Polymorphic dinucleotide microsatellite loci in the clonal ascidian Diplosoma listerianum: predominance of compound and highly interrupted imperfect repeats.

L. Maclean, A. T. Pardini, Leslie Robert Noble, J. D. D. Bishop, Catherine Sue Jones

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We report the isolation of the first dinucleotide microsatellite loci from the clonal ascidian, Diplosoma listerianum. Most repeats were compound and highly interrupted, with flanking sequences containing many small interspersed repetitive regions. Consequently, most primers detected polymerase chain reaction products outside the expected size range, and only five out of 15 primers detected polymorphic single-locus markers. Characterization of five variable loci from two UK populations revealed three to seven alleles per locus, with observed heterozygosity of 0.00-0.86 and expected heterozygosity of 0.10-0.66. Three loci showed significant heterozygote deficits either because of inbreeding, population substructure or the presence of null alleles.

Original languageEnglish
Pages (from-to)283-285
Number of pages2
JournalMolecular Ecology Notes
Volume4
Issue number2
DOIs
Publication statusPublished - 2004

Keywords

  • clonal ascidian
  • Diplosoma listerianum
  • genetic markers
  • microsatellites

Cite this

Polymorphic dinucleotide microsatellite loci in the clonal ascidian Diplosoma listerianum: predominance of compound and highly interrupted imperfect repeats. / Maclean, L.; Pardini, A. T.; Noble, Leslie Robert; Bishop, J. D. D.; Jones, Catherine Sue.

In: Molecular Ecology Notes, Vol. 4, No. 2, 2004, p. 283-285.

Research output: Contribution to journalArticle

@article{5f60af308be44d5d9a0c01d50b4ac87c,
title = "Polymorphic dinucleotide microsatellite loci in the clonal ascidian Diplosoma listerianum: predominance of compound and highly interrupted imperfect repeats.",
abstract = "We report the isolation of the first dinucleotide microsatellite loci from the clonal ascidian, Diplosoma listerianum. Most repeats were compound and highly interrupted, with flanking sequences containing many small interspersed repetitive regions. Consequently, most primers detected polymerase chain reaction products outside the expected size range, and only five out of 15 primers detected polymorphic single-locus markers. Characterization of five variable loci from two UK populations revealed three to seven alleles per locus, with observed heterozygosity of 0.00-0.86 and expected heterozygosity of 0.10-0.66. Three loci showed significant heterozygote deficits either because of inbreeding, population substructure or the presence of null alleles.",
keywords = "clonal ascidian, Diplosoma listerianum, genetic markers, microsatellites",
author = "L. Maclean and Pardini, {A. T.} and Noble, {Leslie Robert} and Bishop, {J. D. D.} and Jones, {Catherine Sue}",
year = "2004",
doi = "10.1111/j.1471-8286.2004.00643.x",
language = "English",
volume = "4",
pages = "283--285",
journal = "Molecular Ecology Notes",
issn = "1471-8278",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - Polymorphic dinucleotide microsatellite loci in the clonal ascidian Diplosoma listerianum: predominance of compound and highly interrupted imperfect repeats.

AU - Maclean, L.

AU - Pardini, A. T.

AU - Noble, Leslie Robert

AU - Bishop, J. D. D.

AU - Jones, Catherine Sue

PY - 2004

Y1 - 2004

N2 - We report the isolation of the first dinucleotide microsatellite loci from the clonal ascidian, Diplosoma listerianum. Most repeats were compound and highly interrupted, with flanking sequences containing many small interspersed repetitive regions. Consequently, most primers detected polymerase chain reaction products outside the expected size range, and only five out of 15 primers detected polymorphic single-locus markers. Characterization of five variable loci from two UK populations revealed three to seven alleles per locus, with observed heterozygosity of 0.00-0.86 and expected heterozygosity of 0.10-0.66. Three loci showed significant heterozygote deficits either because of inbreeding, population substructure or the presence of null alleles.

AB - We report the isolation of the first dinucleotide microsatellite loci from the clonal ascidian, Diplosoma listerianum. Most repeats were compound and highly interrupted, with flanking sequences containing many small interspersed repetitive regions. Consequently, most primers detected polymerase chain reaction products outside the expected size range, and only five out of 15 primers detected polymorphic single-locus markers. Characterization of five variable loci from two UK populations revealed three to seven alleles per locus, with observed heterozygosity of 0.00-0.86 and expected heterozygosity of 0.10-0.66. Three loci showed significant heterozygote deficits either because of inbreeding, population substructure or the presence of null alleles.

KW - clonal ascidian

KW - Diplosoma listerianum

KW - genetic markers

KW - microsatellites

U2 - 10.1111/j.1471-8286.2004.00643.x

DO - 10.1111/j.1471-8286.2004.00643.x

M3 - Article

VL - 4

SP - 283

EP - 285

JO - Molecular Ecology Notes

JF - Molecular Ecology Notes

SN - 1471-8278

IS - 2

ER -