Photoperiodic regulation of hypothalamic retinoid signaling

Association of retinoid X receptor gamma with body weight

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

This study reports novel events related to photoperiodic programming of the neuroendocrine hypothalamus. To investigate photoperiod-responsive genes, Siberian hamsters were maintained in long or short photoperiods that generate physiological states of obesity or leanness. Microarray expression analysis first identified CRBP1 as a photoperiod-responsive gene, and then further studies using in situ hybridization and immunocytochemistry revealed that expression levels of several related retinoid-signaling genes were modulated in response to photoperiod changes. Genes of the retinoid-signaling pathway, encoding nuclear receptors (RXR/RAR) and retinoid binding proteins (CRBP1 and CRABP2) are photoperiodically regulated in the dorsal tuberomamillary nucleus (DTM): Their expression is significantly lower in short photoperiods and parallels body weight decreases. Studies in pinealectomized hamsters confirm that the pineal melatonin rhythm is necessary for these seasonal changes, and studies in testosterone-treated hamsters reveal that these changes in gene expression are not the secondary consequence of photoperiod-induced changes in steroid levels. Comparative studies using Syrian hamsters, which show divergent seasonal body weight responses to Siberian hamsters when exposed to short photoperiods, showed a distinct pattern of changes in retinoid gene expression in the DTM in response to a change in photoperiod. We infer that the DTM may be an important integrating center for photoperiodic control of seasonal physiology and suggest that the changes in retinoid X receptor gamma expression may be associated with seasonal changes in body weight and energy metabolism.

Original languageEnglish
Pages (from-to)13-20
Number of pages8
JournalEndocrinology
Volume145
Issue number1
DOIs
Publication statusPublished - Jan 2004

Keywords

  • Siberian hamsters
  • Syrian-hamsters
  • gene-expression
  • food-intake
  • suprachiasmatic nucleus
  • melatonin infusions
  • Djungarian hamsters
  • phodopus-sungorus
  • pars tuberalis
  • induced cycles

Cite this

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title = "Photoperiodic regulation of hypothalamic retinoid signaling: Association of retinoid X receptor gamma with body weight",
abstract = "This study reports novel events related to photoperiodic programming of the neuroendocrine hypothalamus. To investigate photoperiod-responsive genes, Siberian hamsters were maintained in long or short photoperiods that generate physiological states of obesity or leanness. Microarray expression analysis first identified CRBP1 as a photoperiod-responsive gene, and then further studies using in situ hybridization and immunocytochemistry revealed that expression levels of several related retinoid-signaling genes were modulated in response to photoperiod changes. Genes of the retinoid-signaling pathway, encoding nuclear receptors (RXR/RAR) and retinoid binding proteins (CRBP1 and CRABP2) are photoperiodically regulated in the dorsal tuberomamillary nucleus (DTM): Their expression is significantly lower in short photoperiods and parallels body weight decreases. Studies in pinealectomized hamsters confirm that the pineal melatonin rhythm is necessary for these seasonal changes, and studies in testosterone-treated hamsters reveal that these changes in gene expression are not the secondary consequence of photoperiod-induced changes in steroid levels. Comparative studies using Syrian hamsters, which show divergent seasonal body weight responses to Siberian hamsters when exposed to short photoperiods, showed a distinct pattern of changes in retinoid gene expression in the DTM in response to a change in photoperiod. We infer that the DTM may be an important integrating center for photoperiodic control of seasonal physiology and suggest that the changes in retinoid X receptor gamma expression may be associated with seasonal changes in body weight and energy metabolism.",
keywords = "Siberian hamsters, Syrian-hamsters, gene-expression, food-intake, suprachiasmatic nucleus, melatonin infusions, Djungarian hamsters, phodopus-sungorus, pars tuberalis, induced cycles",
author = "Alexander Ross and Webster, {C A} and Julian Mercer and Kim-Marie Moar and Ebling, {F J} and S Schuhler and Perry Barrett and Morgan, {Peter John}",
year = "2004",
month = "1",
doi = "10.1210/en.2003-0838",
language = "English",
volume = "145",
pages = "13--20",
journal = "Endocrinology",
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publisher = "The Endocrine Society",
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TY - JOUR

T1 - Photoperiodic regulation of hypothalamic retinoid signaling

T2 - Association of retinoid X receptor gamma with body weight

AU - Ross, Alexander

AU - Webster, C A

AU - Mercer, Julian

AU - Moar, Kim-Marie

AU - Ebling, F J

AU - Schuhler, S

AU - Barrett, Perry

AU - Morgan, Peter John

PY - 2004/1

Y1 - 2004/1

N2 - This study reports novel events related to photoperiodic programming of the neuroendocrine hypothalamus. To investigate photoperiod-responsive genes, Siberian hamsters were maintained in long or short photoperiods that generate physiological states of obesity or leanness. Microarray expression analysis first identified CRBP1 as a photoperiod-responsive gene, and then further studies using in situ hybridization and immunocytochemistry revealed that expression levels of several related retinoid-signaling genes were modulated in response to photoperiod changes. Genes of the retinoid-signaling pathway, encoding nuclear receptors (RXR/RAR) and retinoid binding proteins (CRBP1 and CRABP2) are photoperiodically regulated in the dorsal tuberomamillary nucleus (DTM): Their expression is significantly lower in short photoperiods and parallels body weight decreases. Studies in pinealectomized hamsters confirm that the pineal melatonin rhythm is necessary for these seasonal changes, and studies in testosterone-treated hamsters reveal that these changes in gene expression are not the secondary consequence of photoperiod-induced changes in steroid levels. Comparative studies using Syrian hamsters, which show divergent seasonal body weight responses to Siberian hamsters when exposed to short photoperiods, showed a distinct pattern of changes in retinoid gene expression in the DTM in response to a change in photoperiod. We infer that the DTM may be an important integrating center for photoperiodic control of seasonal physiology and suggest that the changes in retinoid X receptor gamma expression may be associated with seasonal changes in body weight and energy metabolism.

AB - This study reports novel events related to photoperiodic programming of the neuroendocrine hypothalamus. To investigate photoperiod-responsive genes, Siberian hamsters were maintained in long or short photoperiods that generate physiological states of obesity or leanness. Microarray expression analysis first identified CRBP1 as a photoperiod-responsive gene, and then further studies using in situ hybridization and immunocytochemistry revealed that expression levels of several related retinoid-signaling genes were modulated in response to photoperiod changes. Genes of the retinoid-signaling pathway, encoding nuclear receptors (RXR/RAR) and retinoid binding proteins (CRBP1 and CRABP2) are photoperiodically regulated in the dorsal tuberomamillary nucleus (DTM): Their expression is significantly lower in short photoperiods and parallels body weight decreases. Studies in pinealectomized hamsters confirm that the pineal melatonin rhythm is necessary for these seasonal changes, and studies in testosterone-treated hamsters reveal that these changes in gene expression are not the secondary consequence of photoperiod-induced changes in steroid levels. Comparative studies using Syrian hamsters, which show divergent seasonal body weight responses to Siberian hamsters when exposed to short photoperiods, showed a distinct pattern of changes in retinoid gene expression in the DTM in response to a change in photoperiod. We infer that the DTM may be an important integrating center for photoperiodic control of seasonal physiology and suggest that the changes in retinoid X receptor gamma expression may be associated with seasonal changes in body weight and energy metabolism.

KW - Siberian hamsters

KW - Syrian-hamsters

KW - gene-expression

KW - food-intake

KW - suprachiasmatic nucleus

KW - melatonin infusions

KW - Djungarian hamsters

KW - phodopus-sungorus

KW - pars tuberalis

KW - induced cycles

U2 - 10.1210/en.2003-0838

DO - 10.1210/en.2003-0838

M3 - Article

VL - 145

SP - 13

EP - 20

JO - Endocrinology

JF - Endocrinology

SN - 0013-7227

IS - 1

ER -