Prevalence of Antibodies to European Bat Lyssavirus Type 2 in Scottish Bats.

M. Brookes, N. Aegerter, C. Smith, M. Healy, A. Jolliffe, Susan Swift, I. J. Mackie, J. S. Pritchard, Paul Adrian Racey, P. Moore, A. R. Fooks

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We report the first seroprevalence study of the occurrence of specific antibodies to European bat lyssavirus type 2 (EBLV-2) in Daubenton's bats. Bats were captured from 19 sites across eastern and southern Scotland. Samples from 198 Daubenton's bats, 20 Natterer's bats, and 6 Pipistrelle's bats were tested for EBLV-2. Blood samples (N = 94) were subjected to a modified fluorescent antibody virus neutralization test to determine antibody titer. From 0.05% to 3.8% (95% confidence interval) of Daubenton's bats were seropositive. Antibodies to EBLV-2 were not detected in the 2 other species tested. Mouth swabs (N = 218) were obtained, and RNA was extracted for a reverse transcription-polymerase chain reaction (RTPCR). The RT-PCR included pan lyssavirus-primers (N gene) and internal PCR control primers for ribosomal RNA. EBLV-2 RNA was not detected in any of the saliva samples tested, and live virus was not detected in virus isolation tests.

Original languageEnglish
Pages (from-to)572-578
Number of pages6
JournalEmerging Infectious Diseases
Publication statusPublished - 2005


  • TYPE-2
  • PCR
  • UK

Cite this

Brookes, M., Aegerter, N., Smith, C., Healy, M., Jolliffe, A., Swift, S., Mackie, I. J., Pritchard, J. S., Racey, P. A., Moore, P., & Fooks, A. R. (2005). Prevalence of Antibodies to European Bat Lyssavirus Type 2 in Scottish Bats. Emerging Infectious Diseases, 11, 572-578.