Preparation of hydrogen from water by reduction with lithium aluminium hydride for the analysis of delta H-2 by isotope ratio mass spectrometry

S Ward, M Scantlebury, E Krol, P J Thomson, C Sparling, J R Speakman

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

An off-line technique is described for the preparation of H-2 from water prior to analysis of delta(2)H by dual-inlet isotope ratio mass spectrometry. H-2 is produced from sample water by reaction with LiAlH4. This provides a rapid and inexpensive method for the analysis of delta(2)H in small (10 mu L) samples of water. Precision was +/- 4.2 to 8.0 (1 sigma(n), n = 8) delta(2)H(VSMOW) for samples between 428 and 1500 delta(2)H(VSMOW), +/- 14.5 delta(2)H(VSMOW) for water enriched to 3750 delta(2)H(VSMOW) and +/- 26.0 delta(2)H(VSMOW) for water enriched to 6100 delta(2)H(VSMOW). Accuracy was +/- 1.1 to 4.2 delta(2)H(VSMOW) for water standards from natural abundance to 1000 delta(2)H(VSMOW) (the highest enrichment at which water of accepted delta(2)H is currently available). This method for delta(2)H determination is most appropriate for use with small (<50 mu L) samples of high delta(2)H enrichment such as those produced from doubly labelled water studies of small animals. The levels of measurement precision of delta(2)H would contribute 2.6-3.8% to the precision error in estimates of small animal energy expenditure made using the doubly labelled water technique when duplicate analyses are performed. Copyright (C) 2000 John Whey & Sons, Ltd.

Original languageEnglish
Pages (from-to)450-453
Number of pages4
JournalRapid Communications in Mass Spectrometry
Volume14
Publication statusPublished - 2000

Keywords

  • EQUILIBRATION TECHNIQUE
  • DELTA-O-18 MEASUREMENT
  • SAMPLES
  • H2O

Cite this

Preparation of hydrogen from water by reduction with lithium aluminium hydride for the analysis of delta H-2 by isotope ratio mass spectrometry. / Ward, S ; Scantlebury, M ; Krol, E ; Thomson, P J ; Sparling, C ; Speakman, J R .

In: Rapid Communications in Mass Spectrometry, Vol. 14, 2000, p. 450-453.

Research output: Contribution to journalArticle

@article{a64f7ee7f5b2436caf555056f9f1bed0,
title = "Preparation of hydrogen from water by reduction with lithium aluminium hydride for the analysis of delta H-2 by isotope ratio mass spectrometry",
abstract = "An off-line technique is described for the preparation of H-2 from water prior to analysis of delta(2)H by dual-inlet isotope ratio mass spectrometry. H-2 is produced from sample water by reaction with LiAlH4. This provides a rapid and inexpensive method for the analysis of delta(2)H in small (10 mu L) samples of water. Precision was +/- 4.2 to 8.0 (1 sigma(n), n = 8) delta(2)H(VSMOW) for samples between 428 and 1500 delta(2)H(VSMOW), +/- 14.5 delta(2)H(VSMOW) for water enriched to 3750 delta(2)H(VSMOW) and +/- 26.0 delta(2)H(VSMOW) for water enriched to 6100 delta(2)H(VSMOW). Accuracy was +/- 1.1 to 4.2 delta(2)H(VSMOW) for water standards from natural abundance to 1000 delta(2)H(VSMOW) (the highest enrichment at which water of accepted delta(2)H is currently available). This method for delta(2)H determination is most appropriate for use with small (<50 mu L) samples of high delta(2)H enrichment such as those produced from doubly labelled water studies of small animals. The levels of measurement precision of delta(2)H would contribute 2.6-3.8{\%} to the precision error in estimates of small animal energy expenditure made using the doubly labelled water technique when duplicate analyses are performed. Copyright (C) 2000 John Whey & Sons, Ltd.",
keywords = "EQUILIBRATION TECHNIQUE, DELTA-O-18 MEASUREMENT, SAMPLES, H2O",
author = "S Ward and M Scantlebury and E Krol and Thomson, {P J} and C Sparling and Speakman, {J R}",
year = "2000",
language = "English",
volume = "14",
pages = "450--453",
journal = "Rapid Communications in Mass Spectrometry",
issn = "0951-4198",
publisher = "John Wiley & Sons Ltd.",

}

TY - JOUR

T1 - Preparation of hydrogen from water by reduction with lithium aluminium hydride for the analysis of delta H-2 by isotope ratio mass spectrometry

AU - Ward, S

AU - Scantlebury, M

AU - Krol, E

AU - Thomson, P J

AU - Sparling, C

AU - Speakman, J R

PY - 2000

Y1 - 2000

N2 - An off-line technique is described for the preparation of H-2 from water prior to analysis of delta(2)H by dual-inlet isotope ratio mass spectrometry. H-2 is produced from sample water by reaction with LiAlH4. This provides a rapid and inexpensive method for the analysis of delta(2)H in small (10 mu L) samples of water. Precision was +/- 4.2 to 8.0 (1 sigma(n), n = 8) delta(2)H(VSMOW) for samples between 428 and 1500 delta(2)H(VSMOW), +/- 14.5 delta(2)H(VSMOW) for water enriched to 3750 delta(2)H(VSMOW) and +/- 26.0 delta(2)H(VSMOW) for water enriched to 6100 delta(2)H(VSMOW). Accuracy was +/- 1.1 to 4.2 delta(2)H(VSMOW) for water standards from natural abundance to 1000 delta(2)H(VSMOW) (the highest enrichment at which water of accepted delta(2)H is currently available). This method for delta(2)H determination is most appropriate for use with small (<50 mu L) samples of high delta(2)H enrichment such as those produced from doubly labelled water studies of small animals. The levels of measurement precision of delta(2)H would contribute 2.6-3.8% to the precision error in estimates of small animal energy expenditure made using the doubly labelled water technique when duplicate analyses are performed. Copyright (C) 2000 John Whey & Sons, Ltd.

AB - An off-line technique is described for the preparation of H-2 from water prior to analysis of delta(2)H by dual-inlet isotope ratio mass spectrometry. H-2 is produced from sample water by reaction with LiAlH4. This provides a rapid and inexpensive method for the analysis of delta(2)H in small (10 mu L) samples of water. Precision was +/- 4.2 to 8.0 (1 sigma(n), n = 8) delta(2)H(VSMOW) for samples between 428 and 1500 delta(2)H(VSMOW), +/- 14.5 delta(2)H(VSMOW) for water enriched to 3750 delta(2)H(VSMOW) and +/- 26.0 delta(2)H(VSMOW) for water enriched to 6100 delta(2)H(VSMOW). Accuracy was +/- 1.1 to 4.2 delta(2)H(VSMOW) for water standards from natural abundance to 1000 delta(2)H(VSMOW) (the highest enrichment at which water of accepted delta(2)H is currently available). This method for delta(2)H determination is most appropriate for use with small (<50 mu L) samples of high delta(2)H enrichment such as those produced from doubly labelled water studies of small animals. The levels of measurement precision of delta(2)H would contribute 2.6-3.8% to the precision error in estimates of small animal energy expenditure made using the doubly labelled water technique when duplicate analyses are performed. Copyright (C) 2000 John Whey & Sons, Ltd.

KW - EQUILIBRATION TECHNIQUE

KW - DELTA-O-18 MEASUREMENT

KW - SAMPLES

KW - H2O

M3 - Article

VL - 14

SP - 450

EP - 453

JO - Rapid Communications in Mass Spectrometry

JF - Rapid Communications in Mass Spectrometry

SN - 0951-4198

ER -