Polychlorinated biphenyl 126 affects expression of genes involved in stress-immune interaction in primary cultures of rainbow trout anterior kidney cells

E S Quabius, G Krupp, C J Secombes

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19 Citations (Scopus)

Abstract

Stress and immune function are linked in all vertebrates, including teleost fish. Polychlorinated biphenyls (PCBs) are immunotoxic and impair the ability of fish to respond to additional stressors. In this study, we investigated the effects of PCB126 oil stress and immune function and the interaction of these systems in fish using primary Cultures of rainbow trout anterior kidney cells as a model. Gene expression levels of cytochrome P4501A (CYP1A), interleukin-1 beta (1L-1 beta), and glucocorticoid receptor (GR) were measured by real-time quantitative polymerase chain reaction. These genes play important roles in detoxification and immune and stress homeostasis, respectively. Incubation with PCB126 led to increased IL-I beta expression between 30 min and 2 h of exposure, with expression back to basal levels after 6 h. Lipopolysaccharide (LPS) incubation evoked normal IL-1 beta responses after 2 and 24 h PCB incubation. Gene expression levels of GR and CYP1A increased in a time- and dose-dependent manner, reaching a plateau after 12 h of incubation. Preincubation with cortisol resulted in decreased IL-1 beta expression, increased expression of CYP1A and GR, and was accompanied by an abolished PCB responsiveness after more than 4 h of cortisol incubation. We Conclude that PCB 126 exposure is not "stressful," as increased cortisol levels Would result in depressed IL-1 beta expression. Incubation with PCB126 evokes a transient stimulation rather than permanent damage of the immune system, as LPS stimulation resulted in increased IL-1 beta expression after PCB incubation. Prolonged cortisol preincubation, resembling a chronic stress paradigm, negatively affects the immune responsiveness of the cells as well as their capacity for toxicant metabolization.

Original languageEnglish
Pages (from-to)3053-3060
Number of pages8
JournalEnvironmental Toxicology and Chemistry
Volume24
Publication statusPublished - 2005

Keywords

  • trout anterior kidney
  • primary cell culture
  • stress-immune interaction
  • polychlorinated biphenyl 126
  • teleost fish
  • IN-VIVO
  • GLUCOCORTICOID-RECEPTOR
  • ONCORHYNCHUS-MYKISS
  • CONTAMINATED SITES
  • CYPRINUS-CARPIO
  • FISH
  • CORTISOL
  • VITRO
  • INDUCTION
  • LIPOPOLYSACCHARIDE

Cite this

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title = "Polychlorinated biphenyl 126 affects expression of genes involved in stress-immune interaction in primary cultures of rainbow trout anterior kidney cells",
abstract = "Stress and immune function are linked in all vertebrates, including teleost fish. Polychlorinated biphenyls (PCBs) are immunotoxic and impair the ability of fish to respond to additional stressors. In this study, we investigated the effects of PCB126 oil stress and immune function and the interaction of these systems in fish using primary Cultures of rainbow trout anterior kidney cells as a model. Gene expression levels of cytochrome P4501A (CYP1A), interleukin-1 beta (1L-1 beta), and glucocorticoid receptor (GR) were measured by real-time quantitative polymerase chain reaction. These genes play important roles in detoxification and immune and stress homeostasis, respectively. Incubation with PCB126 led to increased IL-I beta expression between 30 min and 2 h of exposure, with expression back to basal levels after 6 h. Lipopolysaccharide (LPS) incubation evoked normal IL-1 beta responses after 2 and 24 h PCB incubation. Gene expression levels of GR and CYP1A increased in a time- and dose-dependent manner, reaching a plateau after 12 h of incubation. Preincubation with cortisol resulted in decreased IL-1 beta expression, increased expression of CYP1A and GR, and was accompanied by an abolished PCB responsiveness after more than 4 h of cortisol incubation. We Conclude that PCB 126 exposure is not {"}stressful,{"} as increased cortisol levels Would result in depressed IL-1 beta expression. Incubation with PCB126 evokes a transient stimulation rather than permanent damage of the immune system, as LPS stimulation resulted in increased IL-1 beta expression after PCB incubation. Prolonged cortisol preincubation, resembling a chronic stress paradigm, negatively affects the immune responsiveness of the cells as well as their capacity for toxicant metabolization.",
keywords = "trout anterior kidney, primary cell culture, stress-immune interaction, polychlorinated biphenyl 126, teleost fish, IN-VIVO, GLUCOCORTICOID-RECEPTOR, ONCORHYNCHUS-MYKISS, CONTAMINATED SITES, CYPRINUS-CARPIO, FISH, CORTISOL, VITRO, INDUCTION, LIPOPOLYSACCHARIDE",
author = "Quabius, {E S} and G Krupp and Secombes, {C J}",
year = "2005",
language = "English",
volume = "24",
pages = "3053--3060",
journal = "Environmental Toxicology and Chemistry",
issn = "0730-7268",
publisher = "John Wiley and Sons Ltd",

}

TY - JOUR

T1 - Polychlorinated biphenyl 126 affects expression of genes involved in stress-immune interaction in primary cultures of rainbow trout anterior kidney cells

AU - Quabius, E S

AU - Krupp, G

AU - Secombes, C J

PY - 2005

Y1 - 2005

N2 - Stress and immune function are linked in all vertebrates, including teleost fish. Polychlorinated biphenyls (PCBs) are immunotoxic and impair the ability of fish to respond to additional stressors. In this study, we investigated the effects of PCB126 oil stress and immune function and the interaction of these systems in fish using primary Cultures of rainbow trout anterior kidney cells as a model. Gene expression levels of cytochrome P4501A (CYP1A), interleukin-1 beta (1L-1 beta), and glucocorticoid receptor (GR) were measured by real-time quantitative polymerase chain reaction. These genes play important roles in detoxification and immune and stress homeostasis, respectively. Incubation with PCB126 led to increased IL-I beta expression between 30 min and 2 h of exposure, with expression back to basal levels after 6 h. Lipopolysaccharide (LPS) incubation evoked normal IL-1 beta responses after 2 and 24 h PCB incubation. Gene expression levels of GR and CYP1A increased in a time- and dose-dependent manner, reaching a plateau after 12 h of incubation. Preincubation with cortisol resulted in decreased IL-1 beta expression, increased expression of CYP1A and GR, and was accompanied by an abolished PCB responsiveness after more than 4 h of cortisol incubation. We Conclude that PCB 126 exposure is not "stressful," as increased cortisol levels Would result in depressed IL-1 beta expression. Incubation with PCB126 evokes a transient stimulation rather than permanent damage of the immune system, as LPS stimulation resulted in increased IL-1 beta expression after PCB incubation. Prolonged cortisol preincubation, resembling a chronic stress paradigm, negatively affects the immune responsiveness of the cells as well as their capacity for toxicant metabolization.

AB - Stress and immune function are linked in all vertebrates, including teleost fish. Polychlorinated biphenyls (PCBs) are immunotoxic and impair the ability of fish to respond to additional stressors. In this study, we investigated the effects of PCB126 oil stress and immune function and the interaction of these systems in fish using primary Cultures of rainbow trout anterior kidney cells as a model. Gene expression levels of cytochrome P4501A (CYP1A), interleukin-1 beta (1L-1 beta), and glucocorticoid receptor (GR) were measured by real-time quantitative polymerase chain reaction. These genes play important roles in detoxification and immune and stress homeostasis, respectively. Incubation with PCB126 led to increased IL-I beta expression between 30 min and 2 h of exposure, with expression back to basal levels after 6 h. Lipopolysaccharide (LPS) incubation evoked normal IL-1 beta responses after 2 and 24 h PCB incubation. Gene expression levels of GR and CYP1A increased in a time- and dose-dependent manner, reaching a plateau after 12 h of incubation. Preincubation with cortisol resulted in decreased IL-1 beta expression, increased expression of CYP1A and GR, and was accompanied by an abolished PCB responsiveness after more than 4 h of cortisol incubation. We Conclude that PCB 126 exposure is not "stressful," as increased cortisol levels Would result in depressed IL-1 beta expression. Incubation with PCB126 evokes a transient stimulation rather than permanent damage of the immune system, as LPS stimulation resulted in increased IL-1 beta expression after PCB incubation. Prolonged cortisol preincubation, resembling a chronic stress paradigm, negatively affects the immune responsiveness of the cells as well as their capacity for toxicant metabolization.

KW - trout anterior kidney

KW - primary cell culture

KW - stress-immune interaction

KW - polychlorinated biphenyl 126

KW - teleost fish

KW - IN-VIVO

KW - GLUCOCORTICOID-RECEPTOR

KW - ONCORHYNCHUS-MYKISS

KW - CONTAMINATED SITES

KW - CYPRINUS-CARPIO

KW - FISH

KW - CORTISOL

KW - VITRO

KW - INDUCTION

KW - LIPOPOLYSACCHARIDE

M3 - Article

VL - 24

SP - 3053

EP - 3060

JO - Environmental Toxicology and Chemistry

JF - Environmental Toxicology and Chemistry

SN - 0730-7268

ER -