Phase variation in the phage growth limitation system of Streptomyces coelicolor A3(2)

P Sumby, Margaret Caroline MacHin Smith

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The phase-variable phage growth limitation (Pgl) system of Streptomyces coelicolor A3(2) is an unusual bacteriophage resistance mechanism that confers protection against the temperate phage phiC31 and homoimmune relatives. Pgl is subject to phase variation, and data presented here show that this is at least partially due to expansion and contraction of a polyguanine tract present within the putative adenine-specific DNA methyltransferase gene, pglX. Furthermore, the pglX paralogue SC6G9.02, here renamed pglS, was shown to be able to interfere with the Pgl phenotype, suggesting that PglS could provide an alternative activity to that conferred by PgIX.

Original languageEnglish
Pages (from-to)4558-4563
Number of pages6
JournalJournal of Bacteriology
Issue number15
Publication statusPublished - Aug 2003


  • genetic-characterization
  • PGL system
  • restriction
  • DNA
  • expression
  • sequence
  • protein
  • PHI-C31
  • rearrangements
  • specifities

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