TY - JOUR
T1 - Physicochemical Characterization of Acidiphilium sp. Biofilms
AU - Malki, Moustafa
AU - Casado, Santiago
AU - Lopez, Maria Francisca
AU - Caillard, Renaud
AU - Palomares, F. Javier
AU - Martin Gago, Jose A.
AU - Vaz-Dominguez, Cristina
AU - Cuesta, Angel
AU - Amils, Ricardo
AU - Fernandez, Victor M.
AU - Velez, Marisela
AU - De Lacey, Antonio L.
AU - Olea, David
PY - 2013/4/15
Y1 - 2013/4/15
N2 - The biofilm formation of a strain of the extremophile bacterium Acidiphilium sp., capable of donating electrons directly to electrodes, was studied by different surface characterization techniques. We develop a method that allows the simultaneous study of bacterial biofilms by means of fluorescence microscopy and atomic force microscopy (AFM), in which transparent graphitic flakes deposited on a glass substrate are used as a support for the biofilm. The majority of the cells present on the surface were viable, and the growth of the biofilms over time showed a critical increase of the extracellular polymeric substances (EPS) as well as the formation of nanosized particles inside the biofilm. Also, the presence of Fe in Acidiphilium biofilms was determined by X-ray photoelectron spectroscopy (XPS), whereas surface-enhanced infrared absorption spectroscopy indicated the presence of redox-active proteins.
AB - The biofilm formation of a strain of the extremophile bacterium Acidiphilium sp., capable of donating electrons directly to electrodes, was studied by different surface characterization techniques. We develop a method that allows the simultaneous study of bacterial biofilms by means of fluorescence microscopy and atomic force microscopy (AFM), in which transparent graphitic flakes deposited on a glass substrate are used as a support for the biofilm. The majority of the cells present on the surface were viable, and the growth of the biofilms over time showed a critical increase of the extracellular polymeric substances (EPS) as well as the formation of nanosized particles inside the biofilm. Also, the presence of Fe in Acidiphilium biofilms was determined by X-ray photoelectron spectroscopy (XPS), whereas surface-enhanced infrared absorption spectroscopy indicated the presence of redox-active proteins.
KW - atomic force microscopy
KW - biofilms
KW - graphite
KW - redox reactions
KW - X-ray photoelectron spectroscopy
U2 - 10.1002/cphc.201201034
DO - 10.1002/cphc.201201034
M3 - Article
SN - 1439-4235
VL - 14
SP - 1237
EP - 1244
JO - ChemPhysChem
JF - ChemPhysChem
IS - 6
ER -