Potential of the electrical conductivity of seed soak water and early counts of radicle emergence to assess seed quality in some native species

Maria Marin, Giles Laverack, Alison Anne Powell, Stan Matthews

Research output: Contribution to journalArticle

Abstract

The potential of the electrical conductivity (EC) test to predict final germination was evaluated in seed lots from seven native species. In four of the seven species tested (Cyanus segetum, Prunella vulgaris, Valeriana officinalis and Centaurea nigra), EC was indicative of the final germination (radicle emergence; RE), with high levels of leakage seen for lots with low germination. Single seed measurements of solute leakage from two species confirmed the link between high leakage and the failure to germinate, while in Cyanus segetum, high EC was also associated with slow germination (after 42 hours). Reduced EC and earlier RE following a pre-hydration treatment in C. segetum supported the hypothesis that metabolic repair may occur during early imbibition. A single early count of RE (at 42 hours) also predicted germination (R 2 ≥ 0.858) for 12 seed lots of C. segetum. Therefore, both measurements of solute leakage from seeds using EC and early counts of RE have potential to predict the germination of seed lots from native species. The use of the EC test may be dependent on the structure of the seed, but the RE test may be applicable to a wider range of species and predict both germination and vigour differences.
Original languageEnglish
Pages (from-to)71-86
Number of pages16
JournalSeed Science and Technology
Volume46
Issue number1
DOIs
Publication statusPublished - 1 Apr 2018

Fingerprint

seed quality
electrical conductivity
indigenous species
germination
seeds
Centaurea
water
solutes
Centaurea nigra
Prunella vulgaris
Valeriana officinalis
testing
imbibition
vigor
seed germination

Keywords

  • electrical conductivity
  • embryo development
  • native seed
  • radicle emergence
  • seed germination
  • seed quality
  • seed vigour

Cite this

Potential of the electrical conductivity of seed soak water and early counts of radicle emergence to assess seed quality in some native species. / Marin, Maria; Laverack, Giles; Powell, Alison Anne; Matthews, Stan.

In: Seed Science and Technology, Vol. 46, No. 1, 01.04.2018, p. 71-86.

Research output: Contribution to journalArticle

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abstract = "The potential of the electrical conductivity (EC) test to predict final germination was evaluated in seed lots from seven native species. In four of the seven species tested (Cyanus segetum, Prunella vulgaris, Valeriana officinalis and Centaurea nigra), EC was indicative of the final germination (radicle emergence; RE), with high levels of leakage seen for lots with low germination. Single seed measurements of solute leakage from two species confirmed the link between high leakage and the failure to germinate, while in Cyanus segetum, high EC was also associated with slow germination (after 42 hours). Reduced EC and earlier RE following a pre-hydration treatment in C. segetum supported the hypothesis that metabolic repair may occur during early imbibition. A single early count of RE (at 42 hours) also predicted germination (R 2 ≥ 0.858) for 12 seed lots of C. segetum. Therefore, both measurements of solute leakage from seeds using EC and early counts of RE have potential to predict the germination of seed lots from native species. The use of the EC test may be dependent on the structure of the seed, but the RE test may be applicable to a wider range of species and predict both germination and vigour differences.",
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N1 - The research leading to these results has received funding from the People Programme (Marie Curie Actions) of the European Union’s Seventh Framework Programme FP7/2007- 2013/ under REA grant agreement n°607785 (NASSTEC). We thank Pietro Iannetta, Sean Chapman and Kathryn Wright from the James Hutton Institute in Dundee for their assistance in seed internal morphology assessments. We thank Peter Toorop and Cristina Blandino for their valuable suggestions.

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