Peptide Cyclization Catalyzed by Cyanobactin Macrocyclases

Wael E. Houssen

doi:10.1007/978-1-4939-9546-2_11

Peptide Cyclization Catalyzed by Cyanobactin Macrocyclases

Wael E. Houssen

Chemistry

Mansoura University

Research output: Chapter in Book/Report/Conference proceeding › Chapter

4 Citations (Scopus)

Abstract

Cyclic peptides are an emerging class of therapeutics that can modulate targets not amenable to traditional small molecule intervention (e.g., protein–protein interactions). However, N-to-C macrocyclization of peptides is a challenging and often a low yielding chemical transformation. Several macrocyclases from cyanobactin biosynthetic clusters have been used to catalyze this reaction.

This chapter provides practical guidance to the processes of heterologous expression and purification of these enzymes as well as performing in vitro biochemical reactions. Finally, approaches to recover the final product from an enzymatic reaction mixture are also discussed.

Original language	English
Title of host publication	Enzyme-Mediated Ligation Methods
Editors	Timo Nuijens, Marcel Schmidt
Place of Publication	New York
Publisher	Springer Nature
Chapter	11
Pages	193-210
Number of pages	18
ISBN (Electronic)	9781493995462
ISBN (Print)	9781493995455
DOIs	https://doi.org/10.1007/978-1-4939-9546-2_11
Publication status	Published - 2019

Publication series

Name	Methods in Molecular Biology
Publisher	Springer
Volume	2012
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Bibliographical note

I dedicate this chapter to the memory of my colleagues Dr. Andrew Bent (University of St Andrews, UK) and Dr. Mostafa Abdelrahman (University of Aberdeen, UK & Al-Azhar University, Egypt) who died from cancer.

Keywords

Cyanobactins
RiPPs
Macrocyclases
Ribosomal peptides
Cyclic peptides
Patellamides
PCC 7425
PATG
NATURAL-PRODUCTS
DISCOVERY
FAMILY
BIOSYNTHESIS
MACROCYCLIZATION

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Cite this

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abstract = "Cyclic peptides are an emerging class of therapeutics that can modulate targets not amenable to traditional small molecule intervention (e.g., protein–protein interactions). However, N-to-C macrocyclization of peptides is a challenging and often a low yielding chemical transformation. Several macrocyclases from cyanobactin biosynthetic clusters have been used to catalyze this reaction.This chapter provides practical guidance to the processes of heterologous expression and purification of these enzymes as well as performing in vitro biochemical reactions. Finally, approaches to recover the final product from an enzymatic reaction mixture are also discussed.",

keywords = "Cyanobactins, RiPPs, Macrocyclases, Ribosomal peptides, Cyclic peptides, Patellamides, PCC 7425, PATG, NATURAL-PRODUCTS, DISCOVERY, FAMILY, BIOSYNTHESIS, MACROCYCLIZATION",

author = "Houssen, {Wael E.}",

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N2 - Cyclic peptides are an emerging class of therapeutics that can modulate targets not amenable to traditional small molecule intervention (e.g., protein–protein interactions). However, N-to-C macrocyclization of peptides is a challenging and often a low yielding chemical transformation. Several macrocyclases from cyanobactin biosynthetic clusters have been used to catalyze this reaction.This chapter provides practical guidance to the processes of heterologous expression and purification of these enzymes as well as performing in vitro biochemical reactions. Finally, approaches to recover the final product from an enzymatic reaction mixture are also discussed.

AB - Cyclic peptides are an emerging class of therapeutics that can modulate targets not amenable to traditional small molecule intervention (e.g., protein–protein interactions). However, N-to-C macrocyclization of peptides is a challenging and often a low yielding chemical transformation. Several macrocyclases from cyanobactin biosynthetic clusters have been used to catalyze this reaction.This chapter provides practical guidance to the processes of heterologous expression and purification of these enzymes as well as performing in vitro biochemical reactions. Finally, approaches to recover the final product from an enzymatic reaction mixture are also discussed.

KW - Cyanobactins

KW - RiPPs

KW - Macrocyclases

KW - Ribosomal peptides

KW - Cyclic peptides

KW - Patellamides

KW - PCC 7425

KW - PATG

KW - NATURAL-PRODUCTS

KW - DISCOVERY

KW - FAMILY

KW - BIOSYNTHESIS

KW - MACROCYCLIZATION

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UR - http://www.mendeley.com/research/peptide-cyclization-catalyzed-cyanobactin-macrocyclases

U2 - 10.1007/978-1-4939-9546-2_11

DO - 10.1007/978-1-4939-9546-2_11

M3 - Chapter

SN - 9781493995455

T3 - Methods in Molecular Biology

SP - 193

EP - 210

BT - Enzyme-Mediated Ligation Methods

A2 - Nuijens, Timo

A2 - Schmidt, Marcel

PB - Springer Nature

CY - New York

ER -

Peptide Cyclization Catalyzed by Cyanobactin Macrocyclases

Abstract

Publication series

Bibliographical note

Keywords

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