Phytophthora cactorum (Lebert & Cohn) J. Schröt as causal agent of dieback of chestnut and apple trees in Macedonia

Mihajlo Risteski; Stephen Woodward; Marin Ježić; Rade Rusevski; Biljana Kuzmanovska; Kiril Sotirovski

doi:10.20903/csnmbs.masa.2019.40.1.133

Phytophthora cactorum (Lebert & Cohn) J. Schröt as causal agent of dieback of chestnut and apple trees in Macedonia

Mihajlo Risteski (Corresponding Author), Stephen Woodward, Marin Ježić, Rade Rusevski, Biljana Kuzmanovska, Kiril Sotirovski

Aberdeen Centre For Environmental Sustainability

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Abstract

From 2013–2017, 11 chestnut populations and 16 apple orchards/plantations in Macedonia were examined for health; soil, root and bark samples were collected from trees expressing symptoms regarded as Phytophthora specific. Using leaf baits of Prunus laurocerasus and selective V8 Agar (PARPNH), 19 pure Phytophthora sp. cultures were isolated and identified as P. cactorum by ITS sequencing. Sixteen isolates were from apple trees and 3 from chestnut trees. Phylogenetic analyses suggested slight distance between P. cactorum isolates originating from chestnut trees compared to those from apple orchards. Assessment of pathogenicity using chestnuts twigs showed no differences between P. cactorum isolates from the two tree host species.

Original language	English
Pages (from-to)	93-103
Number of pages	11
Journal	Macedonian Academy of Sciences and Arts, Section: Natural, Mathematical and Biotechnical Sciences
Volume	40
Issue number	1
DOIs	https://doi.org/10.20903/csnmbs.masa.2019.40.1.133
Publication status	Published - 2019

Bibliographical note

Acknowledgments. SCOPES Joint Research Project "Invasive chestnut diseases in the Balkans and Georgia - epidemiological research and management options".
Multilateral project (Switzerland, Croatia, Macedonia and Georgia), 2013-2016 (IZ73Z0_152525).
SEE-ERA.NET PLUS Joint Research Project "Diversity and invading Phytophthora spp. plant pathogens in agro and forest ecosystems in Southeast Europe". Multilateral project (Bulgaria, Macedonia, Greece, Serbia and Romania), 2010-2012.
COST (Cost Action FP0801: Established and Emerging Phytophthora: Increasing Threats to Woodland and Forest Ecosystems.)

Keywords

Malus spp.
Castanea sativa
pathogenicity
phylogenetic analysis

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Phytophthora cactorum (Lebert & Cohn) J. Schröt as causal agent of dieback of chestnut and apple trees in Macedonia
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Risteski, M., Woodward, S., Ježić, M., Rusevski, R., Kuzmanovska, B., & Sotirovski, K. (2019). Phytophthora cactorum (Lebert & Cohn) J. Schröt as causal agent of dieback of chestnut and apple trees in Macedonia. Macedonian Academy of Sciences and Arts, Section: Natural, Mathematical and Biotechnical Sciences, 40(1), 93-103. https://doi.org/10.20903/csnmbs.masa.2019.40.1.133

Phytophthora cactorum (Lebert & Cohn) J. Schröt as causal agent of dieback of chestnut and apple trees in Macedonia. / Risteski, Mihajlo (Corresponding Author); Woodward, Stephen; Ježić, Marin et al.
In: Macedonian Academy of Sciences and Arts, Section: Natural, Mathematical and Biotechnical Sciences, Vol. 40, No. 1, 2019, p. 93-103.

Research output: Contribution to journal › Article › peer-review

Risteski, M, Woodward, S, Ježić, M, Rusevski, R, Kuzmanovska, B & Sotirovski, K 2019, 'Phytophthora cactorum (Lebert & Cohn) J. Schröt as causal agent of dieback of chestnut and apple trees in Macedonia', Macedonian Academy of Sciences and Arts, Section: Natural, Mathematical and Biotechnical Sciences, vol. 40, no. 1, pp. 93-103. https://doi.org/10.20903/csnmbs.masa.2019.40.1.133

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abstract = "From 2013–2017, 11 chestnut populations and 16 apple orchards/plantations in Macedonia were examined for health; soil, root and bark samples were collected from trees expressing symptoms regarded as Phytophthora specific. Using leaf baits of Prunus laurocerasus and selective V8 Agar (PARPNH), 19 pure Phytophthora sp. cultures were isolated and identified as P. cactorum by ITS sequencing. Sixteen isolates were from apple trees and 3 from chestnut trees. Phylogenetic analyses suggested slight distance between P. cactorum isolates originating from chestnut trees compared to those from apple orchards. Assessment of pathogenicity using chestnuts twigs showed no differences between P. cactorum isolates from the two tree host species.",

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Phytophthora cactorum (Lebert & Cohn) J. Schröt as causal agent of dieback of chestnut and apple trees in Macedonia

Abstract

Bibliographical note

Keywords

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