Physiological analysis of bacterial mechanosensitive channels

Ian R. Booth; Michelle D. Edwards; Susan Shirley Black; Ulrike Schumann; Wendy Bartlett; Tim Rasmussen; Akiko Rasmussen; Samantha Miller

doi:10.1016/S0076-6879(07)28003-6

Physiological analysis of bacterial mechanosensitive channels

Ian R. Booth, Michelle D. Edwards, Susan Shirley Black, Ulrike Schumann, Wendy Bartlett, Tim Rasmussen, Akiko Rasmussen, Samantha Miller

Medical Sciences

Research output: Chapter in Book/Report/Conference proceeding › Chapter

21 Citations (Scopus)

Abstract

Bacterial mechanosensitive (MS) channels play a significant role in protecting cells against hypoosmotic shock. Bacteria that have been diluted from high osmolarity medium into dilute solution are required to cope with sudden water influx associated with an osmotic imbalance equivalent to 10 to 14 atm. The cell wall is only poorly expansive and the cytoplasmic membrane even less so. Thus, swelling is not an option and the cell must rapidly eject solutes to diminish the osmotic gradient and thereby preserve structural integrity. This chapter describes cellular assays of MS channel function and their interpretation.

Original language	English
Title of host publication	Methods in Enzymology
Subtitle of host publication	Osmosensing and Osmosignaling
Editors	Dieter Häussinger, Helmut Sies
Publisher	Academic Press
Pages	47-61
Number of pages	15
Volume	428
ISBN (Print)	978-0-12-373921-6
DOIs	https://doi.org/10.1016/S0076-6879(07)28003-6
Publication status	Published - 2007

Publication series

Name	Methods in Enzymology

Keywords

escherichia-coli MSCS
large-conductance
compatible solutes
osmotic-stress
activation
protein
osmoregulation
identification
mutations
stability

Access to Document

10.1016/S0076-6879(07)28003-6

Cite this

Booth, I. R., Edwards, M. D., Black, S. S., Schumann, U., Bartlett, W., Rasmussen, T., Rasmussen, A., & Miller, S. (2007). Physiological analysis of bacterial mechanosensitive channels. In D. Häussinger, & H. Sies (Eds.), Methods in Enzymology: Osmosensing and Osmosignaling (Vol. 428, pp. 47-61). (Methods in Enzymology). Academic Press. https://doi.org/10.1016/S0076-6879(07)28003-6

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abstract = "Bacterial mechanosensitive (MS) channels play a significant role in protecting cells against hypoosmotic shock. Bacteria that have been diluted from high osmolarity medium into dilute solution are required to cope with sudden water influx associated with an osmotic imbalance equivalent to 10 to 14 atm. The cell wall is only poorly expansive and the cytoplasmic membrane even less so. Thus, swelling is not an option and the cell must rapidly eject solutes to diminish the osmotic gradient and thereby preserve structural integrity. This chapter describes cellular assays of MS channel function and their interpretation.",

keywords = "escherichia-coli MSCS, large-conductance, compatible solutes, osmotic-stress, activation, protein, osmoregulation, identification, mutations, stability",

author = "Booth, {Ian R.} and Edwards, {Michelle D.} and Black, {Susan Shirley} and Ulrike Schumann and Wendy Bartlett and Tim Rasmussen and Akiko Rasmussen and Samantha Miller",

year = "2007",

doi = "10.1016/S0076-6879(07)28003-6",

language = "English",

isbn = "978-0-12-373921-6",

volume = "428",

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pages = "47--61",

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T1 - Physiological analysis of bacterial mechanosensitive channels

AU - Booth, Ian R.

AU - Edwards, Michelle D.

AU - Black, Susan Shirley

AU - Schumann, Ulrike

AU - Bartlett, Wendy

AU - Rasmussen, Tim

AU - Rasmussen, Akiko

AU - Miller, Samantha

PY - 2007

Y1 - 2007

N2 - Bacterial mechanosensitive (MS) channels play a significant role in protecting cells against hypoosmotic shock. Bacteria that have been diluted from high osmolarity medium into dilute solution are required to cope with sudden water influx associated with an osmotic imbalance equivalent to 10 to 14 atm. The cell wall is only poorly expansive and the cytoplasmic membrane even less so. Thus, swelling is not an option and the cell must rapidly eject solutes to diminish the osmotic gradient and thereby preserve structural integrity. This chapter describes cellular assays of MS channel function and their interpretation.

AB - Bacterial mechanosensitive (MS) channels play a significant role in protecting cells against hypoosmotic shock. Bacteria that have been diluted from high osmolarity medium into dilute solution are required to cope with sudden water influx associated with an osmotic imbalance equivalent to 10 to 14 atm. The cell wall is only poorly expansive and the cytoplasmic membrane even less so. Thus, swelling is not an option and the cell must rapidly eject solutes to diminish the osmotic gradient and thereby preserve structural integrity. This chapter describes cellular assays of MS channel function and their interpretation.

KW - escherichia-coli MSCS

KW - large-conductance

KW - compatible solutes

KW - osmotic-stress

KW - activation

KW - protein

KW - osmoregulation

KW - identification

KW - mutations

KW - stability

U2 - 10.1016/S0076-6879(07)28003-6

DO - 10.1016/S0076-6879(07)28003-6

M3 - Chapter

SN - 978-0-12-373921-6

VL - 428

T3 - Methods in Enzymology

SP - 47

EP - 61

BT - Methods in Enzymology

A2 - Häussinger, Dieter

A2 - Sies, Helmut

PB - Academic Press

ER -

Physiological analysis of bacterial mechanosensitive channels

Abstract

Publication series

Keywords

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