Physicochemical Characterization of Acidiphilium sp. Biofilms

Moustafa Malki, Santiago Casado, Maria Francisca Lopez, Renaud Caillard, F. Javier Palomares, Jose A. Martin Gago, Cristina Vaz-Dominguez, Angel Cuesta, Ricardo Amils, Victor M. Fernandez, Marisela Velez, Antonio L. De Lacey, David Olea

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The biofilm formation of a strain of the extremophile bacterium Acidiphilium sp., capable of donating electrons directly to electrodes, was studied by different surface characterization techniques. We develop a method that allows the simultaneous study of bacterial biofilms by means of fluorescence microscopy and atomic force microscopy (AFM), in which transparent graphitic flakes deposited on a glass substrate are used as a support for the biofilm. The majority of the cells present on the surface were viable, and the growth of the biofilms over time showed a critical increase of the extracellular polymeric substances (EPS) as well as the formation of nanosized particles inside the biofilm. Also, the presence of Fe in Acidiphilium biofilms was determined by X-ray photoelectron spectroscopy (XPS), whereas surface-enhanced infrared absorption spectroscopy indicated the presence of redox-active proteins.
Original languageEnglish
Pages (from-to)1237-1244
Number of pages8
JournalChemPhysChem
Volume14
Issue number6
Early online date1 Mar 2013
DOIs
Publication statusPublished - 15 Apr 2013

Fingerprint

biofilms
Biofilms
Fluorescence microscopy
flakes
Infrared absorption
Absorption spectroscopy
bacteria
infrared absorption
Infrared spectroscopy
Atomic force microscopy
Bacteria
absorption spectroscopy
X ray photoelectron spectroscopy
infrared spectroscopy
photoelectron spectroscopy
atomic force microscopy
microscopy
proteins
Glass
fluorescence

Keywords

  • atomic force microscopy
  • biofilms
  • graphite
  • redox reactions
  • X-ray photoelectron spectroscopy

Cite this

Malki, M., Casado, S., Lopez, M. F., Caillard, R., Palomares, F. J., Martin Gago, J. A., ... Olea, D. (2013). Physicochemical Characterization of Acidiphilium sp. Biofilms. ChemPhysChem, 14(6), 1237-1244. https://doi.org/10.1002/cphc.201201034

Physicochemical Characterization of Acidiphilium sp. Biofilms. / Malki, Moustafa; Casado, Santiago; Lopez, Maria Francisca; Caillard, Renaud; Palomares, F. Javier; Martin Gago, Jose A.; Vaz-Dominguez, Cristina; Cuesta, Angel; Amils, Ricardo; Fernandez, Victor M.; Velez, Marisela; De Lacey, Antonio L.; Olea, David.

In: ChemPhysChem, Vol. 14, No. 6, 15.04.2013, p. 1237-1244.

Research output: Contribution to journalArticle

Malki, M, Casado, S, Lopez, MF, Caillard, R, Palomares, FJ, Martin Gago, JA, Vaz-Dominguez, C, Cuesta, A, Amils, R, Fernandez, VM, Velez, M, De Lacey, AL & Olea, D 2013, 'Physicochemical Characterization of Acidiphilium sp. Biofilms', ChemPhysChem, vol. 14, no. 6, pp. 1237-1244. https://doi.org/10.1002/cphc.201201034
Malki M, Casado S, Lopez MF, Caillard R, Palomares FJ, Martin Gago JA et al. Physicochemical Characterization of Acidiphilium sp. Biofilms. ChemPhysChem. 2013 Apr 15;14(6):1237-1244. https://doi.org/10.1002/cphc.201201034
Malki, Moustafa ; Casado, Santiago ; Lopez, Maria Francisca ; Caillard, Renaud ; Palomares, F. Javier ; Martin Gago, Jose A. ; Vaz-Dominguez, Cristina ; Cuesta, Angel ; Amils, Ricardo ; Fernandez, Victor M. ; Velez, Marisela ; De Lacey, Antonio L. ; Olea, David. / Physicochemical Characterization of Acidiphilium sp. Biofilms. In: ChemPhysChem. 2013 ; Vol. 14, No. 6. pp. 1237-1244.
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