exon 5, carp, mouse, chicken, pig and human exon 6. RT-PCR analysis revealed the presence of two incompletely spliced transcripts. Southern blot analysis suggests the presence of only one copy of IL-1beta gene in sea bass genome. (C) 2003 Elsevier Science Ltd. All rights reserved.",
keywords = "gene organisation, incomplete splicing, interleukin-1 beta, sea bass, TROUT ONCORHYNCHUS-MYKISS, MOLECULAR-CLONING, EXPRESSION ANALYSIS, IL-1-BETA, SEQUENCE, FISH, CARP, ALIGNMENT, CYTOKINES",
author = "Secombes, {Christopher John}",
year = "2003",
doi = "10.1016/S1043-4666(03)00095-4",
language = "English",
volume = "21",
pages = "257--264",
journal = "Cytokine",
issn = "1043-4666",
publisher = "Academic Press Inc.",

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TY - JOUR

T1 - Peculiar gene organisation and incomplete splicing of sea bass (Dicentrarchus labrax L.) interleukin-1b.

AU - Secombes, Christopher John

PY - 2003

Y1 - 2003

N2 - In this paper we describe the gene organisation of sea bass Dicentrarchus labrax interleukin-lbeta (IL-1beta) and the presence of incompletely spliced forms of this gene. Interestingly, the sea bass IL-1beta gene comprises five exons and four introns, thus being remarkably different from other known teleost and mammalian genes. The sizes of the introns were typically much shorter than in mammals and this feature, together with the loss of two introns, gave a Much smaller gene than in mammals (2.7 vs 6.5-7.0 kb). Tile 5'-end exon-intron organisation seems quite different in the known IL-1beta genes with three exons in human and carp, two in trout and only one in sea bass. The highest percentage of exon identity and similarity is between sea bass exon 4, trout exon 5, carp, mouse, chicken, pig and human exon 6. RT-PCR analysis revealed the presence of two incompletely spliced transcripts. Southern blot analysis suggests the presence of only one copy of IL-1beta gene in sea bass genome. (C) 2003 Elsevier Science Ltd. All rights reserved.

AB - In this paper we describe the gene organisation of sea bass Dicentrarchus labrax interleukin-lbeta (IL-1beta) and the presence of incompletely spliced forms of this gene. Interestingly, the sea bass IL-1beta gene comprises five exons and four introns, thus being remarkably different from other known teleost and mammalian genes. The sizes of the introns were typically much shorter than in mammals and this feature, together with the loss of two introns, gave a Much smaller gene than in mammals (2.7 vs 6.5-7.0 kb). Tile 5'-end exon-intron organisation seems quite different in the known IL-1beta genes with three exons in human and carp, two in trout and only one in sea bass. The highest percentage of exon identity and similarity is between sea bass exon 4, trout exon 5, carp, mouse, chicken, pig and human exon 6. RT-PCR analysis revealed the presence of two incompletely spliced transcripts. Southern blot analysis suggests the presence of only one copy of IL-1beta gene in sea bass genome. (C) 2003 Elsevier Science Ltd. All rights reserved.

KW - gene organisation

KW - incomplete splicing

KW - interleukin-1 beta

KW - sea bass

KW - TROUT ONCORHYNCHUS-MYKISS

KW - MOLECULAR-CLONING

KW - EXPRESSION ANALYSIS

KW - IL-1-BETA

KW - SEQUENCE

KW - FISH

KW - CARP

KW - ALIGNMENT

KW - CYTOKINES

U2 - 10.1016/S1043-4666(03)00095-4

DO - 10.1016/S1043-4666(03)00095-4

M3 - Article

VL - 21

SP - 257

EP - 264

JO - Cytokine

JF - Cytokine

SN - 1043-4666

ER -