Phenotypic diversity among ruminal isolates of Prevotella ruminicola

proposal of Prevotella brevis sp nov, Prevotella bryantii sp nov, and Prevotella albensis sp nov and redefinition of Prevotella ruminicola

G Avgustin, R J Wallace, H J Flint

Research output: Contribution to journalArticle

136 Citations (Scopus)

Abstract

Selected phenotypic characteristics of isolates of Prevotella ruminicola (formerly Bacteroides ruminicola) were studied in order to establish whether the characteristics of genotypic strain groups established previously on the basis of 16S ribosomal DNA sequences differed systematically. Among strains formerly considered P. ruminicola subsp, brevis, strains related to strain GA33(T) (T = type strain) typically failed to produce carboxymethyl cellulase (CMCase) activity detectable by plate assays and failed to ferment xylose, while strains related to strain B(1)4(T) produced abundant CMCase and fermented xylose. We propose that strains related to GA33(T), which have DNA G + C contents between 45 and 51 mol%, should be assigned to a new species, Prevotella brevis, and that strains related to B(1)4(T), which have DNA G + C contents between 39 and 43 mol%, should be assigned to another new species. Prevotella bryantii. Most of the isolates formerly classified as P. ruminicola subsp, ruminicola strains produced CMCase and had DNA G + C contents between 45 and 51 mol%, and we propose that these organisms should be placed in the redefined species P. ruminicola. A small group of isolates that have lower G + C contents are assigned to another new species, Prevotella albensis. Most P. brevis and P. byrantii strains produced abundant extracellular DNase activity. Proteinase activities (as determined by [C-14]casein hydrolysis) varied widely between strains, and P. brevis strains exhibited the highest mean activity. All strains produced dipeptidyl peptidase activity, but the relative activities against different peptide substrates exhibited by P. bryantii, P. albensis, and P. brevis differed systematically. The phenotypic differences among the newly defined species suggest that they may occupy distinct niches within the rumen ecosystem.

Original languageEnglish
Pages (from-to)284-288
Number of pages5
JournalInternational journal of systematic bacteriology
Volume47
Issue number2
DOIs
Publication statusPublished - Apr 1997

Keywords

  • bacteroides-ruminicola
  • genus bacteroides
  • rumen bacteria
  • sheep rumen
  • strains
  • microorganisms
  • enumeration
  • pigs

Cite this

@article{2e2b97f5e0f34995929732d82bd89663,
title = "Phenotypic diversity among ruminal isolates of Prevotella ruminicola: proposal of Prevotella brevis sp nov, Prevotella bryantii sp nov, and Prevotella albensis sp nov and redefinition of Prevotella ruminicola",
abstract = "Selected phenotypic characteristics of isolates of Prevotella ruminicola (formerly Bacteroides ruminicola) were studied in order to establish whether the characteristics of genotypic strain groups established previously on the basis of 16S ribosomal DNA sequences differed systematically. Among strains formerly considered P. ruminicola subsp, brevis, strains related to strain GA33(T) (T = type strain) typically failed to produce carboxymethyl cellulase (CMCase) activity detectable by plate assays and failed to ferment xylose, while strains related to strain B(1)4(T) produced abundant CMCase and fermented xylose. We propose that strains related to GA33(T), which have DNA G + C contents between 45 and 51 mol{\%}, should be assigned to a new species, Prevotella brevis, and that strains related to B(1)4(T), which have DNA G + C contents between 39 and 43 mol{\%}, should be assigned to another new species. Prevotella bryantii. Most of the isolates formerly classified as P. ruminicola subsp, ruminicola strains produced CMCase and had DNA G + C contents between 45 and 51 mol{\%}, and we propose that these organisms should be placed in the redefined species P. ruminicola. A small group of isolates that have lower G + C contents are assigned to another new species, Prevotella albensis. Most P. brevis and P. byrantii strains produced abundant extracellular DNase activity. Proteinase activities (as determined by [C-14]casein hydrolysis) varied widely between strains, and P. brevis strains exhibited the highest mean activity. All strains produced dipeptidyl peptidase activity, but the relative activities against different peptide substrates exhibited by P. bryantii, P. albensis, and P. brevis differed systematically. The phenotypic differences among the newly defined species suggest that they may occupy distinct niches within the rumen ecosystem.",
keywords = "bacteroides-ruminicola, genus bacteroides, rumen bacteria, sheep rumen, strains, microorganisms, enumeration, pigs",
author = "G Avgustin and Wallace, {R J} and Flint, {H J}",
year = "1997",
month = "4",
doi = "10.1099/00207713-47-2-284",
language = "English",
volume = "47",
pages = "284--288",
journal = "International journal of systematic bacteriology",
issn = "0020-7713",
publisher = "Iowa State University Press",
number = "2",

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TY - JOUR

T1 - Phenotypic diversity among ruminal isolates of Prevotella ruminicola

T2 - proposal of Prevotella brevis sp nov, Prevotella bryantii sp nov, and Prevotella albensis sp nov and redefinition of Prevotella ruminicola

AU - Avgustin, G

AU - Wallace, R J

AU - Flint, H J

PY - 1997/4

Y1 - 1997/4

N2 - Selected phenotypic characteristics of isolates of Prevotella ruminicola (formerly Bacteroides ruminicola) were studied in order to establish whether the characteristics of genotypic strain groups established previously on the basis of 16S ribosomal DNA sequences differed systematically. Among strains formerly considered P. ruminicola subsp, brevis, strains related to strain GA33(T) (T = type strain) typically failed to produce carboxymethyl cellulase (CMCase) activity detectable by plate assays and failed to ferment xylose, while strains related to strain B(1)4(T) produced abundant CMCase and fermented xylose. We propose that strains related to GA33(T), which have DNA G + C contents between 45 and 51 mol%, should be assigned to a new species, Prevotella brevis, and that strains related to B(1)4(T), which have DNA G + C contents between 39 and 43 mol%, should be assigned to another new species. Prevotella bryantii. Most of the isolates formerly classified as P. ruminicola subsp, ruminicola strains produced CMCase and had DNA G + C contents between 45 and 51 mol%, and we propose that these organisms should be placed in the redefined species P. ruminicola. A small group of isolates that have lower G + C contents are assigned to another new species, Prevotella albensis. Most P. brevis and P. byrantii strains produced abundant extracellular DNase activity. Proteinase activities (as determined by [C-14]casein hydrolysis) varied widely between strains, and P. brevis strains exhibited the highest mean activity. All strains produced dipeptidyl peptidase activity, but the relative activities against different peptide substrates exhibited by P. bryantii, P. albensis, and P. brevis differed systematically. The phenotypic differences among the newly defined species suggest that they may occupy distinct niches within the rumen ecosystem.

AB - Selected phenotypic characteristics of isolates of Prevotella ruminicola (formerly Bacteroides ruminicola) were studied in order to establish whether the characteristics of genotypic strain groups established previously on the basis of 16S ribosomal DNA sequences differed systematically. Among strains formerly considered P. ruminicola subsp, brevis, strains related to strain GA33(T) (T = type strain) typically failed to produce carboxymethyl cellulase (CMCase) activity detectable by plate assays and failed to ferment xylose, while strains related to strain B(1)4(T) produced abundant CMCase and fermented xylose. We propose that strains related to GA33(T), which have DNA G + C contents between 45 and 51 mol%, should be assigned to a new species, Prevotella brevis, and that strains related to B(1)4(T), which have DNA G + C contents between 39 and 43 mol%, should be assigned to another new species. Prevotella bryantii. Most of the isolates formerly classified as P. ruminicola subsp, ruminicola strains produced CMCase and had DNA G + C contents between 45 and 51 mol%, and we propose that these organisms should be placed in the redefined species P. ruminicola. A small group of isolates that have lower G + C contents are assigned to another new species, Prevotella albensis. Most P. brevis and P. byrantii strains produced abundant extracellular DNase activity. Proteinase activities (as determined by [C-14]casein hydrolysis) varied widely between strains, and P. brevis strains exhibited the highest mean activity. All strains produced dipeptidyl peptidase activity, but the relative activities against different peptide substrates exhibited by P. bryantii, P. albensis, and P. brevis differed systematically. The phenotypic differences among the newly defined species suggest that they may occupy distinct niches within the rumen ecosystem.

KW - bacteroides-ruminicola

KW - genus bacteroides

KW - rumen bacteria

KW - sheep rumen

KW - strains

KW - microorganisms

KW - enumeration

KW - pigs

U2 - 10.1099/00207713-47-2-284

DO - 10.1099/00207713-47-2-284

M3 - Article

VL - 47

SP - 284

EP - 288

JO - International journal of systematic bacteriology

JF - International journal of systematic bacteriology

SN - 0020-7713

IS - 2

ER -