Polyphosphate delays fibrin polymerisation and alters the mechanical properties of the fibrin network

C. S. Whyte, Irina N. Chernysh, Marco M. Domingues, Simon Connell, John W. Weisel, Robert A S Ariëns, Nicola J. Mutch

Research output: Contribution to journalArticle

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Abstract

Polyphosphate (polyP) binds to fibrin(ogen) and alters fibrin structure, generating a heterogeneous network composed of ‘knots’ interspersed by large pores. Here we show platelet-derived polyP elicits similar structural changes in fibrin and examine the mechanism by which polyP alters fibrin structure. Polymerisation of fibrinogen with thrombin and CaCl2 was studied using spinning disk confocal (SDC) microscopy. PolyP delayed fibrin polymerisation generating shorter protofibrils emanating from a nucleus-type structure. Consistent with this, cascade blue-polyP accumulated in fibrin ‘knots’. Protofibril formation was visualized by atomic force microscopy (AFM) ±polyP. In the presence of polyP abundant monomers of longer length were visualized by AFM, suggesting that polyP binds to monomeric fibrin. Shorter oligomers form in the presence of polyP, consistent with the stunted protofibrils visualized by SDC microscopy. We examined whether these structural changes induced by polyP alter fibrin’s viscoelastic properties by rheometry. PolyP reduced the stiffness (G’) and
ability of the fibrin network to deform plastically G’’, but to different extents.
Consequently, the relative plastic component (loss tangent (G’’/G’)) was 61% higher implying that networks containing polyP are less stiff and more plastic. Local rheological measurements, performed using magnetic tweezers, indicate that the fibrin dense knots are stiffer and more plastic, reflecting the heterogeneity of the network. Our data show that polyP impedes fibrin polymerisation, stunting protofibril growth producing ‘knotted’ regions, which are rich in fibrin and polyP. Consequently, the mechanical properties of the fibrin network are altered resulting in clots with overall reduced stiffness and increased ability to deform plastically.
Original languageEnglish
Pages (from-to)897-903
Number of pages7
JournalThrombosis and Haemostasis
Volume116
Issue number5
Early online date25 Aug 2016
DOIs
Publication statusPublished - Nov 2016

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Polyphosphates
Fibrin
Polymerization
Plastics
Atomic Force Microscopy
Polyps
Confocal Microscopy
Growth Disorders
Thrombin
Fibrinogen

Keywords

  • Fibrin(ogen)
  • polyphosphate
  • polymerisation
  • fibrin
  • clot structure

Cite this

Polyphosphate delays fibrin polymerisation and alters the mechanical properties of the fibrin network. / Whyte, C. S.; Chernysh, Irina N. ; Domingues, Marco M. ; Connell, Simon ; Weisel, John W.; Ariëns, Robert A S; Mutch, Nicola J.

In: Thrombosis and Haemostasis, Vol. 116, No. 5, 11.2016, p. 897-903.

Research output: Contribution to journalArticle

Whyte, C. S. ; Chernysh, Irina N. ; Domingues, Marco M. ; Connell, Simon ; Weisel, John W. ; Ariëns, Robert A S ; Mutch, Nicola J. / Polyphosphate delays fibrin polymerisation and alters the mechanical properties of the fibrin network. In: Thrombosis and Haemostasis. 2016 ; Vol. 116, No. 5. pp. 897-903.
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T1 - Polyphosphate delays fibrin polymerisation and alters the mechanical properties of the fibrin network

AU - Whyte, C. S.

AU - Chernysh, Irina N.

AU - Domingues, Marco M.

AU - Connell, Simon

AU - Weisel, John W.

AU - Ariëns, Robert A S

AU - Mutch, Nicola J.

N1 - This research was supported by grants FS/11/2/28579 (NJM) and PG/11/1/28461 (NJM, CSW & RASA) from the British Heart Foundation and NIH HL090774 (JWW). Travel for this work was supported by a Scottish Universities Life Science Alliance exchange grant (CSW).

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N2 - Polyphosphate (polyP) binds to fibrin(ogen) and alters fibrin structure, generating a heterogeneous network composed of ‘knots’ interspersed by large pores. Here we show platelet-derived polyP elicits similar structural changes in fibrin and examine the mechanism by which polyP alters fibrin structure. Polymerisation of fibrinogen with thrombin and CaCl2 was studied using spinning disk confocal (SDC) microscopy. PolyP delayed fibrin polymerisation generating shorter protofibrils emanating from a nucleus-type structure. Consistent with this, cascade blue-polyP accumulated in fibrin ‘knots’. Protofibril formation was visualized by atomic force microscopy (AFM) ±polyP. In the presence of polyP abundant monomers of longer length were visualized by AFM, suggesting that polyP binds to monomeric fibrin. Shorter oligomers form in the presence of polyP, consistent with the stunted protofibrils visualized by SDC microscopy. We examined whether these structural changes induced by polyP alter fibrin’s viscoelastic properties by rheometry. PolyP reduced the stiffness (G’) andability of the fibrin network to deform plastically G’’, but to different extents.Consequently, the relative plastic component (loss tangent (G’’/G’)) was 61% higher implying that networks containing polyP are less stiff and more plastic. Local rheological measurements, performed using magnetic tweezers, indicate that the fibrin dense knots are stiffer and more plastic, reflecting the heterogeneity of the network. Our data show that polyP impedes fibrin polymerisation, stunting protofibril growth producing ‘knotted’ regions, which are rich in fibrin and polyP. Consequently, the mechanical properties of the fibrin network are altered resulting in clots with overall reduced stiffness and increased ability to deform plastically.

AB - Polyphosphate (polyP) binds to fibrin(ogen) and alters fibrin structure, generating a heterogeneous network composed of ‘knots’ interspersed by large pores. Here we show platelet-derived polyP elicits similar structural changes in fibrin and examine the mechanism by which polyP alters fibrin structure. Polymerisation of fibrinogen with thrombin and CaCl2 was studied using spinning disk confocal (SDC) microscopy. PolyP delayed fibrin polymerisation generating shorter protofibrils emanating from a nucleus-type structure. Consistent with this, cascade blue-polyP accumulated in fibrin ‘knots’. Protofibril formation was visualized by atomic force microscopy (AFM) ±polyP. In the presence of polyP abundant monomers of longer length were visualized by AFM, suggesting that polyP binds to monomeric fibrin. Shorter oligomers form in the presence of polyP, consistent with the stunted protofibrils visualized by SDC microscopy. We examined whether these structural changes induced by polyP alter fibrin’s viscoelastic properties by rheometry. PolyP reduced the stiffness (G’) andability of the fibrin network to deform plastically G’’, but to different extents.Consequently, the relative plastic component (loss tangent (G’’/G’)) was 61% higher implying that networks containing polyP are less stiff and more plastic. Local rheological measurements, performed using magnetic tweezers, indicate that the fibrin dense knots are stiffer and more plastic, reflecting the heterogeneity of the network. Our data show that polyP impedes fibrin polymerisation, stunting protofibril growth producing ‘knotted’ regions, which are rich in fibrin and polyP. Consequently, the mechanical properties of the fibrin network are altered resulting in clots with overall reduced stiffness and increased ability to deform plastically.

KW - Fibrin(ogen)

KW - polyphosphate

KW - polymerisation

KW - fibrin

KW - clot structure

U2 - 10.1160/TH16-01-0062

DO - 10.1160/TH16-01-0062

M3 - Article

VL - 116

SP - 897

EP - 903

JO - Thrombosis and Haemostasis

JF - Thrombosis and Haemostasis

SN - 0340-6245

IS - 5

ER -