Preliminary characterisation of locust diuretic peptide (DP-1) and another corpus cardiacum peptide (LCCP)

P. J. Morgan; K. J. Siegert; W. Mordue

doi:10.1016/0020-1790(87)90082-5

Preliminary characterisation of locust diuretic peptide (DP-1) and another corpus cardiacum peptide (LCCP)

P. J. Morgan^*, K. J. Siegert, W. Mordue

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

A simple two step HPLC purification protocol is described for one of the locust diuretic peptides (DP-1) and a second corpus cardiacum peptide (LCCP) of unknown function. DP-1 and LCCP are extracted from corpora cardiaca in 20% aqueous methanol (v/v), and applied to a high performance size-exclusion chromatography (HPSEC) column, from which DP-1 and LCCP co-elute in a fraction designated F7/8; this corresponds to a relative molecular mass of ca 6000-7000. Without sample concentration, F7/8 (2 ml) is applied directly onto a wide-pore reversed-phase HPLC column; LCCP and DP-1 are eluted in ca 12 and 20 min respectively on an increasing linear gradient of 0.5% min, starting at 30% acetonitrile. The diuretic activity of the DP-1 material was confirmed using the cAMP assay (Morgan and Mordue, 1985a, Insect Biochem. 15, 247-257). DP-1 and LCCP were isolated from ca 650 locusts using the above procedures for sequence analysis by gas-phase sequencing. The low amount of DP-1 purified allowed only a partial and fragmentary sequence to be determined; insufficient material was available for amino acid analysis. The higher amount of LCCP allowed a partial sequence determination of residues 1-40. The titre of DP-1 in locust CC is calculated to be 1.7-2.1 pmol/CC.

Original language	English
Pages (from-to)	383-388
Number of pages	6
Journal	Insect Biochemistry
Volume	17
Issue number	2
DOIs	https://doi.org/10.1016/0020-1790(87)90082-5
Publication status	Published - 1 Jan 1987

Keywords

Diuretic peptide
fluid secretion
gas phase sequencing
HPLC purification
locust corpus cardiacum
partial sequence

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10.1016/0020-1790(87)90082-5

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title = "Preliminary characterisation of locust diuretic peptide (DP-1) and another corpus cardiacum peptide (LCCP)",

abstract = "A simple two step HPLC purification protocol is described for one of the locust diuretic peptides (DP-1) and a second corpus cardiacum peptide (LCCP) of unknown function. DP-1 and LCCP are extracted from corpora cardiaca in 20% aqueous methanol (v/v), and applied to a high performance size-exclusion chromatography (HPSEC) column, from which DP-1 and LCCP co-elute in a fraction designated F7/8; this corresponds to a relative molecular mass of ca 6000-7000. Without sample concentration, F7/8 (2 ml) is applied directly onto a wide-pore reversed-phase HPLC column; LCCP and DP-1 are eluted in ca 12 and 20 min respectively on an increasing linear gradient of 0.5% min, starting at 30% acetonitrile. The diuretic activity of the DP-1 material was confirmed using the cAMP assay (Morgan and Mordue, 1985a, Insect Biochem. 15, 247-257). DP-1 and LCCP were isolated from ca 650 locusts using the above procedures for sequence analysis by gas-phase sequencing. The low amount of DP-1 purified allowed only a partial and fragmentary sequence to be determined; insufficient material was available for amino acid analysis. The higher amount of LCCP allowed a partial sequence determination of residues 1-40. The titre of DP-1 in locust CC is calculated to be 1.7-2.1 pmol/CC.",

keywords = "Diuretic peptide, fluid secretion, gas phase sequencing, HPLC purification, locust corpus cardiacum, partial sequence",

author = "Morgan, {P. J.} and Siegert, {K. J.} and W. Mordue",

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T1 - Preliminary characterisation of locust diuretic peptide (DP-1) and another corpus cardiacum peptide (LCCP)

AU - Morgan, P. J.

AU - Siegert, K. J.

AU - Mordue, W.

PY - 1987/1/1

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N2 - A simple two step HPLC purification protocol is described for one of the locust diuretic peptides (DP-1) and a second corpus cardiacum peptide (LCCP) of unknown function. DP-1 and LCCP are extracted from corpora cardiaca in 20% aqueous methanol (v/v), and applied to a high performance size-exclusion chromatography (HPSEC) column, from which DP-1 and LCCP co-elute in a fraction designated F7/8; this corresponds to a relative molecular mass of ca 6000-7000. Without sample concentration, F7/8 (2 ml) is applied directly onto a wide-pore reversed-phase HPLC column; LCCP and DP-1 are eluted in ca 12 and 20 min respectively on an increasing linear gradient of 0.5% min, starting at 30% acetonitrile. The diuretic activity of the DP-1 material was confirmed using the cAMP assay (Morgan and Mordue, 1985a, Insect Biochem. 15, 247-257). DP-1 and LCCP were isolated from ca 650 locusts using the above procedures for sequence analysis by gas-phase sequencing. The low amount of DP-1 purified allowed only a partial and fragmentary sequence to be determined; insufficient material was available for amino acid analysis. The higher amount of LCCP allowed a partial sequence determination of residues 1-40. The titre of DP-1 in locust CC is calculated to be 1.7-2.1 pmol/CC.

AB - A simple two step HPLC purification protocol is described for one of the locust diuretic peptides (DP-1) and a second corpus cardiacum peptide (LCCP) of unknown function. DP-1 and LCCP are extracted from corpora cardiaca in 20% aqueous methanol (v/v), and applied to a high performance size-exclusion chromatography (HPSEC) column, from which DP-1 and LCCP co-elute in a fraction designated F7/8; this corresponds to a relative molecular mass of ca 6000-7000. Without sample concentration, F7/8 (2 ml) is applied directly onto a wide-pore reversed-phase HPLC column; LCCP and DP-1 are eluted in ca 12 and 20 min respectively on an increasing linear gradient of 0.5% min, starting at 30% acetonitrile. The diuretic activity of the DP-1 material was confirmed using the cAMP assay (Morgan and Mordue, 1985a, Insect Biochem. 15, 247-257). DP-1 and LCCP were isolated from ca 650 locusts using the above procedures for sequence analysis by gas-phase sequencing. The low amount of DP-1 purified allowed only a partial and fragmentary sequence to be determined; insufficient material was available for amino acid analysis. The higher amount of LCCP allowed a partial sequence determination of residues 1-40. The titre of DP-1 in locust CC is calculated to be 1.7-2.1 pmol/CC.

KW - Diuretic peptide

KW - fluid secretion

KW - gas phase sequencing

KW - HPLC purification

KW - locust corpus cardiacum

KW - partial sequence

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DO - 10.1016/0020-1790(87)90082-5

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SN - 0020-1790

VL - 17

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JO - Insect Biochemistry

JF - Insect Biochemistry

IS - 2

ER -

Preliminary characterisation of locust diuretic peptide (DP-1) and another corpus cardiacum peptide (LCCP)

Abstract

Keywords

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