Phylogenetic diversity of stress signalling pathways in fungi

Elissavet Nikolaou, Ino Agrafioti, Michael Stumpf, Janet Quinn, Ian Stansfield, Alistair JP Brown

Research output: Contribution to journalArticle

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Abstract

BACKGROUND: Microbes must sense environmental stresses, transduce these signals and mount protective responses to survive in hostile environments. In this study we have tested the hypothesis that fungal stress signalling pathways have evolved rapidly in a niche-specific fashion that is independent of phylogeny. To test this hypothesis we have compared the conservation of stress signalling molecules in diverse fungal species with their stress resistance. These fungi, which include ascomycetes, basidiomycetes and microsporidia, occupy highly divergent niches from saline environments to plant or mammalian hosts. RESULTS: The fungi displayed significant variation in their resistance to osmotic (NaCl and sorbitol), oxidative (H2O2 and menadione) and cell wall stresses (Calcofluor White and Congo Red). There was no strict correlation between fungal phylogeny and stress resistance. Rather, the human pathogens tended to be more resistant to all three types of stress, an exception being the sensitivity of Candida albicans to the cell wall stress, Calcofluor White. In contrast, the plant pathogens were relatively sensitive to oxidative stress. The degree of conservation of osmotic, oxidative and cell wall stress signalling pathways amongst the eighteen fungal species was examined. Putative orthologues of functionally defined signalling components in Saccharomyces cerevisiae were identified by performing reciprocal BLASTP searches, and the percent amino acid identities of these orthologues recorded. This revealed that in general, central components of the osmotic, oxidative and cell wall stress signalling pathways are relatively well conserved, whereas the sensors lying upstream and transcriptional regulators lying downstream of these modules have diverged significantly. There was no obvious correlation between the degree of conservation of stress signalling pathways and the resistance of a particular fungus to the corresponding stress. CONCLUSION: Our data are consistent with the hypothesis that fungal stress signalling components have undergone rapid recent evolution to tune the stress responses in a niche-specific fashion.
Original languageEnglish
Pages (from-to)44
Number of pages1
JournalBMC Evolutionary Biology
Volume9
DOIs
Publication statusPublished - 21 Feb 2009

Fingerprint

cell walls
fungus
phylogenetics
fungi
niches
phylogeny
stress tolerance
menadione
Republic of the Congo
Microsporidia
stress resistance
sorbitol
niche
Basidiomycota
Candida albicans
Ascomycota
plant pathogens
Saccharomyces cerevisiae
stress response
oxidative stress

Keywords

  • Benzenesulfonates
  • Candida albicans
  • Cell Wall
  • Congo Red
  • DNA, Fungal
  • Evolution, Molecular
  • Fungal Proteins
  • Gene Expression Regulation, Fungal
  • Genome, Fungal
  • Hydrogen Peroxide
  • Osmotic Pressure
  • Oxidative Stress
  • Phylogeny
  • Saccharomyces cerevisiae
  • Signal Transduction
  • Sodium Chloride
  • Sorbitol
  • Vitamin K 3

Cite this

Phylogenetic diversity of stress signalling pathways in fungi. / Nikolaou, Elissavet; Agrafioti, Ino; Stumpf, Michael; Quinn, Janet; Stansfield, Ian; Brown, Alistair JP.

In: BMC Evolutionary Biology, Vol. 9, 21.02.2009, p. 44.

Research output: Contribution to journalArticle

Nikolaou, Elissavet ; Agrafioti, Ino ; Stumpf, Michael ; Quinn, Janet ; Stansfield, Ian ; Brown, Alistair JP. / Phylogenetic diversity of stress signalling pathways in fungi. In: BMC Evolutionary Biology. 2009 ; Vol. 9. pp. 44.
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T1 - Phylogenetic diversity of stress signalling pathways in fungi

AU - Nikolaou, Elissavet

AU - Agrafioti, Ino

AU - Stumpf, Michael

AU - Quinn, Janet

AU - Stansfield, Ian

AU - Brown, Alistair JP

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AB - BACKGROUND: Microbes must sense environmental stresses, transduce these signals and mount protective responses to survive in hostile environments. In this study we have tested the hypothesis that fungal stress signalling pathways have evolved rapidly in a niche-specific fashion that is independent of phylogeny. To test this hypothesis we have compared the conservation of stress signalling molecules in diverse fungal species with their stress resistance. These fungi, which include ascomycetes, basidiomycetes and microsporidia, occupy highly divergent niches from saline environments to plant or mammalian hosts. RESULTS: The fungi displayed significant variation in their resistance to osmotic (NaCl and sorbitol), oxidative (H2O2 and menadione) and cell wall stresses (Calcofluor White and Congo Red). There was no strict correlation between fungal phylogeny and stress resistance. Rather, the human pathogens tended to be more resistant to all three types of stress, an exception being the sensitivity of Candida albicans to the cell wall stress, Calcofluor White. In contrast, the plant pathogens were relatively sensitive to oxidative stress. The degree of conservation of osmotic, oxidative and cell wall stress signalling pathways amongst the eighteen fungal species was examined. Putative orthologues of functionally defined signalling components in Saccharomyces cerevisiae were identified by performing reciprocal BLASTP searches, and the percent amino acid identities of these orthologues recorded. This revealed that in general, central components of the osmotic, oxidative and cell wall stress signalling pathways are relatively well conserved, whereas the sensors lying upstream and transcriptional regulators lying downstream of these modules have diverged significantly. There was no obvious correlation between the degree of conservation of stress signalling pathways and the resistance of a particular fungus to the corresponding stress. CONCLUSION: Our data are consistent with the hypothesis that fungal stress signalling components have undergone rapid recent evolution to tune the stress responses in a niche-specific fashion.

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KW - Gene Expression Regulation, Fungal

KW - Genome, Fungal

KW - Hydrogen Peroxide

KW - Osmotic Pressure

KW - Oxidative Stress

KW - Phylogeny

KW - Saccharomyces cerevisiae

KW - Signal Transduction

KW - Sodium Chloride

KW - Sorbitol

KW - Vitamin K 3

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JO - BMC Evolutionary Biology

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