Abstract
The phase-variable phage growth limitation (Pgl) system of Streptomyces coelicolor A3(2) is an unusual bacteriophage resistance mechanism that confers protection against the temperate phage phiC31 and homoimmune relatives. Pgl is subject to phase variation, and data presented here show that this is at least partially due to expansion and contraction of a polyguanine tract present within the putative adenine-specific DNA methyltransferase gene, pglX. Furthermore, the pglX paralogue SC6G9.02, here renamed pglS, was shown to be able to interfere with the Pgl phenotype, suggesting that PglS could provide an alternative activity to that conferred by PgIX.
| Original language | English |
|---|---|
| Pages (from-to) | 4558-4563 |
| Number of pages | 6 |
| Journal | Journal of Bacteriology |
| Volume | 185 |
| Issue number | 15 |
| DOIs | |
| Publication status | Published - Aug 2003 |
Keywords
- genetic-characterization
- PGL system
- restriction
- DNA
- expression
- sequence
- protein
- PHI-C31
- rearrangements
- specifities
Cite this
Phase variation in the phage growth limitation system of Streptomyces coelicolor A3(2). / Sumby, P ; Smith, Margaret Caroline MacHin.
In: Journal of Bacteriology, Vol. 185, No. 15, 08.2003, p. 4558-4563.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Phase variation in the phage growth limitation system of Streptomyces coelicolor A3(2)
AU - Sumby, P
AU - Smith, Margaret Caroline MacHin
PY - 2003/8
Y1 - 2003/8
N2 - The phase-variable phage growth limitation (Pgl) system of Streptomyces coelicolor A3(2) is an unusual bacteriophage resistance mechanism that confers protection against the temperate phage phiC31 and homoimmune relatives. Pgl is subject to phase variation, and data presented here show that this is at least partially due to expansion and contraction of a polyguanine tract present within the putative adenine-specific DNA methyltransferase gene, pglX. Furthermore, the pglX paralogue SC6G9.02, here renamed pglS, was shown to be able to interfere with the Pgl phenotype, suggesting that PglS could provide an alternative activity to that conferred by PgIX.
AB - The phase-variable phage growth limitation (Pgl) system of Streptomyces coelicolor A3(2) is an unusual bacteriophage resistance mechanism that confers protection against the temperate phage phiC31 and homoimmune relatives. Pgl is subject to phase variation, and data presented here show that this is at least partially due to expansion and contraction of a polyguanine tract present within the putative adenine-specific DNA methyltransferase gene, pglX. Furthermore, the pglX paralogue SC6G9.02, here renamed pglS, was shown to be able to interfere with the Pgl phenotype, suggesting that PglS could provide an alternative activity to that conferred by PgIX.
KW - genetic-characterization
KW - PGL system
KW - restriction
KW - DNA
KW - expression
KW - sequence
KW - protein
KW - PHI-C31
KW - rearrangements
KW - specifities
U2 - 10.1128/JB.185.15.4558-4563.2003
DO - 10.1128/JB.185.15.4558-4563.2003
M3 - Article
VL - 185
SP - 4558
EP - 4563
JO - Journal of Bacteriology
JF - Journal of Bacteriology
SN - 0021-9193
IS - 15
ER -