Permissive corridor and diffusible gradients direct medial ganglionic eminence cell migration to the neocortex

H. Wichterle, M. Alvarez-Dolado, Lynda Erskine, A. Alvarez-Buylla

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Young neurons born in the media[ ganglionic eminence (MGE) migrate a long distance dorsally, giving rise to several types of interneurons in neocortex. The mechanisms that facilitate selective dorsal dispersion of MGE cells while restricting their movement ventrally into neighboring regions are not known. Using microtransplantation into fetal brain slices and onto dissociated substrate cells on floating filters (spot assay), we demonstrate that ventral forebrain regions neighboring the MGE are nonpermissive for MGE cell migration, whereas the dorsal regions leading to the neocortex are increasingly permissive. Spot assay experiments using filters with different pore sizes indicate that the permissive factors are not diffusible. We also show that MGE cells respond to chemoattractive and inhibitory factors diffusing from the neocortex and ventromedial forebrain, respectively. We propose that the final extent and regional specificity of MGE cell dispersion is largely dictated by contact guidance through a selectively permissive environment, flanked by nonpermissive tissues. in addition, we propose that chemotactic guidance cues superimposed over the permissive corridor facilitate efficient dorsal migration of MGE cells.

Original languageEnglish
Pages (from-to)727-732
Number of pages5
JournalPNAS
Volume100
Issue number2
DOIs
Publication statusPublished - 21 Jan 2003

Keywords

  • neuronal migration
  • axon guidance
  • cortical interneurons
  • mammalian forebrain
  • subventricular zone
  • cerebral-cortex
  • basal forebrain
  • heparan-sulfate
  • olfactory-bulb
  • ROBO receptors

Cite this

Permissive corridor and diffusible gradients direct medial ganglionic eminence cell migration to the neocortex. / Wichterle, H.; Alvarez-Dolado, M.; Erskine, Lynda; Alvarez-Buylla, A.

In: PNAS, Vol. 100, No. 2, 21.01.2003, p. 727-732.

Research output: Contribution to journalArticle

Wichterle, H. ; Alvarez-Dolado, M. ; Erskine, Lynda ; Alvarez-Buylla, A. / Permissive corridor and diffusible gradients direct medial ganglionic eminence cell migration to the neocortex. In: PNAS. 2003 ; Vol. 100, No. 2. pp. 727-732.
@article{9fce738cf7a84b649dba319da33a58a2,
title = "Permissive corridor and diffusible gradients direct medial ganglionic eminence cell migration to the neocortex",
abstract = "Young neurons born in the media[ ganglionic eminence (MGE) migrate a long distance dorsally, giving rise to several types of interneurons in neocortex. The mechanisms that facilitate selective dorsal dispersion of MGE cells while restricting their movement ventrally into neighboring regions are not known. Using microtransplantation into fetal brain slices and onto dissociated substrate cells on floating filters (spot assay), we demonstrate that ventral forebrain regions neighboring the MGE are nonpermissive for MGE cell migration, whereas the dorsal regions leading to the neocortex are increasingly permissive. Spot assay experiments using filters with different pore sizes indicate that the permissive factors are not diffusible. We also show that MGE cells respond to chemoattractive and inhibitory factors diffusing from the neocortex and ventromedial forebrain, respectively. We propose that the final extent and regional specificity of MGE cell dispersion is largely dictated by contact guidance through a selectively permissive environment, flanked by nonpermissive tissues. in addition, we propose that chemotactic guidance cues superimposed over the permissive corridor facilitate efficient dorsal migration of MGE cells.",
keywords = "neuronal migration, axon guidance, cortical interneurons, mammalian forebrain, subventricular zone, cerebral-cortex, basal forebrain, heparan-sulfate, olfactory-bulb, ROBO receptors",
author = "H. Wichterle and M. Alvarez-Dolado and Lynda Erskine and A. Alvarez-Buylla",
year = "2003",
month = "1",
day = "21",
doi = "10.1073/PNAS.242721899",
language = "English",
volume = "100",
pages = "727--732",
journal = "PNAS",
issn = "0027-8424",
publisher = "NATL ACAD SCIENCES",
number = "2",

}

TY - JOUR

T1 - Permissive corridor and diffusible gradients direct medial ganglionic eminence cell migration to the neocortex

AU - Wichterle, H.

AU - Alvarez-Dolado, M.

AU - Erskine, Lynda

AU - Alvarez-Buylla, A.

PY - 2003/1/21

Y1 - 2003/1/21

N2 - Young neurons born in the media[ ganglionic eminence (MGE) migrate a long distance dorsally, giving rise to several types of interneurons in neocortex. The mechanisms that facilitate selective dorsal dispersion of MGE cells while restricting their movement ventrally into neighboring regions are not known. Using microtransplantation into fetal brain slices and onto dissociated substrate cells on floating filters (spot assay), we demonstrate that ventral forebrain regions neighboring the MGE are nonpermissive for MGE cell migration, whereas the dorsal regions leading to the neocortex are increasingly permissive. Spot assay experiments using filters with different pore sizes indicate that the permissive factors are not diffusible. We also show that MGE cells respond to chemoattractive and inhibitory factors diffusing from the neocortex and ventromedial forebrain, respectively. We propose that the final extent and regional specificity of MGE cell dispersion is largely dictated by contact guidance through a selectively permissive environment, flanked by nonpermissive tissues. in addition, we propose that chemotactic guidance cues superimposed over the permissive corridor facilitate efficient dorsal migration of MGE cells.

AB - Young neurons born in the media[ ganglionic eminence (MGE) migrate a long distance dorsally, giving rise to several types of interneurons in neocortex. The mechanisms that facilitate selective dorsal dispersion of MGE cells while restricting their movement ventrally into neighboring regions are not known. Using microtransplantation into fetal brain slices and onto dissociated substrate cells on floating filters (spot assay), we demonstrate that ventral forebrain regions neighboring the MGE are nonpermissive for MGE cell migration, whereas the dorsal regions leading to the neocortex are increasingly permissive. Spot assay experiments using filters with different pore sizes indicate that the permissive factors are not diffusible. We also show that MGE cells respond to chemoattractive and inhibitory factors diffusing from the neocortex and ventromedial forebrain, respectively. We propose that the final extent and regional specificity of MGE cell dispersion is largely dictated by contact guidance through a selectively permissive environment, flanked by nonpermissive tissues. in addition, we propose that chemotactic guidance cues superimposed over the permissive corridor facilitate efficient dorsal migration of MGE cells.

KW - neuronal migration

KW - axon guidance

KW - cortical interneurons

KW - mammalian forebrain

KW - subventricular zone

KW - cerebral-cortex

KW - basal forebrain

KW - heparan-sulfate

KW - olfactory-bulb

KW - ROBO receptors

U2 - 10.1073/PNAS.242721899

DO - 10.1073/PNAS.242721899

M3 - Article

VL - 100

SP - 727

EP - 732

JO - PNAS

JF - PNAS

SN - 0027-8424

IS - 2

ER -