Phosphocholine content of rat sarcoma cells in the presence and absence of serum,

Timothy Andrew Davies Smith, S A Eccles, G Box, Jenny Titley, Martin Leach, Ralph McReady

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Abstract

Rat sarcoma cells were grown in vitro in tissue culture medium (DMEM) supplemented with 10% foetal calf serum for 5 to 8 days followed by serum withdrawal to produce populations of cells with a variety of cell cycle distributions. Phosphocholine (PCho) and choline content and S + G2 fraction were determined. The phosphocholine content of faster growing populations of serum supplemented cells was higher than the slower growing populations. Choline content was not consistently associated with S + G2 fraction. Serum deprivation was accompanied by a decrease in S + G2 fraction after 24 hours but even after 48 hours PCho content was only slightly decreased.
Original languageEnglish
Pages (from-to)1389-1392
Number of pages4
JournalAnticancer Research
Volume16
Issue number3B
Publication statusPublished - 1996

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Smith, T. A. D., Eccles, S. A., Box, G., Titley, J., Leach, M., & McReady, R. (1996). Phosphocholine content of rat sarcoma cells in the presence and absence of serum, Anticancer Research, 16(3B), 1389-1392.