Permissive corridor and diffusible gradients direct medial ganglionic eminence cell migration to the neocortex

H. Wichterle, M. Alvarez-Dolado, Lynda Erskine, A. Alvarez-Buylla

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Young neurons born in the media[ ganglionic eminence (MGE) migrate a long distance dorsally, giving rise to several types of interneurons in neocortex. The mechanisms that facilitate selective dorsal dispersion of MGE cells while restricting their movement ventrally into neighboring regions are not known. Using microtransplantation into fetal brain slices and onto dissociated substrate cells on floating filters (spot assay), we demonstrate that ventral forebrain regions neighboring the MGE are nonpermissive for MGE cell migration, whereas the dorsal regions leading to the neocortex are increasingly permissive. Spot assay experiments using filters with different pore sizes indicate that the permissive factors are not diffusible. We also show that MGE cells respond to chemoattractive and inhibitory factors diffusing from the neocortex and ventromedial forebrain, respectively. We propose that the final extent and regional specificity of MGE cell dispersion is largely dictated by contact guidance through a selectively permissive environment, flanked by nonpermissive tissues. in addition, we propose that chemotactic guidance cues superimposed over the permissive corridor facilitate efficient dorsal migration of MGE cells.

Original languageEnglish
Pages (from-to)727-732
Number of pages5
JournalPNAS
Volume100
Issue number2
DOIs
Publication statusPublished - 21 Jan 2003

Keywords

  • neuronal migration
  • axon guidance
  • cortical interneurons
  • mammalian forebrain
  • subventricular zone
  • cerebral-cortex
  • basal forebrain
  • heparan-sulfate
  • olfactory-bulb
  • ROBO receptors

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