TY - JOUR
T1 - Plasma zinc's alter ego is a low-molecular-weight humoral factor
AU - Ou, Ou
AU - Allen-Redpath, Keith
AU - Urgast, Dagmar
AU - Gordon, Margaret-Jane
AU - Campbell, Gill
AU - Feldmann, Jörg
AU - Nixon, Graeme F.
AU - Mayer, Claus-Dieter
AU - Kwun, In-Sook
AU - Beattie, John H.
PY - 2013/9
Y1 - 2013/9
N2 - Mild dietary zinc deprivation in humans and rodents has little effect on blood plasma zinc levels, and yet cellular consequences of zinc depletion can be detected in vascular and other tissues. We proposed that a zinc-regulated humoral factor might mediate the effects of zinc deprivation. Using a novel approach, primary rat vascular smooth muscle cells (VSMCs) were treated with plasma from zinc-deficient (2500 genes, compared to incubation of cells with zinc-adequate rat plasma. We demonstrated that this effect was caused by a low-molecular-weight (~2-kDa) zinc-regulated humoral factor but that changes in gene expression were mostly reversed by adding zinc back to zinc-deficient plasma. Strongly regulated genes were overrepresented in pathways associated with immune function and development. We conclude that zinc deficiency induces the production of a low-molecular-weight humoral factor whose influence on VSMC gene expression is blocked by plasma zinc. This factor is therefore under dual control by zinc.- Ou, O., Allen-Redpath, K., Urgast, D., Gordon, M.-J., Campbell, G., Feldmann, J., Nixon, G. F., Mayer, C.-D., Kwun, I.-S., and Beattie, J. H. Plasma zinc's alter ego is a low-molecular-weight humoral factor.
AB - Mild dietary zinc deprivation in humans and rodents has little effect on blood plasma zinc levels, and yet cellular consequences of zinc depletion can be detected in vascular and other tissues. We proposed that a zinc-regulated humoral factor might mediate the effects of zinc deprivation. Using a novel approach, primary rat vascular smooth muscle cells (VSMCs) were treated with plasma from zinc-deficient (2500 genes, compared to incubation of cells with zinc-adequate rat plasma. We demonstrated that this effect was caused by a low-molecular-weight (~2-kDa) zinc-regulated humoral factor but that changes in gene expression were mostly reversed by adding zinc back to zinc-deficient plasma. Strongly regulated genes were overrepresented in pathways associated with immune function and development. We conclude that zinc deficiency induces the production of a low-molecular-weight humoral factor whose influence on VSMC gene expression is blocked by plasma zinc. This factor is therefore under dual control by zinc.- Ou, O., Allen-Redpath, K., Urgast, D., Gordon, M.-J., Campbell, G., Feldmann, J., Nixon, G. F., Mayer, C.-D., Kwun, I.-S., and Beattie, J. H. Plasma zinc's alter ego is a low-molecular-weight humoral factor.
KW - atherosclerosis
KW - microarray
KW - smooth muscle
KW - zinc deficiency
UR - http://www.scopus.com/inward/record.url?scp=84881278151&partnerID=8YFLogxK
U2 - 10.1096/fj.13-228791
DO - 10.1096/fj.13-228791
M3 - Article
AN - SCOPUS:84881278151
SN - 0892-6638
VL - 27
SP - 3672
EP - 3682
JO - The FASEB Journal
JF - The FASEB Journal
IS - 9
ER -