Potentiation by metal ions of the efficacy of the ionophores, monensin and tetronasin, towards four species of ruminal bacteria

C. James Newbold, R. John Wallace, Nicola D. Walker-Bax

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Concentrations of Na(+), K(+) and Ca(2+) in the growth medium were varied within limits normally found in vivo to determine how cation concentrations affect the sensitivity of ruminal bacteria to the ionophores, monensin (a Na(+)/H(+) and K(+)/H(+) exchanger) and tetronasin (Ca(2+)/H(+)). High [Na(+)] (172 mM cf. 137 mM in control medium) enhanced the efficacy of monensin towards Eubacterium ruminantium 2388, Streptococcus bovis C277, Lactobacillus casei LB17 and Prevotella albensis M384. High [K(+)] (35 mM cf. 19 mM) alone caused a decreased potency of both ionophores, except with L. casei. Added Ca(2+) (7.4 cf. 2.8 mM) increased the potency of tetronasin when [Na(+)] was low. High [Na(+)] alone also potentiated the efficacy of tetronasin. Monensin caused intracellular [Na(+)] and [K(+)] to be decreased in the most sensitive of these organisms, E. ruminantium, whereas only intracellular [Ca(2+)] fell with tetronasin. The changes were small; however, Deltap fell by only 20 mV after 2 h when ionophores caused immediate cessation of growth. ATP concentrations fell by 77% and 75% with monensin and tetronasin, respectively. Thus, altering cation concentrations might be used to potentiate the efficacy of ionophores, by increasing the rate of energy expenditure to maintain ionic homoeostasis in sensitive bacteria
Original languageEnglish
Pages (from-to)161-167
Number of pages7
JournalFEMS Microbiology Letters
Volume338
Issue number2
Early online date4 Dec 2012
DOIs
Publication statusPublished - Jan 2013

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Monensin
Ionophores
Metals
Ions
Bacteria
Lactobacillus casei
Ruminants
Potassium-Hydrogen Antiporters
Cations
Streptococcus bovis
Prevotella
Eubacterium
Growth
Energy Metabolism
Homeostasis
Adenosine Triphosphate
tetronasin

Keywords

  • ionophore
  • monensin
  • ruminal bacteria
  • tetronasin

Cite this

Potentiation by metal ions of the efficacy of the ionophores, monensin and tetronasin, towards four species of ruminal bacteria. / Newbold, C. James; Wallace, R. John; Walker-Bax, Nicola D. .

In: FEMS Microbiology Letters, Vol. 338, No. 2, 01.2013, p. 161-167.

Research output: Contribution to journalArticle

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