Peptide Cyclization Catalyzed by Cyanobactin Macrocyclases

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

Cyclic peptides are an emerging class of therapeutics that can modulate targets not amenable to traditional small molecule intervention (e.g., protein–protein interactions). However, N-to-C macrocyclization of peptides is a challenging and often a low yielding chemical transformation. Several macrocyclases from cyanobactin biosynthetic clusters have been used to catalyze this reaction.

This chapter provides practical guidance to the processes of heterologous expression and purification of these enzymes as well as performing in vitro biochemical reactions. Finally, approaches to recover the final product from an enzymatic reaction mixture are also discussed.
Original languageEnglish
Title of host publicationEnzyme-Mediated Ligation Methods
EditorsTimo Nuijens, Marcel Schmidt
Place of PublicationNew York
PublisherSpringer Nature
Chapter11
Pages193-210
Number of pages18
ISBN (Electronic)9781493995462
ISBN (Print)9781493995455
DOIs
Publication statusPublished - 2019

Publication series

NameMethods in Molecular Biology
PublisherSpringer
Volume2012
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Cyanobactins
  • RiPPs
  • Macrocyclases
  • Ribosomal peptides
  • Cyclic peptides
  • Patellamides
  • PCC 7425
  • PATG
  • NATURAL-PRODUCTS
  • DISCOVERY
  • FAMILY
  • BIOSYNTHESIS
  • MACROCYCLIZATION

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

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  • Cite this

    Houssen, W. E. (2019). Peptide Cyclization Catalyzed by Cyanobactin Macrocyclases. In T. Nuijens, & M. Schmidt (Eds.), Enzyme-Mediated Ligation Methods (pp. 193-210). (Methods in Molecular Biology; Vol. 2012). Springer Nature. https://doi.org/10.1007/978-1-4939-9546-2_11