Phylogenetic relationships of butyrate-producing bacteria from the human gut

A Barcenilla, S E Pryde, J C Martin, S H Duncan, C S Stewart, C Henderson, H J Flint

Research output: Contribution to journalArticle

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Abstract

Butyrate is a preferred energy source for colonic epithelial cells and is thought to play an important role in maintaining colonic health in humans. In order to investigate the diversity and stability of butyrate-producing organisms of the colonic flora, anaerobic butyrate-producing bacteria were isolated from freshly voided human fecal samples from three healthy individuals: an infant, an adult omnivore, and an adult vegetarian. A second isolation was performed on the same three individuals 1 year later. Of a total of 313 bacterial isolates, 74 produced more than 2 mM butyrate in vitro. Butyrate-producing isolates were grouped by 16S ribosomal DNA (rDNA) PCR-restriction fragment length polymorphism analysis. The results indicate very little overlap between the predominant ribotypes of the three subjects; furthermore, the flora of each individual changed significantly between the two isolations, Complete sequences of 16S rDNAs were determined fur 24 representative strains and subjected to phylogenetic analysis, Eighty percent of the butyrate-producing isolates fell within the XIVa cluster of gram-positive bacteria as defined by M. D. Collins et at. (Int. J. Syst. Bacteriol. 44:812-826, 1994) and A. Willems et al. (Int. J. Syst. Bacterial. 46:195-199, 1996), with the most abundant group (10 of 24 or 42%) clustering with Eubacterium rectale, Eubacterium ramulus, and Roseburia cecicola, Fifty percent of the butyrate-producing isolates were net acetate consumers during growth, suggesting that they employ the butyryl coenzyme A-acetyl coenzyme A transferase pathway for butyrate production, In contrast, only 1% of the 239 non-butyrate-producing isolates consumed acetate.

Original languageEnglish
Pages (from-to)1654-1661
Number of pages8
JournalApplied and Environmental Microbiology
Volume66
Issue number4
DOIs
Publication statusPublished - Apr 2000

Keywords

  • ribosomal-RNA gene
  • chain fatty-acids
  • anaerobic-bacteria
  • Escherichia-coli
  • human feces
  • sequence
  • butyrivibrio
  • clostridium
  • populations
  • samples

Cite this

Phylogenetic relationships of butyrate-producing bacteria from the human gut. / Barcenilla, A ; Pryde, S E ; Martin, J C ; Duncan, S H ; Stewart, C S ; Henderson, C ; Flint, H J .

In: Applied and Environmental Microbiology, Vol. 66, No. 4, 04.2000, p. 1654-1661.

Research output: Contribution to journalArticle

Barcenilla, A ; Pryde, S E ; Martin, J C ; Duncan, S H ; Stewart, C S ; Henderson, C ; Flint, H J . / Phylogenetic relationships of butyrate-producing bacteria from the human gut. In: Applied and Environmental Microbiology. 2000 ; Vol. 66, No. 4. pp. 1654-1661.
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T1 - Phylogenetic relationships of butyrate-producing bacteria from the human gut

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AU - Pryde, S E

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AU - Stewart, C S

AU - Henderson, C

AU - Flint, H J

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N2 - Butyrate is a preferred energy source for colonic epithelial cells and is thought to play an important role in maintaining colonic health in humans. In order to investigate the diversity and stability of butyrate-producing organisms of the colonic flora, anaerobic butyrate-producing bacteria were isolated from freshly voided human fecal samples from three healthy individuals: an infant, an adult omnivore, and an adult vegetarian. A second isolation was performed on the same three individuals 1 year later. Of a total of 313 bacterial isolates, 74 produced more than 2 mM butyrate in vitro. Butyrate-producing isolates were grouped by 16S ribosomal DNA (rDNA) PCR-restriction fragment length polymorphism analysis. The results indicate very little overlap between the predominant ribotypes of the three subjects; furthermore, the flora of each individual changed significantly between the two isolations, Complete sequences of 16S rDNAs were determined fur 24 representative strains and subjected to phylogenetic analysis, Eighty percent of the butyrate-producing isolates fell within the XIVa cluster of gram-positive bacteria as defined by M. D. Collins et at. (Int. J. Syst. Bacteriol. 44:812-826, 1994) and A. Willems et al. (Int. J. Syst. Bacterial. 46:195-199, 1996), with the most abundant group (10 of 24 or 42%) clustering with Eubacterium rectale, Eubacterium ramulus, and Roseburia cecicola, Fifty percent of the butyrate-producing isolates were net acetate consumers during growth, suggesting that they employ the butyryl coenzyme A-acetyl coenzyme A transferase pathway for butyrate production, In contrast, only 1% of the 239 non-butyrate-producing isolates consumed acetate.

AB - Butyrate is a preferred energy source for colonic epithelial cells and is thought to play an important role in maintaining colonic health in humans. In order to investigate the diversity and stability of butyrate-producing organisms of the colonic flora, anaerobic butyrate-producing bacteria were isolated from freshly voided human fecal samples from three healthy individuals: an infant, an adult omnivore, and an adult vegetarian. A second isolation was performed on the same three individuals 1 year later. Of a total of 313 bacterial isolates, 74 produced more than 2 mM butyrate in vitro. Butyrate-producing isolates were grouped by 16S ribosomal DNA (rDNA) PCR-restriction fragment length polymorphism analysis. The results indicate very little overlap between the predominant ribotypes of the three subjects; furthermore, the flora of each individual changed significantly between the two isolations, Complete sequences of 16S rDNAs were determined fur 24 representative strains and subjected to phylogenetic analysis, Eighty percent of the butyrate-producing isolates fell within the XIVa cluster of gram-positive bacteria as defined by M. D. Collins et at. (Int. J. Syst. Bacteriol. 44:812-826, 1994) and A. Willems et al. (Int. J. Syst. Bacterial. 46:195-199, 1996), with the most abundant group (10 of 24 or 42%) clustering with Eubacterium rectale, Eubacterium ramulus, and Roseburia cecicola, Fifty percent of the butyrate-producing isolates were net acetate consumers during growth, suggesting that they employ the butyryl coenzyme A-acetyl coenzyme A transferase pathway for butyrate production, In contrast, only 1% of the 239 non-butyrate-producing isolates consumed acetate.

KW - ribosomal-RNA gene

KW - chain fatty-acids

KW - anaerobic-bacteria

KW - Escherichia-coli

KW - human feces

KW - sequence

KW - butyrivibrio

KW - clostridium

KW - populations

KW - samples

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M3 - Article

VL - 66

SP - 1654

EP - 1661

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

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ER -