Photoperiod Regulates Genes Encoding Melanocortin 3 and Serotonin Receptors and Secretogranins in the Dorsomedial Posterior Arcuate of the Siberian Hamster

K. N. Nilaweera, Z. A. Archer, Gillian Patricia Campbell, Claus-Dieter Mayer, A. Balik, Alexander Ross, Julian Mercer, F. J. P. Ebling, Peter John Morgan, Perry Barrett

Research output: Contribution to journalArticle

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Abstract

The mechanism(s) involved in the regulation of the seasonal-appropriate body weight of the Siberian hamster are currently unknown. We have identified photoperiodically regulated genes including VGF in a sub-region of the arcuate nucleus termed the dorsomedial posterior arcuate (dmpARC). Gene expression changes in this nucleus so far account for a significant number of those reported as photoperiodically regulated and are therefore likely to contribute to seasonal physiological responses of the hamsters. The present study aimed to identify additional genes expressed in the dmpARC regulated by photoperiod that could be involved in regulating the activity of this nucleus with respect to seasonal physiology of the Siberian hamster. Using laser capture microdissection coupled with a microarray analysis and a candidate gene approach, we have identified several photoperiodically regulated genes in the dmpARC that are known to have roles in secretory and intracellular signalling pathways. These include secretogranin (sg) III and SgVI (secretory pathway), melanocortin 3 receptor (MC3-R) and serotonin (5-HT) receptors 2A and 7 (signalling pathway), all of which increase in expression under a short photoperiod. The spatial relationship between receptor signalling and potential secretory pathways was investigated by dual in situ hybridisation, which revealed that 5-HT2A and 5-HT7 receptors are expressed in neurones expressing VGF mRNA and that a sub-population (approximately 40%) of these neurones express MC3-R. These gene expression changes in dmpARC neurones may reflect the functional requirement of these neurones for seasonal physiological responses of the hamster.

Original languageEnglish
Pages (from-to)123-131
Number of pages9
JournalJournal of Neuroendocrinology
Volume21
Issue number2
DOIs
Publication statusPublished - Feb 2009

Keywords

  • body weight
  • hypothalamus
  • laser capture
  • microarray
  • body-weight
  • seasonal responses
  • energy-balance
  • granin family
  • expression
  • nucleus
  • VGF
  • reproduction
  • melatonin
  • hormone
  • microarray

Cite this

Photoperiod Regulates Genes Encoding Melanocortin 3 and Serotonin Receptors and Secretogranins in the Dorsomedial Posterior Arcuate of the Siberian Hamster. / Nilaweera, K. N.; Archer, Z. A.; Campbell, Gillian Patricia; Mayer, Claus-Dieter; Balik, A.; Ross, Alexander; Mercer, Julian; Ebling, F. J. P.; Morgan, Peter John; Barrett, Perry.

In: Journal of Neuroendocrinology, Vol. 21, No. 2, 02.2009, p. 123-131.

Research output: Contribution to journalArticle

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abstract = "The mechanism(s) involved in the regulation of the seasonal-appropriate body weight of the Siberian hamster are currently unknown. We have identified photoperiodically regulated genes including VGF in a sub-region of the arcuate nucleus termed the dorsomedial posterior arcuate (dmpARC). Gene expression changes in this nucleus so far account for a significant number of those reported as photoperiodically regulated and are therefore likely to contribute to seasonal physiological responses of the hamsters. The present study aimed to identify additional genes expressed in the dmpARC regulated by photoperiod that could be involved in regulating the activity of this nucleus with respect to seasonal physiology of the Siberian hamster. Using laser capture microdissection coupled with a microarray analysis and a candidate gene approach, we have identified several photoperiodically regulated genes in the dmpARC that are known to have roles in secretory and intracellular signalling pathways. These include secretogranin (sg) III and SgVI (secretory pathway), melanocortin 3 receptor (MC3-R) and serotonin (5-HT) receptors 2A and 7 (signalling pathway), all of which increase in expression under a short photoperiod. The spatial relationship between receptor signalling and potential secretory pathways was investigated by dual in situ hybridisation, which revealed that 5-HT2A and 5-HT7 receptors are expressed in neurones expressing VGF mRNA and that a sub-population (approximately 40{\%}) of these neurones express MC3-R. These gene expression changes in dmpARC neurones may reflect the functional requirement of these neurones for seasonal physiological responses of the hamster.",
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AU - Archer, Z. A.

AU - Campbell, Gillian Patricia

AU - Mayer, Claus-Dieter

AU - Balik, A.

AU - Ross, Alexander

AU - Mercer, Julian

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AU - Morgan, Peter John

AU - Barrett, Perry

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AB - The mechanism(s) involved in the regulation of the seasonal-appropriate body weight of the Siberian hamster are currently unknown. We have identified photoperiodically regulated genes including VGF in a sub-region of the arcuate nucleus termed the dorsomedial posterior arcuate (dmpARC). Gene expression changes in this nucleus so far account for a significant number of those reported as photoperiodically regulated and are therefore likely to contribute to seasonal physiological responses of the hamsters. The present study aimed to identify additional genes expressed in the dmpARC regulated by photoperiod that could be involved in regulating the activity of this nucleus with respect to seasonal physiology of the Siberian hamster. Using laser capture microdissection coupled with a microarray analysis and a candidate gene approach, we have identified several photoperiodically regulated genes in the dmpARC that are known to have roles in secretory and intracellular signalling pathways. These include secretogranin (sg) III and SgVI (secretory pathway), melanocortin 3 receptor (MC3-R) and serotonin (5-HT) receptors 2A and 7 (signalling pathway), all of which increase in expression under a short photoperiod. The spatial relationship between receptor signalling and potential secretory pathways was investigated by dual in situ hybridisation, which revealed that 5-HT2A and 5-HT7 receptors are expressed in neurones expressing VGF mRNA and that a sub-population (approximately 40%) of these neurones express MC3-R. These gene expression changes in dmpARC neurones may reflect the functional requirement of these neurones for seasonal physiological responses of the hamster.

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KW - expression

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KW - VGF

KW - reproduction

KW - melatonin

KW - hormone

KW - microarray

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