Polymorphisms in the P2X7 receptor gene are associated with low lumbar spine bone mineral density and accelerated bone loss in post-menopausal women

Alison Gartland, Kristen K. Skarratt, Lynne J. Hocking, Claire Parsons, Leanne Stokes, Niklas Rye Jorgensen, William D. Fraser, David M. Reid, James A. Gallagher, James S. Wiley

Research output: Contribution to journalArticle

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Abstract

The P2X7 receptor gene (P2RX7) is highly polymorphic with five previously described loss-of-function (LOF) single-nucleotide polymorphisms (SNP; c.151+1G>T, c.946G>A, c.1096C>G, c.1513A>C and c.1729T>A) and one gain-of-function SNP (c.489C>T). The purpose of this study was to determine whether the functional P2RX7 SNPs are associated with lumbar spine (LS) bone mineral density (BMD), a key determinant of vertebral fracture risk, in post-menopausal women. We genotyped 506 post-menopausal women from the Aberdeen Prospective Osteoporosis Screening Study (APOSS) for the above SNPs. Lumbar spine BMD was measured at baseline and at 6-7 year follow-up. P2RX7 genotyping was performed by homogeneous mass extension. We found association of c.946A (p.Arg307Gln) with lower LS-BMD at baseline (P=0.004, beta=-0.12) and follow-up (P=0.002, beta=-0.13). Further analysis showed that a combined group of subjects who had LOF SNPs (n=48) had nearly ninefold greater annualised percent change in LS-BMD than subjects who were wild type at the six SNP positions (n=84; rate of loss=-0.94%/year and -0.11%/year, respectively, P=0.0005, unpaired t-test). This is the first report that describes association of the c.946A (p.Arg307Gln) LOF SNP with low LS-BMD, and that other LOF SNPs, which result in reduced or no function of the P2X7 receptor, may contribute to accelerated bone loss. Certain polymorphic variants of P2RX7 may identify women at greater risk of developing osteoporosis. European Journal of Human Genetics (2012) 20, 559-564; doi:10.1038/ejhg.2011.245; published online 11 January 2012

Original languageEnglish
Pages (from-to)559-564
Number of pages6
JournalEJHG : European journal of human genetics : the official journal of the European Society of Human Genetics.
Volume20
Issue number5
DOIs
Publication statusPublished - May 2012

Keywords

  • P2RX7
  • LS-BMD
  • single-nucleotide polymorphisms
  • human P2X(7) receptor
  • protein-kinase-C
  • lysophosphatidic acid
  • in-vitro
  • regional osteoperosis
  • recruitment methods
  • screening programs
  • osteoblatic cells
  • binding-site
  • kappa-B

Cite this

Polymorphisms in the P2X7 receptor gene are associated with low lumbar spine bone mineral density and accelerated bone loss in post-menopausal women. / Gartland, Alison; Skarratt, Kristen K.; Hocking, Lynne J.; Parsons, Claire; Stokes, Leanne; Jorgensen, Niklas Rye; Fraser, William D.; Reid, David M.; Gallagher, James A.; Wiley, James S.

In: EJHG : European journal of human genetics : the official journal of the European Society of Human Genetics. , Vol. 20, No. 5, 05.2012, p. 559-564.

Research output: Contribution to journalArticle

Gartland, Alison ; Skarratt, Kristen K. ; Hocking, Lynne J. ; Parsons, Claire ; Stokes, Leanne ; Jorgensen, Niklas Rye ; Fraser, William D. ; Reid, David M. ; Gallagher, James A. ; Wiley, James S. / Polymorphisms in the P2X7 receptor gene are associated with low lumbar spine bone mineral density and accelerated bone loss in post-menopausal women. In: EJHG : European journal of human genetics : the official journal of the European Society of Human Genetics. . 2012 ; Vol. 20, No. 5. pp. 559-564.
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AU - Gartland, Alison

AU - Skarratt, Kristen K.

AU - Hocking, Lynne J.

AU - Parsons, Claire

AU - Stokes, Leanne

AU - Jorgensen, Niklas Rye

AU - Fraser, William D.

AU - Reid, David M.

AU - Gallagher, James A.

AU - Wiley, James S.

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AB - The P2X7 receptor gene (P2RX7) is highly polymorphic with five previously described loss-of-function (LOF) single-nucleotide polymorphisms (SNP; c.151+1G>T, c.946G>A, c.1096C>G, c.1513A>C and c.1729T>A) and one gain-of-function SNP (c.489C>T). The purpose of this study was to determine whether the functional P2RX7 SNPs are associated with lumbar spine (LS) bone mineral density (BMD), a key determinant of vertebral fracture risk, in post-menopausal women. We genotyped 506 post-menopausal women from the Aberdeen Prospective Osteoporosis Screening Study (APOSS) for the above SNPs. Lumbar spine BMD was measured at baseline and at 6-7 year follow-up. P2RX7 genotyping was performed by homogeneous mass extension. We found association of c.946A (p.Arg307Gln) with lower LS-BMD at baseline (P=0.004, beta=-0.12) and follow-up (P=0.002, beta=-0.13). Further analysis showed that a combined group of subjects who had LOF SNPs (n=48) had nearly ninefold greater annualised percent change in LS-BMD than subjects who were wild type at the six SNP positions (n=84; rate of loss=-0.94%/year and -0.11%/year, respectively, P=0.0005, unpaired t-test). This is the first report that describes association of the c.946A (p.Arg307Gln) LOF SNP with low LS-BMD, and that other LOF SNPs, which result in reduced or no function of the P2X7 receptor, may contribute to accelerated bone loss. Certain polymorphic variants of P2RX7 may identify women at greater risk of developing osteoporosis. European Journal of Human Genetics (2012) 20, 559-564; doi:10.1038/ejhg.2011.245; published online 11 January 2012

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KW - LS-BMD

KW - single-nucleotide polymorphisms

KW - human P2X(7) receptor

KW - protein-kinase-C

KW - lysophosphatidic acid

KW - in-vitro

KW - regional osteoperosis

KW - recruitment methods

KW - screening programs

KW - osteoblatic cells

KW - binding-site

KW - kappa-B

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M3 - Article

VL - 20

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EP - 564

JO - EJHG : European journal of human genetics : the official journal of the European Society of Human Genetics.

JF - EJHG : European journal of human genetics : the official journal of the European Society of Human Genetics.

SN - 1018-4813

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