Phosphatidylinositol 3-kinase and xanthine oxidase regulate nitric oxide and reactive oxygen species productions by apoptotic lymphocyte microparticles in endothelial cells

H. Ahmed Mostefai, Abdelali Agouni, Nunzia Carusio, M Letizia Mastronardi, Christophe Heymes, Daniel Henrion, Ramaroson Andriantsitohaina, M. Carmen Martinez

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Microparticles (MPs) are membrane vesicles released during cell activation and apoptosis. We have previously shown that MPs from apoptotic T cells induce endothelial dysfunction, but the mechanisms implicated are not completely elucidated. In this study, we dissect the pathways involved in endothelial cells with respect to both NO and reactive oxygen species (ROS). Incubation of endothelial cells with MPs decreased NO production that was associated with overexpression and phosphorylation of endothelial NO synthase (eNOS). Also, MPs enhanced expression of caveolin-1 and decreased its phosphorylation. Microparticles enhanced ROS by a mechanism sensitive to xanthine oxidase and P-IkappaBalpha inhibitors. PI3K inhibition reduced the effects of MPs on eNOS, but not on caveolin-1, whereas it enhanced the effects of MPs on ROS production. Microparticles stimulated ERK1/2 phosphorylation via a PI3K-depedent mechanism. Inhibition of MEK reversed eNOS phosphorylation but had no effect on ROS production induced by MPs. In vivo injection of MPs in mice impaired endothelial function. In summary, MPs activate pathways related to NO and ROS productions through PI3K, xanthine oxidase, and NF-kappaB pathways. These data underscore the pleiotropic effects of MPs on NO and ROS, leading to an increase oxidative stress that may account for the deleterious effects of MPs on endothelial function.
Original languageEnglish
Pages (from-to)5028-5035
Number of pages8
JournalThe Journal of Immunology
Volume180
Issue number7
Publication statusPublished - 1 Apr 2008

Fingerprint

Phosphatidylinositol 3-Kinase
Xanthine Oxidase
Reactive Oxygen Species
Nitric Oxide
Endothelial Cells
Lymphocytes
Phosphatidylinositol 3-Kinases
Phosphorylation
Nitric Oxide Synthase
Caveolin 1
NF-kappa B
Mitogen-Activated Protein Kinase Kinases
Oxidative Stress
Apoptosis
T-Lymphocytes
Injections
Membranes

Keywords

  • 1-Phosphatidylinositol 3-Kinase
  • Animals
  • Aorta
  • Apoptosis
  • Cell Line
  • Endothelial Cells
  • Humans
  • Lymphocytes
  • MAP Kinase Signaling System
  • Mice
  • Mitogen-Activated Protein Kinases
  • NF-kappa B
  • Nitric Oxide
  • Reactive Oxygen Species
  • Xanthine Oxidase

Cite this

Mostefai, H. A., Agouni, A., Carusio, N., Mastronardi, M. L., Heymes, C., Henrion, D., ... Martinez, M. C. (2008). Phosphatidylinositol 3-kinase and xanthine oxidase regulate nitric oxide and reactive oxygen species productions by apoptotic lymphocyte microparticles in endothelial cells. The Journal of Immunology, 180(7), 5028-5035.

Phosphatidylinositol 3-kinase and xanthine oxidase regulate nitric oxide and reactive oxygen species productions by apoptotic lymphocyte microparticles in endothelial cells. / Mostefai, H. Ahmed; Agouni, Abdelali; Carusio, Nunzia; Mastronardi, M Letizia; Heymes, Christophe; Henrion, Daniel; Andriantsitohaina, Ramaroson; Martinez, M. Carmen.

In: The Journal of Immunology, Vol. 180, No. 7, 01.04.2008, p. 5028-5035.

Research output: Contribution to journalArticle

Mostefai, HA, Agouni, A, Carusio, N, Mastronardi, ML, Heymes, C, Henrion, D, Andriantsitohaina, R & Martinez, MC 2008, 'Phosphatidylinositol 3-kinase and xanthine oxidase regulate nitric oxide and reactive oxygen species productions by apoptotic lymphocyte microparticles in endothelial cells', The Journal of Immunology, vol. 180, no. 7, pp. 5028-5035.
Mostefai, H. Ahmed ; Agouni, Abdelali ; Carusio, Nunzia ; Mastronardi, M Letizia ; Heymes, Christophe ; Henrion, Daniel ; Andriantsitohaina, Ramaroson ; Martinez, M. Carmen. / Phosphatidylinositol 3-kinase and xanthine oxidase regulate nitric oxide and reactive oxygen species productions by apoptotic lymphocyte microparticles in endothelial cells. In: The Journal of Immunology. 2008 ; Vol. 180, No. 7. pp. 5028-5035.
@article{2c92b9b1dd7f40bc92c3b7575d6e5256,
title = "Phosphatidylinositol 3-kinase and xanthine oxidase regulate nitric oxide and reactive oxygen species productions by apoptotic lymphocyte microparticles in endothelial cells",
abstract = "Microparticles (MPs) are membrane vesicles released during cell activation and apoptosis. We have previously shown that MPs from apoptotic T cells induce endothelial dysfunction, but the mechanisms implicated are not completely elucidated. In this study, we dissect the pathways involved in endothelial cells with respect to both NO and reactive oxygen species (ROS). Incubation of endothelial cells with MPs decreased NO production that was associated with overexpression and phosphorylation of endothelial NO synthase (eNOS). Also, MPs enhanced expression of caveolin-1 and decreased its phosphorylation. Microparticles enhanced ROS by a mechanism sensitive to xanthine oxidase and P-IkappaBalpha inhibitors. PI3K inhibition reduced the effects of MPs on eNOS, but not on caveolin-1, whereas it enhanced the effects of MPs on ROS production. Microparticles stimulated ERK1/2 phosphorylation via a PI3K-depedent mechanism. Inhibition of MEK reversed eNOS phosphorylation but had no effect on ROS production induced by MPs. In vivo injection of MPs in mice impaired endothelial function. In summary, MPs activate pathways related to NO and ROS productions through PI3K, xanthine oxidase, and NF-kappaB pathways. These data underscore the pleiotropic effects of MPs on NO and ROS, leading to an increase oxidative stress that may account for the deleterious effects of MPs on endothelial function.",
keywords = "1-Phosphatidylinositol 3-Kinase, Animals, Aorta, Apoptosis, Cell Line, Endothelial Cells, Humans, Lymphocytes, MAP Kinase Signaling System, Mice, Mitogen-Activated Protein Kinases, NF-kappa B, Nitric Oxide, Reactive Oxygen Species, Xanthine Oxidase",
author = "Mostefai, {H. Ahmed} and Abdelali Agouni and Nunzia Carusio and Mastronardi, {M Letizia} and Christophe Heymes and Daniel Henrion and Ramaroson Andriantsitohaina and Martinez, {M. Carmen}",
year = "2008",
month = "4",
day = "1",
language = "English",
volume = "180",
pages = "5028--5035",
journal = "The Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "7",

}

TY - JOUR

T1 - Phosphatidylinositol 3-kinase and xanthine oxidase regulate nitric oxide and reactive oxygen species productions by apoptotic lymphocyte microparticles in endothelial cells

AU - Mostefai, H. Ahmed

AU - Agouni, Abdelali

AU - Carusio, Nunzia

AU - Mastronardi, M Letizia

AU - Heymes, Christophe

AU - Henrion, Daniel

AU - Andriantsitohaina, Ramaroson

AU - Martinez, M. Carmen

PY - 2008/4/1

Y1 - 2008/4/1

N2 - Microparticles (MPs) are membrane vesicles released during cell activation and apoptosis. We have previously shown that MPs from apoptotic T cells induce endothelial dysfunction, but the mechanisms implicated are not completely elucidated. In this study, we dissect the pathways involved in endothelial cells with respect to both NO and reactive oxygen species (ROS). Incubation of endothelial cells with MPs decreased NO production that was associated with overexpression and phosphorylation of endothelial NO synthase (eNOS). Also, MPs enhanced expression of caveolin-1 and decreased its phosphorylation. Microparticles enhanced ROS by a mechanism sensitive to xanthine oxidase and P-IkappaBalpha inhibitors. PI3K inhibition reduced the effects of MPs on eNOS, but not on caveolin-1, whereas it enhanced the effects of MPs on ROS production. Microparticles stimulated ERK1/2 phosphorylation via a PI3K-depedent mechanism. Inhibition of MEK reversed eNOS phosphorylation but had no effect on ROS production induced by MPs. In vivo injection of MPs in mice impaired endothelial function. In summary, MPs activate pathways related to NO and ROS productions through PI3K, xanthine oxidase, and NF-kappaB pathways. These data underscore the pleiotropic effects of MPs on NO and ROS, leading to an increase oxidative stress that may account for the deleterious effects of MPs on endothelial function.

AB - Microparticles (MPs) are membrane vesicles released during cell activation and apoptosis. We have previously shown that MPs from apoptotic T cells induce endothelial dysfunction, but the mechanisms implicated are not completely elucidated. In this study, we dissect the pathways involved in endothelial cells with respect to both NO and reactive oxygen species (ROS). Incubation of endothelial cells with MPs decreased NO production that was associated with overexpression and phosphorylation of endothelial NO synthase (eNOS). Also, MPs enhanced expression of caveolin-1 and decreased its phosphorylation. Microparticles enhanced ROS by a mechanism sensitive to xanthine oxidase and P-IkappaBalpha inhibitors. PI3K inhibition reduced the effects of MPs on eNOS, but not on caveolin-1, whereas it enhanced the effects of MPs on ROS production. Microparticles stimulated ERK1/2 phosphorylation via a PI3K-depedent mechanism. Inhibition of MEK reversed eNOS phosphorylation but had no effect on ROS production induced by MPs. In vivo injection of MPs in mice impaired endothelial function. In summary, MPs activate pathways related to NO and ROS productions through PI3K, xanthine oxidase, and NF-kappaB pathways. These data underscore the pleiotropic effects of MPs on NO and ROS, leading to an increase oxidative stress that may account for the deleterious effects of MPs on endothelial function.

KW - 1-Phosphatidylinositol 3-Kinase

KW - Animals

KW - Aorta

KW - Apoptosis

KW - Cell Line

KW - Endothelial Cells

KW - Humans

KW - Lymphocytes

KW - MAP Kinase Signaling System

KW - Mice

KW - Mitogen-Activated Protein Kinases

KW - NF-kappa B

KW - Nitric Oxide

KW - Reactive Oxygen Species

KW - Xanthine Oxidase

M3 - Article

VL - 180

SP - 5028

EP - 5035

JO - The Journal of Immunology

JF - The Journal of Immunology

SN - 0022-1767

IS - 7

ER -