Plasma-membrane fatty-acid-binding protein (FABP(PM)) is exclusively located in the maternal facing membranes of the human placenta

    Research output: Contribution to journalArticle

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    Abstract

    We reported earlier the presence of a 40 kDa plasma membrane fatty acid-binding protein (FABP(pm)) in human placenta, This protein is thought to be involved in the sequestration of unesterified free fatty acids bound to albumin from the maternal plasma for delivery to the fetus, However its location in human placental syncytiotrophoblasts is not known, These cells are bipolar; one side facing maternal circulation (microvillous membranes), and the other side facing fetal circulation (basal membranes), Therefore, it is important to resolve the location of this protein in trophoblast membranes in order to understand fatty acid transport and metabolism in human placenta, Isolated plasma membranes vesicles were prepared respectively from the maternal facing microvillous and fetal facing surface of the human full-term placental syncytiotrophoblast. Using these membrane preparations, fatty acid binding activity, the polyacrylamide gel electrophoresis radiobinding assay for FABP(pm), and Western blot analysis of FABP(pm) were carried out to determine the location of this protein in these membranes. Based on the above studies me conclude that the FABP(pm) is located exclusively in the microvillous membranes, Since FABP(pm) may be responsible for FFA uptake, its location in the microvillous membranes favours the unidirectional flow of maternal FFA to the fetus.

    Original languageEnglish
    Pages (from-to)227-230
    Number of pages4
    JournalFEBS Letters
    Volume375
    Issue number3
    DOIs
    Publication statusPublished - 20 Nov 1995

    Keywords

    • PLASMA MEMBRANE FATTY ACID-BINDING PROTEIN (FABP(PM))
    • SYNCYTIOTROPHOBLAST
    • MICROVILLOUS MEMBRANE
    • BASAL MEMBRANE
    • FATTY ACID UPTAKE
    • HUMAN PLACENTA
    • plasma membrane fatty acid-binding protein (FABP(PM))
    • syncytiotrophoblast
    • microvillous membrane
    • basal membrane
    • fatty acid uptake
    • human placenta

    Cite this

    Plasma-membrane fatty-acid-binding protein (FABP(PM)) is exclusively located in the maternal facing membranes of the human placenta. / Campbell, Fiona Margaret; Duttaroy, A K .

    In: FEBS Letters, Vol. 375, No. 3, 20.11.1995, p. 227-230.

    Research output: Contribution to journalArticle

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    abstract = "We reported earlier the presence of a 40 kDa plasma membrane fatty acid-binding protein (FABP(pm)) in human placenta, This protein is thought to be involved in the sequestration of unesterified free fatty acids bound to albumin from the maternal plasma for delivery to the fetus, However its location in human placental syncytiotrophoblasts is not known, These cells are bipolar; one side facing maternal circulation (microvillous membranes), and the other side facing fetal circulation (basal membranes), Therefore, it is important to resolve the location of this protein in trophoblast membranes in order to understand fatty acid transport and metabolism in human placenta, Isolated plasma membranes vesicles were prepared respectively from the maternal facing microvillous and fetal facing surface of the human full-term placental syncytiotrophoblast. Using these membrane preparations, fatty acid binding activity, the polyacrylamide gel electrophoresis radiobinding assay for FABP(pm), and Western blot analysis of FABP(pm) were carried out to determine the location of this protein in these membranes. Based on the above studies me conclude that the FABP(pm) is located exclusively in the microvillous membranes, Since FABP(pm) may be responsible for FFA uptake, its location in the microvillous membranes favours the unidirectional flow of maternal FFA to the fetus.",
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    N2 - We reported earlier the presence of a 40 kDa plasma membrane fatty acid-binding protein (FABP(pm)) in human placenta, This protein is thought to be involved in the sequestration of unesterified free fatty acids bound to albumin from the maternal plasma for delivery to the fetus, However its location in human placental syncytiotrophoblasts is not known, These cells are bipolar; one side facing maternal circulation (microvillous membranes), and the other side facing fetal circulation (basal membranes), Therefore, it is important to resolve the location of this protein in trophoblast membranes in order to understand fatty acid transport and metabolism in human placenta, Isolated plasma membranes vesicles were prepared respectively from the maternal facing microvillous and fetal facing surface of the human full-term placental syncytiotrophoblast. Using these membrane preparations, fatty acid binding activity, the polyacrylamide gel electrophoresis radiobinding assay for FABP(pm), and Western blot analysis of FABP(pm) were carried out to determine the location of this protein in these membranes. Based on the above studies me conclude that the FABP(pm) is located exclusively in the microvillous membranes, Since FABP(pm) may be responsible for FFA uptake, its location in the microvillous membranes favours the unidirectional flow of maternal FFA to the fetus.

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