Postprandial hepatic protein expression in trout Oncorhynchus mykiss a proteomics examination

Elena Mente, Graham J. Pierce, Efthimia Antonopoulou, David Andrew Stead, SAM Martin

Research output: Contribution to journalArticle

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5 Downloads (Pure)

Abstract

Following a meal, a series of physiological changes occurs in animals as they digest, absorb and assimilate ingested nutrients, the kinetics of these responses depends on metabolic rate and nutrient quality. Here we investigated the hepatic proteome in the ectothermic teleost, the rainbow trout, following a single meal to define the postprandial expression of hepatic proteins. The fish were fed a high marine fishmeal/fish oil single meal following a period of 24 h without feeding. Liver protein profiles were examined by 2D gel electrophoresis just before feeding (time 0 h) and at 6 and 12 h after feeding. Of a total of 588 protein spots analysed in a temporal fashion, 49 differed significantly in abundance between the three time groups (ANOVA, p<0.05), before and after feeding, 15 were increased and 34 were decreased in abundance after feeding. Amino acid metabolism-regulated proteins such as phenylalanine-4-hydroxylase and proliferating cell antigen were increased in abundance 12 and 6 h following the meal, suggesting by this time that the fish were increasing their protein turnover to utilize efficiently their dietary protein consumption. Overall, these results highlight some specificity of the trout metabolism and identify postprandial response of metabolism-related proteins 6–12 h after feeding a single meal.
Original languageEnglish
Pages (from-to)79-85
Number of pages7
JournalBiochemistry and Biophysics Reports
Volume9
Early online date4 Nov 2016
DOIs
Publication statusPublished - Mar 2017

Fingerprint

Trout
Oncorhynchus mykiss
Proteomics
Meals
Liver
Metabolism
Proteins
Fish
Nutrients
Fishes
Phenylalanine Hydroxylase
Food
Dietary Proteins
Fish Oils
Electrophoresis, Gel, Two-Dimensional
Proteome
Analysis of variance (ANOVA)
Electrophoresis
Analysis of Variance
Animals

Keywords

  • postprandial
  • hepatic proteins
  • trout
  • proteomics
  • diet
  • meal
  • nutrition

Cite this

Postprandial hepatic protein expression in trout Oncorhynchus mykiss a proteomics examination. / Mente, Elena; Pierce, Graham J.; Antonopoulou, Efthimia ; Stead, David Andrew; Martin, SAM.

In: Biochemistry and Biophysics Reports , Vol. 9, 03.2017, p. 79-85.

Research output: Contribution to journalArticle

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