Photoperiodic regulation of glycogen metabolism, glycolysis, and glutamine synthesis in tanycytes of the Siberian hamster suggests novel roles of tanycytes in hypothalamic function

Kanishka Nilaweera, Annika Herwig, Matei Bolborea, Gill Campbell, Claus D Mayer, Peter J Morgan, Francis J P Ebling, Perry Barrett

Research output: Contribution to journalArticle

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Abstract

The objective of this study is to investigate the impact of photoperiod on the temporal and spatial expression of genes involved in glucose metabolism in the brain of the seasonal mammal Phodopus sungorus (Siberian hamster). In situ hybridization was performed on brain sections obtained from male hamsters held in long photoperiod (high body weight and developed testes) or short photoperiod (reduced body weight with testicular regression). This analysis revealed upregulation in expression of genes involved in glycogen and glucose metabolism in short photoperiod and localized to the tanycyte layer of the third ventricle. On the basis of these data and a previously identified photoperiod-dependent increase in activity of neighboring hypothalamic neurons, we hypothesized that the observed expression changes may reflect alteration in either metabolic fuel or precursor neurotransmitter supply to surrounding neurons. Gene expression analysis was performed for genes involved in lactate and glutamate transport. This analysis showed that the gene for the lactate transporter MCT2 and glutamate transporter GLAST was decreased in the tanycyte layer in short photoperiod. Expression of mRNA for glutamine synthetase, the final enzyme in the synthesis of the neuronal neurotransmitter precursor, glutamine, was also decreased in short photoperiod. These data suggest a role for tanycytes in modulating glutamate concentrations and neurotransmitter supply in the hypothalamic environment. © 2011 Wiley-Liss, Inc.
Original languageEnglish
Pages (from-to)1695-1705
Number of pages11
JournalGlia
Volume59
Issue number11
DOIs
Publication statusPublished - Nov 2011

Fingerprint

Phodopus
Ependymoglial Cells
Photoperiod
Glycolysis
Glutamine
Glycogen
Neurotransmitter Agents
Gene Expression
Glutamic Acid
Monocarboxylic Acid Transporters
Body Weight
Amino Acid Transport System X-AG
Neurons
Glucose
Glutamate-Ammonia Ligase
Third Ventricle
Brain
Cricetinae
Genes
In Situ Hybridization

Keywords

  • hypothalamus
  • seasonality
  • melatonin
  • monocarboxylate transporter

Cite this

Photoperiodic regulation of glycogen metabolism, glycolysis, and glutamine synthesis in tanycytes of the Siberian hamster suggests novel roles of tanycytes in hypothalamic function. / Nilaweera, Kanishka; Herwig, Annika; Bolborea, Matei; Campbell, Gill; Mayer, Claus D; Morgan, Peter J; Ebling, Francis J P; Barrett, Perry.

In: Glia, Vol. 59, No. 11, 11.2011, p. 1695-1705.

Research output: Contribution to journalArticle

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