Platelet-derived microparticle count and surface molecule expression differ between subjects with and without type 2 diabetes, independently of obesity status

Xuguang Zhang, Susan C McGeoch, Alexandra M Johnstone, Grietje Holtrop, Alan A Sneddon, Sandra M Macrury, Ian L Megson, Donald W M Pearson, Prakash Abraham, Baukje De Roos, Gerald E Lobley, Niamh O'Kennedy

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Abstract

This study investigated the impact of either type 2 diabetes or obesity, separately or in combination, on the absolute amounts of microparticles (MP) and the pathways by which these are associated with either condition. The concentrations of circulating MP derived from platelets (PMP), leukocytes (LMP) and monocytes (MMP), together with their specific activation markers, were compared in 30 subjects who were characterised across 4 cohorts as obese or type 2 diabetes. The subjects with type 2 diabetes had elevated concentrations of total PMP (P = 0.003), and PMP that were fibrinogen-positive (P = 0.04), tissue factor-positive (P < 0.001), P-selectin-positive (P = 0.03). Type 2 diabetes did not alter either total or activated LMP or MMP. Obesity per se did not impact on any MP measurement. Elevated concentrations of plasma PMP occurred in subjects with type 2 diabetes, whether they were obese or non-obese. In contrast, obesity in the absence of type 2 diabetes had no effect. The increased concentrations of specific marker-positive PMP in the subjects with diabetes might reflect potential pathways by which PMP may contribute to the pathogenesis of atherosclerosis and type 2 diabetes.
Original languageEnglish
Pages (from-to)455-463
Number of pages9
JournalJournal of Thrombosis and Thrombolysis
Volume37
Issue number4
Early online date5 Oct 2013
DOIs
Publication statusPublished - May 2014

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Type 2 Diabetes Mellitus
Blood Platelets
Obesity
Matrix Metalloproteinases
P-Selectin
Thromboplastin
Fibrinogen
Monocytes
Atherosclerosis
Leukocytes

Keywords

  • circulating cell-derived microparticles
  • obesity
  • Type 2 diabetes mellitus
  • blood platelets
  • cardiovascular diseases

Cite this

Platelet-derived microparticle count and surface molecule expression differ between subjects with and without type 2 diabetes, independently of obesity status. / Zhang, Xuguang; McGeoch, Susan C; Johnstone, Alexandra M; Holtrop, Grietje; Sneddon, Alan A; Macrury, Sandra M; Megson, Ian L; Pearson, Donald W M; Abraham, Prakash; De Roos, Baukje; Lobley, Gerald E; O'Kennedy, Niamh.

In: Journal of Thrombosis and Thrombolysis, Vol. 37, No. 4, 05.2014, p. 455-463.

Research output: Contribution to journalArticle

Zhang, Xuguang ; McGeoch, Susan C ; Johnstone, Alexandra M ; Holtrop, Grietje ; Sneddon, Alan A ; Macrury, Sandra M ; Megson, Ian L ; Pearson, Donald W M ; Abraham, Prakash ; De Roos, Baukje ; Lobley, Gerald E ; O'Kennedy, Niamh. / Platelet-derived microparticle count and surface molecule expression differ between subjects with and without type 2 diabetes, independently of obesity status. In: Journal of Thrombosis and Thrombolysis. 2014 ; Vol. 37, No. 4. pp. 455-463.
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abstract = "This study investigated the impact of either type 2 diabetes or obesity, separately or in combination, on the absolute amounts of microparticles (MP) and the pathways by which these are associated with either condition. The concentrations of circulating MP derived from platelets (PMP), leukocytes (LMP) and monocytes (MMP), together with their specific activation markers, were compared in 30 subjects who were characterised across 4 cohorts as obese or type 2 diabetes. The subjects with type 2 diabetes had elevated concentrations of total PMP (P = 0.003), and PMP that were fibrinogen-positive (P = 0.04), tissue factor-positive (P < 0.001), P-selectin-positive (P = 0.03). Type 2 diabetes did not alter either total or activated LMP or MMP. Obesity per se did not impact on any MP measurement. Elevated concentrations of plasma PMP occurred in subjects with type 2 diabetes, whether they were obese or non-obese. In contrast, obesity in the absence of type 2 diabetes had no effect. The increased concentrations of specific marker-positive PMP in the subjects with diabetes might reflect potential pathways by which PMP may contribute to the pathogenesis of atherosclerosis and type 2 diabetes.",
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author = "Xuguang Zhang and McGeoch, {Susan C} and Johnstone, {Alexandra M} and Grietje Holtrop and Sneddon, {Alan A} and Macrury, {Sandra M} and Megson, {Ian L} and Pearson, {Donald W M} and Prakash Abraham and {De Roos}, Baukje and Lobley, {Gerald E} and Niamh O'Kennedy",
note = "Acknowledgments: This study was partly funded by a Grant awarded by the Chief Scientists Office of the Scottish Government to the University of the Highlands and Islands, Rowett Institute of Nutrition & Health at the University of Aberdeen and Aberdeen Royal Infirmary to examine the impact of diets rich in oats for life-style control of type 2 diabetes. Additional support was contributed by the Rural & Environment Science & Analytical Services (RESAS) of the Scottish Government to Rowett Institute of Nutrition & Health (RINH). Costs of microparticle anayses were borne by Provexis Plc.",
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AU - McGeoch, Susan C

AU - Johnstone, Alexandra M

AU - Holtrop, Grietje

AU - Sneddon, Alan A

AU - Macrury, Sandra M

AU - Megson, Ian L

AU - Pearson, Donald W M

AU - Abraham, Prakash

AU - De Roos, Baukje

AU - Lobley, Gerald E

AU - O'Kennedy, Niamh

N1 - Acknowledgments: This study was partly funded by a Grant awarded by the Chief Scientists Office of the Scottish Government to the University of the Highlands and Islands, Rowett Institute of Nutrition & Health at the University of Aberdeen and Aberdeen Royal Infirmary to examine the impact of diets rich in oats for life-style control of type 2 diabetes. Additional support was contributed by the Rural & Environment Science & Analytical Services (RESAS) of the Scottish Government to Rowett Institute of Nutrition & Health (RINH). Costs of microparticle anayses were borne by Provexis Plc.

PY - 2014/5

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N2 - This study investigated the impact of either type 2 diabetes or obesity, separately or in combination, on the absolute amounts of microparticles (MP) and the pathways by which these are associated with either condition. The concentrations of circulating MP derived from platelets (PMP), leukocytes (LMP) and monocytes (MMP), together with their specific activation markers, were compared in 30 subjects who were characterised across 4 cohorts as obese or type 2 diabetes. The subjects with type 2 diabetes had elevated concentrations of total PMP (P = 0.003), and PMP that were fibrinogen-positive (P = 0.04), tissue factor-positive (P < 0.001), P-selectin-positive (P = 0.03). Type 2 diabetes did not alter either total or activated LMP or MMP. Obesity per se did not impact on any MP measurement. Elevated concentrations of plasma PMP occurred in subjects with type 2 diabetes, whether they were obese or non-obese. In contrast, obesity in the absence of type 2 diabetes had no effect. The increased concentrations of specific marker-positive PMP in the subjects with diabetes might reflect potential pathways by which PMP may contribute to the pathogenesis of atherosclerosis and type 2 diabetes.

AB - This study investigated the impact of either type 2 diabetes or obesity, separately or in combination, on the absolute amounts of microparticles (MP) and the pathways by which these are associated with either condition. The concentrations of circulating MP derived from platelets (PMP), leukocytes (LMP) and monocytes (MMP), together with their specific activation markers, were compared in 30 subjects who were characterised across 4 cohorts as obese or type 2 diabetes. The subjects with type 2 diabetes had elevated concentrations of total PMP (P = 0.003), and PMP that were fibrinogen-positive (P = 0.04), tissue factor-positive (P < 0.001), P-selectin-positive (P = 0.03). Type 2 diabetes did not alter either total or activated LMP or MMP. Obesity per se did not impact on any MP measurement. Elevated concentrations of plasma PMP occurred in subjects with type 2 diabetes, whether they were obese or non-obese. In contrast, obesity in the absence of type 2 diabetes had no effect. The increased concentrations of specific marker-positive PMP in the subjects with diabetes might reflect potential pathways by which PMP may contribute to the pathogenesis of atherosclerosis and type 2 diabetes.

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KW - Type 2 diabetes mellitus

KW - blood platelets

KW - cardiovascular diseases

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DO - 10.1007/s11239-013-1000-2

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JO - Journal of Thrombosis and Thrombolysis

JF - Journal of Thrombosis and Thrombolysis

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