Prediction of [3-C-14] phenyldodecane biodegradation in cable insulating oil-spiked soil using selected extraction techniques

N. Dew, Graeme Iain Paton, K. T. Semple

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16 Citations (Scopus)

Abstract

This study investigated the use of an aqueous hydroxypropyl-p-cyclodextrin (HPCD) shake extraction in predicting microbial mineralisation and total loss of [3-C-14]phenyldodecane associated activity in soils spiked with cable insulating oil; phenyldodecane represents a major constituent of cable insulating oil. Direct comparisons were made between freshly spiked and aged soils, and following composting. Soil was spiked with [3-C-14]phenyldodecane (10 mg kg(-1)) and stored in microcosms and aged for 1, 23, 44, 65, 90 and 153 d. At each sample time point, a variety of analyses were performed to assess the relationship between chemical and biological techniques in determining mineralisation and loss of C-14-activity in soils under composting and non-composting conditions. Methods included determination of total C-14-activity remaining, dichloromethane (DCM) and HPCD extractions. Mineralisation assays were also carried out to quantify the fraction of C-14-phenyldodecane associated activity available for degradation in the soil at each time point. DCM and HPCD extractability were compared to contaminant mineralisation and to totalloss of C-14-phenyldodecane associated activity from the microcosms, after 153 d incubation. Poor relationships were found between (i) the amount of C-14-activity mineralised and the fraction removed from the soils using DCM extraction and (ii) DCM extraction and total loss of [C-14]phenyldodecane associated activity from the soil systems. Good relationships were observed between (i) the amount of C-14-activity mineralised and the fraction removed from the soils using the HPCD extraction and (ii) HPCD extraction and total loss Of [C-14] phenyldodecane associated activity from the soil systems. The results of this study indicate that an aqueous HPCD extraction may be a useful tool in assessing the microbial availability of phenyldodecane in freshly and aged spiked soils. (c) 2005 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)316-323
Number of pages8
JournalEnvironmental Pollution
Volume138
DOIs
Publication statusPublished - 2005

Keywords

  • cyclodextrin
  • total contaminant loss
  • bioavailability
  • mineralisation
  • phenyldodecane
  • HYDROXYPROPYL-BETA-CYCLODEXTRIN
  • HYDROPHOBIC ORGANIC-COMPOUNDS
  • PAH BIOAVAILABILITY
  • CONTAMINATED SOILS
  • PHENANTHRENE
  • CHEMICALS
  • SEDIMENTS
  • EXTRACTABILITY
  • SEQUESTRATION
  • POLLUTANTS

Cite this

@article{e7018103c3784e8ab90506f92dff258b,
title = "Prediction of [3-C-14] phenyldodecane biodegradation in cable insulating oil-spiked soil using selected extraction techniques",
abstract = "This study investigated the use of an aqueous hydroxypropyl-p-cyclodextrin (HPCD) shake extraction in predicting microbial mineralisation and total loss of [3-C-14]phenyldodecane associated activity in soils spiked with cable insulating oil; phenyldodecane represents a major constituent of cable insulating oil. Direct comparisons were made between freshly spiked and aged soils, and following composting. Soil was spiked with [3-C-14]phenyldodecane (10 mg kg(-1)) and stored in microcosms and aged for 1, 23, 44, 65, 90 and 153 d. At each sample time point, a variety of analyses were performed to assess the relationship between chemical and biological techniques in determining mineralisation and loss of C-14-activity in soils under composting and non-composting conditions. Methods included determination of total C-14-activity remaining, dichloromethane (DCM) and HPCD extractions. Mineralisation assays were also carried out to quantify the fraction of C-14-phenyldodecane associated activity available for degradation in the soil at each time point. DCM and HPCD extractability were compared to contaminant mineralisation and to totalloss of C-14-phenyldodecane associated activity from the microcosms, after 153 d incubation. Poor relationships were found between (i) the amount of C-14-activity mineralised and the fraction removed from the soils using DCM extraction and (ii) DCM extraction and total loss of [C-14]phenyldodecane associated activity from the soil systems. Good relationships were observed between (i) the amount of C-14-activity mineralised and the fraction removed from the soils using the HPCD extraction and (ii) HPCD extraction and total loss Of [C-14] phenyldodecane associated activity from the soil systems. The results of this study indicate that an aqueous HPCD extraction may be a useful tool in assessing the microbial availability of phenyldodecane in freshly and aged spiked soils. (c) 2005 Elsevier Ltd. All rights reserved.",
keywords = "cyclodextrin, total contaminant loss, bioavailability, mineralisation, phenyldodecane, HYDROXYPROPYL-BETA-CYCLODEXTRIN, HYDROPHOBIC ORGANIC-COMPOUNDS, PAH BIOAVAILABILITY, CONTAMINATED SOILS, PHENANTHRENE, CHEMICALS, SEDIMENTS, EXTRACTABILITY, SEQUESTRATION, POLLUTANTS",
author = "N. Dew and Paton, {Graeme Iain} and Semple, {K. T.}",
year = "2005",
doi = "10.1016/j.envpol.2005.03.009",
language = "English",
volume = "138",
pages = "316--323",
journal = "Environmental Pollution",
issn = "0269-7491",
publisher = "ELSEVIER APPL SCI PUBL LTD",

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TY - JOUR

T1 - Prediction of [3-C-14] phenyldodecane biodegradation in cable insulating oil-spiked soil using selected extraction techniques

AU - Dew, N.

AU - Paton, Graeme Iain

AU - Semple, K. T.

PY - 2005

Y1 - 2005

N2 - This study investigated the use of an aqueous hydroxypropyl-p-cyclodextrin (HPCD) shake extraction in predicting microbial mineralisation and total loss of [3-C-14]phenyldodecane associated activity in soils spiked with cable insulating oil; phenyldodecane represents a major constituent of cable insulating oil. Direct comparisons were made between freshly spiked and aged soils, and following composting. Soil was spiked with [3-C-14]phenyldodecane (10 mg kg(-1)) and stored in microcosms and aged for 1, 23, 44, 65, 90 and 153 d. At each sample time point, a variety of analyses were performed to assess the relationship between chemical and biological techniques in determining mineralisation and loss of C-14-activity in soils under composting and non-composting conditions. Methods included determination of total C-14-activity remaining, dichloromethane (DCM) and HPCD extractions. Mineralisation assays were also carried out to quantify the fraction of C-14-phenyldodecane associated activity available for degradation in the soil at each time point. DCM and HPCD extractability were compared to contaminant mineralisation and to totalloss of C-14-phenyldodecane associated activity from the microcosms, after 153 d incubation. Poor relationships were found between (i) the amount of C-14-activity mineralised and the fraction removed from the soils using DCM extraction and (ii) DCM extraction and total loss of [C-14]phenyldodecane associated activity from the soil systems. Good relationships were observed between (i) the amount of C-14-activity mineralised and the fraction removed from the soils using the HPCD extraction and (ii) HPCD extraction and total loss Of [C-14] phenyldodecane associated activity from the soil systems. The results of this study indicate that an aqueous HPCD extraction may be a useful tool in assessing the microbial availability of phenyldodecane in freshly and aged spiked soils. (c) 2005 Elsevier Ltd. All rights reserved.

AB - This study investigated the use of an aqueous hydroxypropyl-p-cyclodextrin (HPCD) shake extraction in predicting microbial mineralisation and total loss of [3-C-14]phenyldodecane associated activity in soils spiked with cable insulating oil; phenyldodecane represents a major constituent of cable insulating oil. Direct comparisons were made between freshly spiked and aged soils, and following composting. Soil was spiked with [3-C-14]phenyldodecane (10 mg kg(-1)) and stored in microcosms and aged for 1, 23, 44, 65, 90 and 153 d. At each sample time point, a variety of analyses were performed to assess the relationship between chemical and biological techniques in determining mineralisation and loss of C-14-activity in soils under composting and non-composting conditions. Methods included determination of total C-14-activity remaining, dichloromethane (DCM) and HPCD extractions. Mineralisation assays were also carried out to quantify the fraction of C-14-phenyldodecane associated activity available for degradation in the soil at each time point. DCM and HPCD extractability were compared to contaminant mineralisation and to totalloss of C-14-phenyldodecane associated activity from the microcosms, after 153 d incubation. Poor relationships were found between (i) the amount of C-14-activity mineralised and the fraction removed from the soils using DCM extraction and (ii) DCM extraction and total loss of [C-14]phenyldodecane associated activity from the soil systems. Good relationships were observed between (i) the amount of C-14-activity mineralised and the fraction removed from the soils using the HPCD extraction and (ii) HPCD extraction and total loss Of [C-14] phenyldodecane associated activity from the soil systems. The results of this study indicate that an aqueous HPCD extraction may be a useful tool in assessing the microbial availability of phenyldodecane in freshly and aged spiked soils. (c) 2005 Elsevier Ltd. All rights reserved.

KW - cyclodextrin

KW - total contaminant loss

KW - bioavailability

KW - mineralisation

KW - phenyldodecane

KW - HYDROXYPROPYL-BETA-CYCLODEXTRIN

KW - HYDROPHOBIC ORGANIC-COMPOUNDS

KW - PAH BIOAVAILABILITY

KW - CONTAMINATED SOILS

KW - PHENANTHRENE

KW - CHEMICALS

KW - SEDIMENTS

KW - EXTRACTABILITY

KW - SEQUESTRATION

KW - POLLUTANTS

U2 - 10.1016/j.envpol.2005.03.009

DO - 10.1016/j.envpol.2005.03.009

M3 - Article

VL - 138

SP - 316

EP - 323

JO - Environmental Pollution

JF - Environmental Pollution

SN - 0269-7491

ER -