Phagocytosis of apoptotic eosinophils but not neutrophils by bronchial epithelial cells

Darren William Sexton, M. Al-Rabia, Morgan Graeme Blaylock, Garry Michael Walsh

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Background We have previously demonstrated that human bronchial epithelial cells engulf apoptotic eosinophils.

Objectives To compare and contrast the phagocytic capabilities of monocyte-derived macrophage and primary airway epithelial cells for apoptotic granulocytes.

Results Here we compared phagocytosis of human apoptotic eosinophils and neutrophils by small and large airway epithelial cells (SAEC and LAEC) and monocyte-derived macrophages. Confocal microscopy of F-actin staining and scanning and transmission electron microscopy revealed phagocytic cup formation around apoptotic eosinophils by airway epithelial cells (AEC) membranes with evidence of their digestion. Resting and cytokine-stimulated AEC did not recognize and ingest apoptotic neutrophils. The latter were phagocytosed by macrophages that exhibited greater ingestion of and higher capacity for, apoptotic eosinophils over apoptotic neutrophils. Cytochalasin D completely abolished uptake of apoptotic eosinophils by SAEC, LAEC or macrophage monolayers. Ligation of epithelial cell CD44 receptors for 24 h increased phagocytosis of apoptotic eosinophils by SAEC and LAEC with a potency comparable with that of IL-1. Phagocytosis was a specific receptor-mediated process involving integrin- (alphavbeta3, alphavbeta5, CD36), phosphatidylserine receptor- and lectin-dependent mechanisms. No significant differences were observed in avarice for apoptotic eosinophils by SAEC or LAEC either resting, CD44 monoclonal antibodies- or cytokine- stimulated, or in their usage and expression of recognition receptors.

Conclusion These findings further suggest and define an important role for the bronchial epithelium in the selective removal of apoptotic eosinophils from the airways in asthma.

Original languageEnglish
Pages (from-to)1514-1524
Number of pages11
JournalClinical & experimental allergy
Volume34
Issue number10
Early online date10 Sep 2004
DOIs
Publication statusPublished - Oct 2004

Keywords

  • apoptotic eosinophils
  • apoptotic neutrophils
  • epithelial cells
  • macrophage
  • phagocytosis
  • COLONY-STIMULATING FACTOR
  • INFLAMMATORY MACROPHAGES
  • RECOGNITION MECHANISM
  • ALVEOLAR MACROPHAGES
  • IN-VITRO
  • LYMPHOCYTES
  • EXPRESSION
  • ACTIVATION
  • RESOLUTION
  • RECEPTOR

Cite this

Phagocytosis of apoptotic eosinophils but not neutrophils by bronchial epithelial cells. / Sexton, Darren William; Al-Rabia, M.; Blaylock, Morgan Graeme; Walsh, Garry Michael.

In: Clinical & experimental allergy, Vol. 34, No. 10, 10.2004, p. 1514-1524.

Research output: Contribution to journalArticle

Sexton, Darren William ; Al-Rabia, M. ; Blaylock, Morgan Graeme ; Walsh, Garry Michael. / Phagocytosis of apoptotic eosinophils but not neutrophils by bronchial epithelial cells. In: Clinical & experimental allergy. 2004 ; Vol. 34, No. 10. pp. 1514-1524.
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AB - Background We have previously demonstrated that human bronchial epithelial cells engulf apoptotic eosinophils.Objectives To compare and contrast the phagocytic capabilities of monocyte-derived macrophage and primary airway epithelial cells for apoptotic granulocytes.Results Here we compared phagocytosis of human apoptotic eosinophils and neutrophils by small and large airway epithelial cells (SAEC and LAEC) and monocyte-derived macrophages. Confocal microscopy of F-actin staining and scanning and transmission electron microscopy revealed phagocytic cup formation around apoptotic eosinophils by airway epithelial cells (AEC) membranes with evidence of their digestion. Resting and cytokine-stimulated AEC did not recognize and ingest apoptotic neutrophils. The latter were phagocytosed by macrophages that exhibited greater ingestion of and higher capacity for, apoptotic eosinophils over apoptotic neutrophils. Cytochalasin D completely abolished uptake of apoptotic eosinophils by SAEC, LAEC or macrophage monolayers. Ligation of epithelial cell CD44 receptors for 24 h increased phagocytosis of apoptotic eosinophils by SAEC and LAEC with a potency comparable with that of IL-1. Phagocytosis was a specific receptor-mediated process involving integrin- (alphavbeta3, alphavbeta5, CD36), phosphatidylserine receptor- and lectin-dependent mechanisms. No significant differences were observed in avarice for apoptotic eosinophils by SAEC or LAEC either resting, CD44 monoclonal antibodies- or cytokine- stimulated, or in their usage and expression of recognition receptors.Conclusion These findings further suggest and define an important role for the bronchial epithelium in the selective removal of apoptotic eosinophils from the airways in asthma.

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