Photoperiod-dependent regulation of carboxypeptidase E affects selective processing of neuropeptides in the seasonal Siberian hamster (Phodopus sungorus)

Michael Helwig, Annika Herwig, Gerhard Heldmaier, Perry Barrett, Julian G Mercer, Martin Klingenspor

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The production of bioactive peptides from biologically inactive precursors involves extensive post-translational processing including the enzymatic cleavage by proteolytic peptidases. Endoproteolytic prohormone-convertases initially cleave the precursors of many neuropeptides at specific amino acid sequences to generate intermediates with basic amino acid extensions on their C-termini. Afterwards, the related exopeptidases, carboxypeptidases D and E (CPD, CPE), are responsible for removing these amino acids before the peptides achieve biological activity. We investigated the effect of photoperiod on the processing of the neuropeptide precursor pro-opiomelanocortin (POMC) and its derived neuropeptides, alpha- melanocyte stimulating hormone (a-MSH) and ß-endorphin (ß-END), within the hypothalamus of the seasonal Siberian hamster (Phodopus sungorus). We thus compared hypothalamic distribution of CPD, CPE, a-MSH and ß-END using immunohistochemistry and measured enzyme activity of CPE and concentrations of C-terminally cleaved a-MSH in short day (SD, 8h/16h light/dark) and long day (LD, 16h/8h light/dark) acclimatised hamsters. Increased immunoreactivity (-ir) of CPE as well as higher CPE-activity were observed in SD. This increase was accompanied by more ß-END-ir cells and substantially higher levels of C- terminally cleaved a-MSH, as determined by RIA. Our results suggest that exoproteolytic cleavage of POMC-derived neuropeptides is tightly regulated by photoperiod in the Siberian hamster. Higher levels of biological active a-MSH- and ß-END in SD are consistent with the hypothesis that posttranslational processing is a key event in the regulation of seasonal energy balance. © 2012 The Authors. Journal of Neuroendocrinology © 2012 British Society forNeuroendocrinology.
Original languageEnglish
Pages (from-to)190-197
Number of pages8
JournalJournal of Neuroendocrinology
Volume25
Issue number2
Early online date24 Jan 2013
DOIs
Publication statusPublished - Feb 2013

Fingerprint

Carboxypeptidase H
Phodopus
alpha-MSH
Photoperiod
Neuropeptides
Pro-Opiomelanocortin
Exopeptidases
Neuroendocrinology
Proprotein Convertases
Endorphins
Light
Basic Amino Acids
Peptides
Cricetinae
Hypothalamus
Amino Acid Sequence
Peptide Hydrolases
Immunohistochemistry
Amino Acids
Enzymes

Keywords

  • pro-opiomelanocortin
  • neuropeptides
  • carboxypetidases
  • hypothalamus
  • photoperiod

Cite this

Photoperiod-dependent regulation of carboxypeptidase E affects selective processing of neuropeptides in the seasonal Siberian hamster (Phodopus sungorus). / Helwig, Michael; Herwig, Annika; Heldmaier, Gerhard; Barrett, Perry; Mercer, Julian G; Klingenspor, Martin.

In: Journal of Neuroendocrinology, Vol. 25, No. 2, 02.2013, p. 190-197.

Research output: Contribution to journalArticle

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T1 - Photoperiod-dependent regulation of carboxypeptidase E affects selective processing of neuropeptides in the seasonal Siberian hamster (Phodopus sungorus)

AU - Helwig, Michael

AU - Herwig, Annika

AU - Heldmaier, Gerhard

AU - Barrett, Perry

AU - Mercer, Julian G

AU - Klingenspor, Martin

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N2 - The production of bioactive peptides from biologically inactive precursors involves extensive post-translational processing including the enzymatic cleavage by proteolytic peptidases. Endoproteolytic prohormone-convertases initially cleave the precursors of many neuropeptides at specific amino acid sequences to generate intermediates with basic amino acid extensions on their C-termini. Afterwards, the related exopeptidases, carboxypeptidases D and E (CPD, CPE), are responsible for removing these amino acids before the peptides achieve biological activity. We investigated the effect of photoperiod on the processing of the neuropeptide precursor pro-opiomelanocortin (POMC) and its derived neuropeptides, alpha- melanocyte stimulating hormone (a-MSH) and ß-endorphin (ß-END), within the hypothalamus of the seasonal Siberian hamster (Phodopus sungorus). We thus compared hypothalamic distribution of CPD, CPE, a-MSH and ß-END using immunohistochemistry and measured enzyme activity of CPE and concentrations of C-terminally cleaved a-MSH in short day (SD, 8h/16h light/dark) and long day (LD, 16h/8h light/dark) acclimatised hamsters. Increased immunoreactivity (-ir) of CPE as well as higher CPE-activity were observed in SD. This increase was accompanied by more ß-END-ir cells and substantially higher levels of C- terminally cleaved a-MSH, as determined by RIA. Our results suggest that exoproteolytic cleavage of POMC-derived neuropeptides is tightly regulated by photoperiod in the Siberian hamster. Higher levels of biological active a-MSH- and ß-END in SD are consistent with the hypothesis that posttranslational processing is a key event in the regulation of seasonal energy balance. © 2012 The Authors. Journal of Neuroendocrinology © 2012 British Society forNeuroendocrinology.

AB - The production of bioactive peptides from biologically inactive precursors involves extensive post-translational processing including the enzymatic cleavage by proteolytic peptidases. Endoproteolytic prohormone-convertases initially cleave the precursors of many neuropeptides at specific amino acid sequences to generate intermediates with basic amino acid extensions on their C-termini. Afterwards, the related exopeptidases, carboxypeptidases D and E (CPD, CPE), are responsible for removing these amino acids before the peptides achieve biological activity. We investigated the effect of photoperiod on the processing of the neuropeptide precursor pro-opiomelanocortin (POMC) and its derived neuropeptides, alpha- melanocyte stimulating hormone (a-MSH) and ß-endorphin (ß-END), within the hypothalamus of the seasonal Siberian hamster (Phodopus sungorus). We thus compared hypothalamic distribution of CPD, CPE, a-MSH and ß-END using immunohistochemistry and measured enzyme activity of CPE and concentrations of C-terminally cleaved a-MSH in short day (SD, 8h/16h light/dark) and long day (LD, 16h/8h light/dark) acclimatised hamsters. Increased immunoreactivity (-ir) of CPE as well as higher CPE-activity were observed in SD. This increase was accompanied by more ß-END-ir cells and substantially higher levels of C- terminally cleaved a-MSH, as determined by RIA. Our results suggest that exoproteolytic cleavage of POMC-derived neuropeptides is tightly regulated by photoperiod in the Siberian hamster. Higher levels of biological active a-MSH- and ß-END in SD are consistent with the hypothesis that posttranslational processing is a key event in the regulation of seasonal energy balance. © 2012 The Authors. Journal of Neuroendocrinology © 2012 British Society forNeuroendocrinology.

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KW - carboxypetidases

KW - hypothalamus

KW - photoperiod

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DO - 10.1111/j.1365-2826.2012.02384.x

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EP - 197

JO - Journal of Neuroendocrinology

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