Photoperiod regulates arcuate nucleus POMC, AGRP, and leptin receptor mRNA in Siberian hamster hypothalamus

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Abstract

Siberian hamsters decreased body weight by 30% during 18 wk in short day (SD) vs. long day (LD) controls. Subsequent imposed food deprivation (FD; 24 h) caused a further 10% decrease. In the hypothalamic arcuate nucleus (ARC), SDs reduced proopiomelanocortin (POMC) gene expression and agouti-related protein (AGRP) mRNA was elevated, changes that summate to reduced catabolic drive through the melanocortin receptors. There was no effect of photoperiod on neuropeptide Y (NPY), melanin concentrating hormone, orexin, or corticotropin-releasing factor mRNAs. Superimposed FD increased AGRP gene expression and caused a localized elevation of NPY mRNA in the ARC. Both adipose tissue leptin and ARC leptin receptor (OB-Rb) mRNAs were downregulated in SDs, whereas FD increased OB-Rb gene expression. Thus OB-Rb mRNA is differentially regulated by acute and chronic changes in plasma leptin in this species. In a separate experiment in LDs, AGRP gene expression was increased by 24 or 48 h FD, whereas POMC mRNA was downregulated in the caudal ARC. AGRP and NPY mRNAs were extensively coexpressed in the ARC, and their differential regulation by photoperiod and FD is suggestive of transcript-specific regulation at the level of individual neurons.
Original languageEnglish
Pages (from-to)R271-81
Number of pages11
JournalAmerican Journal of Physiology-Regulatory Integrative and Comparative Physiology
Volume278
Issue number1
Publication statusPublished - 1 Jan 2000

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Agouti-Related Protein
Phodopus
Leptin Receptors
Pro-Opiomelanocortin
Arcuate Nucleus of Hypothalamus
Photoperiod
Hypothalamus
Messenger RNA
Neuropeptide Y
Gene Expression
Leptin
Down-Regulation
Melanocortin Receptors
Retinoblastoma Genes
Food Deprivation
Corticotropin-Releasing Hormone
Adipose Tissue
Body Weight
Neurons

Keywords

  • Adipose Tissue
  • Agouti-Related Protein
  • Animals
  • Arcuate Nucleus
  • Carrier Proteins
  • Cricetinae
  • Down-Regulation
  • Eating
  • Food Deprivation
  • Gene Expression
  • Intercellular Signaling Peptides and Proteins
  • Male
  • Neuropeptide Y
  • Phodopus
  • Photoperiod
  • Pro-Opiomelanocortin
  • Proteins
  • RNA, Messenger
  • Receptors, Cell Surface
  • Receptors, Leptin
  • Time Factors
  • Tissue Distribution
  • Orexins
  • Melanin-Concentrating Hormone

Cite this

@article{5f988bb8096d4fc0889f5abe45941a6c,
title = "Photoperiod regulates arcuate nucleus POMC, AGRP, and leptin receptor mRNA in Siberian hamster hypothalamus",
abstract = "Siberian hamsters decreased body weight by 30{\%} during 18 wk in short day (SD) vs. long day (LD) controls. Subsequent imposed food deprivation (FD; 24 h) caused a further 10{\%} decrease. In the hypothalamic arcuate nucleus (ARC), SDs reduced proopiomelanocortin (POMC) gene expression and agouti-related protein (AGRP) mRNA was elevated, changes that summate to reduced catabolic drive through the melanocortin receptors. There was no effect of photoperiod on neuropeptide Y (NPY), melanin concentrating hormone, orexin, or corticotropin-releasing factor mRNAs. Superimposed FD increased AGRP gene expression and caused a localized elevation of NPY mRNA in the ARC. Both adipose tissue leptin and ARC leptin receptor (OB-Rb) mRNAs were downregulated in SDs, whereas FD increased OB-Rb gene expression. Thus OB-Rb mRNA is differentially regulated by acute and chronic changes in plasma leptin in this species. In a separate experiment in LDs, AGRP gene expression was increased by 24 or 48 h FD, whereas POMC mRNA was downregulated in the caudal ARC. AGRP and NPY mRNAs were extensively coexpressed in the ARC, and their differential regulation by photoperiod and FD is suggestive of transcript-specific regulation at the level of individual neurons.",
keywords = "Adipose Tissue, Agouti-Related Protein, Animals, Arcuate Nucleus, Carrier Proteins, Cricetinae, Down-Regulation, Eating, Food Deprivation, Gene Expression, Intercellular Signaling Peptides and Proteins, Male, Neuropeptide Y, Phodopus, Photoperiod, Pro-Opiomelanocortin, Proteins, RNA, Messenger, Receptors, Cell Surface, Receptors, Leptin, Time Factors, Tissue Distribution, Orexins, Melanin-Concentrating Hormone",
author = "Julian Mercer and Kim-Marie Moar and Alexander Ross and Nigel Hoggard and Morgan, {Peter John}",
year = "2000",
month = "1",
day = "1",
language = "English",
volume = "278",
pages = "R271--81",
journal = "American Journal of Physiology-Regulatory Integrative and Comparative Physiology",
issn = "0363-6119",
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TY - JOUR

T1 - Photoperiod regulates arcuate nucleus POMC, AGRP, and leptin receptor mRNA in Siberian hamster hypothalamus

AU - Mercer, Julian

AU - Moar, Kim-Marie

AU - Ross, Alexander

AU - Hoggard, Nigel

AU - Morgan, Peter John

PY - 2000/1/1

Y1 - 2000/1/1

N2 - Siberian hamsters decreased body weight by 30% during 18 wk in short day (SD) vs. long day (LD) controls. Subsequent imposed food deprivation (FD; 24 h) caused a further 10% decrease. In the hypothalamic arcuate nucleus (ARC), SDs reduced proopiomelanocortin (POMC) gene expression and agouti-related protein (AGRP) mRNA was elevated, changes that summate to reduced catabolic drive through the melanocortin receptors. There was no effect of photoperiod on neuropeptide Y (NPY), melanin concentrating hormone, orexin, or corticotropin-releasing factor mRNAs. Superimposed FD increased AGRP gene expression and caused a localized elevation of NPY mRNA in the ARC. Both adipose tissue leptin and ARC leptin receptor (OB-Rb) mRNAs were downregulated in SDs, whereas FD increased OB-Rb gene expression. Thus OB-Rb mRNA is differentially regulated by acute and chronic changes in plasma leptin in this species. In a separate experiment in LDs, AGRP gene expression was increased by 24 or 48 h FD, whereas POMC mRNA was downregulated in the caudal ARC. AGRP and NPY mRNAs were extensively coexpressed in the ARC, and their differential regulation by photoperiod and FD is suggestive of transcript-specific regulation at the level of individual neurons.

AB - Siberian hamsters decreased body weight by 30% during 18 wk in short day (SD) vs. long day (LD) controls. Subsequent imposed food deprivation (FD; 24 h) caused a further 10% decrease. In the hypothalamic arcuate nucleus (ARC), SDs reduced proopiomelanocortin (POMC) gene expression and agouti-related protein (AGRP) mRNA was elevated, changes that summate to reduced catabolic drive through the melanocortin receptors. There was no effect of photoperiod on neuropeptide Y (NPY), melanin concentrating hormone, orexin, or corticotropin-releasing factor mRNAs. Superimposed FD increased AGRP gene expression and caused a localized elevation of NPY mRNA in the ARC. Both adipose tissue leptin and ARC leptin receptor (OB-Rb) mRNAs were downregulated in SDs, whereas FD increased OB-Rb gene expression. Thus OB-Rb mRNA is differentially regulated by acute and chronic changes in plasma leptin in this species. In a separate experiment in LDs, AGRP gene expression was increased by 24 or 48 h FD, whereas POMC mRNA was downregulated in the caudal ARC. AGRP and NPY mRNAs were extensively coexpressed in the ARC, and their differential regulation by photoperiod and FD is suggestive of transcript-specific regulation at the level of individual neurons.

KW - Adipose Tissue

KW - Agouti-Related Protein

KW - Animals

KW - Arcuate Nucleus

KW - Carrier Proteins

KW - Cricetinae

KW - Down-Regulation

KW - Eating

KW - Food Deprivation

KW - Gene Expression

KW - Intercellular Signaling Peptides and Proteins

KW - Male

KW - Neuropeptide Y

KW - Phodopus

KW - Photoperiod

KW - Pro-Opiomelanocortin

KW - Proteins

KW - RNA, Messenger

KW - Receptors, Cell Surface

KW - Receptors, Leptin

KW - Time Factors

KW - Tissue Distribution

KW - Orexins

KW - Melanin-Concentrating Hormone

M3 - Article

VL - 278

SP - R271-81

JO - American Journal of Physiology-Regulatory Integrative and Comparative Physiology

JF - American Journal of Physiology-Regulatory Integrative and Comparative Physiology

SN - 0363-6119

IS - 1

ER -