Phylogeny of cytokines: molecular cloning and expression analysis of sea bass Dicentrarchus labrax interleukin-1 beta

Giuseppe Scapigliati, F Buonocore, Steven Bird, Jun Zou, P Pelegrin, C Falasca, D Prugnoli, Christopher John Secombes

Research output: Contribution to journalArticle

112 Citations (Scopus)

Abstract

In this paper the cloning of interleukin-1 beta (IL-1 beta) from the fish Dicentrarchus labrax (sea bass) is described. Using degenerate primers designed from known IL-1 beta sequences, a cDNA fragment was amplified by PCR and elongated by 3' and 5' RACE to give the full-length coding sequence for sea bass IL-1 beta. The cDNA is 1292 bp, lacks a putative ICE cut site, and codes for a deduced peptide of 29.4 kDa with a pI of 5.1. Sequence analysis showed highest amino acid similarity with rainbow trout (62%), Xenopus (46%), and carp (45.5%) IL-1 beta sequences. Expression studies show that sea bass IL-1 beta can be upregulated by bacterial lipopolysaccharide both in vitro and in vivo in leucocytes from blood, head-kidney, spleen, gills and liver, whereas the IL-1 beta transcript was not detectable in thymus and gut-associated lymphoid tissue. Northern blot analysis with head-kidney leucocyte RNA showed a main LPS-upregulated band at 1.3 kb, and two minor bands at 0.9 and 3.0 kb, respectively. Phylogenetic comparisons with IL-1 beta from other vertebrates is presented. (C) 2001 Academic Press.

Original languageEnglish
Pages (from-to)711-726
Number of pages16
JournalFish & Shellfish Immunology
Volume11
Issue number8
DOIs
Publication statusPublished - Nov 2001

Keywords

  • interleukin-1 beta
  • fish
  • sea bass Dicentrarchus labrax
  • cytokines
  • cytokine evolution
  • leucocytes
  • trout oncorhynchus-mykiss
  • sequence
  • cells
  • macrophages
  • alighnment
  • monocytes
  • gene
  • acid

Cite this

Scapigliati, G., Buonocore, F., Bird, S., Zou, J., Pelegrin, P., Falasca, C., ... Secombes, C. J. (2001). Phylogeny of cytokines: molecular cloning and expression analysis of sea bass Dicentrarchus labrax interleukin-1 beta. Fish & Shellfish Immunology, 11(8), 711-726. https://doi.org/10.1006/fsim.2001.0347

Phylogeny of cytokines : molecular cloning and expression analysis of sea bass Dicentrarchus labrax interleukin-1 beta. / Scapigliati, Giuseppe; Buonocore, F ; Bird, Steven; Zou, Jun; Pelegrin, P ; Falasca, C ; Prugnoli, D ; Secombes, Christopher John.

In: Fish & Shellfish Immunology, Vol. 11, No. 8, 11.2001, p. 711-726.

Research output: Contribution to journalArticle

Scapigliati, G, Buonocore, F, Bird, S, Zou, J, Pelegrin, P, Falasca, C, Prugnoli, D & Secombes, CJ 2001, 'Phylogeny of cytokines: molecular cloning and expression analysis of sea bass Dicentrarchus labrax interleukin-1 beta', Fish & Shellfish Immunology, vol. 11, no. 8, pp. 711-726. https://doi.org/10.1006/fsim.2001.0347
Scapigliati, Giuseppe ; Buonocore, F ; Bird, Steven ; Zou, Jun ; Pelegrin, P ; Falasca, C ; Prugnoli, D ; Secombes, Christopher John. / Phylogeny of cytokines : molecular cloning and expression analysis of sea bass Dicentrarchus labrax interleukin-1 beta. In: Fish & Shellfish Immunology. 2001 ; Vol. 11, No. 8. pp. 711-726.
@article{9bfdc32db17b48d6ac063ffd2ce6269a,
title = "Phylogeny of cytokines: molecular cloning and expression analysis of sea bass Dicentrarchus labrax interleukin-1 beta",
abstract = "In this paper the cloning of interleukin-1 beta (IL-1 beta) from the fish Dicentrarchus labrax (sea bass) is described. Using degenerate primers designed from known IL-1 beta sequences, a cDNA fragment was amplified by PCR and elongated by 3' and 5' RACE to give the full-length coding sequence for sea bass IL-1 beta. The cDNA is 1292 bp, lacks a putative ICE cut site, and codes for a deduced peptide of 29.4 kDa with a pI of 5.1. Sequence analysis showed highest amino acid similarity with rainbow trout (62{\%}), Xenopus (46{\%}), and carp (45.5{\%}) IL-1 beta sequences. Expression studies show that sea bass IL-1 beta can be upregulated by bacterial lipopolysaccharide both in vitro and in vivo in leucocytes from blood, head-kidney, spleen, gills and liver, whereas the IL-1 beta transcript was not detectable in thymus and gut-associated lymphoid tissue. Northern blot analysis with head-kidney leucocyte RNA showed a main LPS-upregulated band at 1.3 kb, and two minor bands at 0.9 and 3.0 kb, respectively. Phylogenetic comparisons with IL-1 beta from other vertebrates is presented. (C) 2001 Academic Press.",
keywords = "interleukin-1 beta, fish, sea bass Dicentrarchus labrax, cytokines, cytokine evolution, leucocytes, trout oncorhynchus-mykiss, sequence, cells, macrophages, alighnment, monocytes, gene, acid",
author = "Giuseppe Scapigliati and F Buonocore and Steven Bird and Jun Zou and P Pelegrin and C Falasca and D Prugnoli and Secombes, {Christopher John}",
year = "2001",
month = "11",
doi = "10.1006/fsim.2001.0347",
language = "English",
volume = "11",
pages = "711--726",
journal = "Fish & Shellfish Immunology",
issn = "1050-4648",
publisher = "ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD",
number = "8",

}

TY - JOUR

T1 - Phylogeny of cytokines

T2 - molecular cloning and expression analysis of sea bass Dicentrarchus labrax interleukin-1 beta

AU - Scapigliati, Giuseppe

AU - Buonocore, F

AU - Bird, Steven

AU - Zou, Jun

AU - Pelegrin, P

AU - Falasca, C

AU - Prugnoli, D

AU - Secombes, Christopher John

PY - 2001/11

Y1 - 2001/11

N2 - In this paper the cloning of interleukin-1 beta (IL-1 beta) from the fish Dicentrarchus labrax (sea bass) is described. Using degenerate primers designed from known IL-1 beta sequences, a cDNA fragment was amplified by PCR and elongated by 3' and 5' RACE to give the full-length coding sequence for sea bass IL-1 beta. The cDNA is 1292 bp, lacks a putative ICE cut site, and codes for a deduced peptide of 29.4 kDa with a pI of 5.1. Sequence analysis showed highest amino acid similarity with rainbow trout (62%), Xenopus (46%), and carp (45.5%) IL-1 beta sequences. Expression studies show that sea bass IL-1 beta can be upregulated by bacterial lipopolysaccharide both in vitro and in vivo in leucocytes from blood, head-kidney, spleen, gills and liver, whereas the IL-1 beta transcript was not detectable in thymus and gut-associated lymphoid tissue. Northern blot analysis with head-kidney leucocyte RNA showed a main LPS-upregulated band at 1.3 kb, and two minor bands at 0.9 and 3.0 kb, respectively. Phylogenetic comparisons with IL-1 beta from other vertebrates is presented. (C) 2001 Academic Press.

AB - In this paper the cloning of interleukin-1 beta (IL-1 beta) from the fish Dicentrarchus labrax (sea bass) is described. Using degenerate primers designed from known IL-1 beta sequences, a cDNA fragment was amplified by PCR and elongated by 3' and 5' RACE to give the full-length coding sequence for sea bass IL-1 beta. The cDNA is 1292 bp, lacks a putative ICE cut site, and codes for a deduced peptide of 29.4 kDa with a pI of 5.1. Sequence analysis showed highest amino acid similarity with rainbow trout (62%), Xenopus (46%), and carp (45.5%) IL-1 beta sequences. Expression studies show that sea bass IL-1 beta can be upregulated by bacterial lipopolysaccharide both in vitro and in vivo in leucocytes from blood, head-kidney, spleen, gills and liver, whereas the IL-1 beta transcript was not detectable in thymus and gut-associated lymphoid tissue. Northern blot analysis with head-kidney leucocyte RNA showed a main LPS-upregulated band at 1.3 kb, and two minor bands at 0.9 and 3.0 kb, respectively. Phylogenetic comparisons with IL-1 beta from other vertebrates is presented. (C) 2001 Academic Press.

KW - interleukin-1 beta

KW - fish

KW - sea bass Dicentrarchus labrax

KW - cytokines

KW - cytokine evolution

KW - leucocytes

KW - trout oncorhynchus-mykiss

KW - sequence

KW - cells

KW - macrophages

KW - alighnment

KW - monocytes

KW - gene

KW - acid

U2 - 10.1006/fsim.2001.0347

DO - 10.1006/fsim.2001.0347

M3 - Article

VL - 11

SP - 711

EP - 726

JO - Fish & Shellfish Immunology

JF - Fish & Shellfish Immunology

SN - 1050-4648

IS - 8

ER -