Prevalence of Antibodies to European Bat Lyssavirus Type 2 in Scottish Bats.

M. Brookes, N. Aegerter, C. Smith, M. Healy, A. Jolliffe, Susan Swift, I. J. Mackie, J. S. Pritchard, Paul Adrian Racey, P. Moore, A. R. Fooks

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

We report the first seroprevalence study of the occurrence of specific antibodies to European bat lyssavirus type 2 (EBLV-2) in Daubenton's bats. Bats were captured from 19 sites across eastern and southern Scotland. Samples from 198 Daubenton's bats, 20 Natterer's bats, and 6 Pipistrelle's bats were tested for EBLV-2. Blood samples (N = 94) were subjected to a modified fluorescent antibody virus neutralization test to determine antibody titer. From 0.05% to 3.8% (95% confidence interval) of Daubenton's bats were seropositive. Antibodies to EBLV-2 were not detected in the 2 other species tested. Mouth swabs (N = 218) were obtained, and RNA was extracted for a reverse transcription-polymerase chain reaction (RTPCR). The RT-PCR included pan lyssavirus-primers (N gene) and internal PCR control primers for ribosomal RNA. EBLV-2 RNA was not detected in any of the saliva samples tested, and live virus was not detected in virus isolation tests.

Original languageEnglish
Pages (from-to)572-578
Number of pages6
JournalEmerging Infectious Diseases
Volume11
Publication statusPublished - 2005

Keywords

  • RABIES-RELATED VIRUSES
  • DAUBENTONS BAT
  • UNITED-KINGDOM
  • TYPE-2
  • IDENTIFICATION
  • POPULATIONS
  • INFECTION
  • PCR
  • UK

Cite this

Brookes, M., Aegerter, N., Smith, C., Healy, M., Jolliffe, A., Swift, S., ... Fooks, A. R. (2005). Prevalence of Antibodies to European Bat Lyssavirus Type 2 in Scottish Bats. Emerging Infectious Diseases, 11, 572-578.

Prevalence of Antibodies to European Bat Lyssavirus Type 2 in Scottish Bats. / Brookes, M.; Aegerter, N.; Smith, C.; Healy, M.; Jolliffe, A.; Swift, Susan; Mackie, I. J.; Pritchard, J. S.; Racey, Paul Adrian; Moore, P.; Fooks, A. R.

In: Emerging Infectious Diseases, Vol. 11, 2005, p. 572-578.

Research output: Contribution to journalArticle

Brookes, M, Aegerter, N, Smith, C, Healy, M, Jolliffe, A, Swift, S, Mackie, IJ, Pritchard, JS, Racey, PA, Moore, P & Fooks, AR 2005, 'Prevalence of Antibodies to European Bat Lyssavirus Type 2 in Scottish Bats.', Emerging Infectious Diseases, vol. 11, pp. 572-578.
Brookes M, Aegerter N, Smith C, Healy M, Jolliffe A, Swift S et al. Prevalence of Antibodies to European Bat Lyssavirus Type 2 in Scottish Bats. Emerging Infectious Diseases. 2005;11:572-578.
Brookes, M. ; Aegerter, N. ; Smith, C. ; Healy, M. ; Jolliffe, A. ; Swift, Susan ; Mackie, I. J. ; Pritchard, J. S. ; Racey, Paul Adrian ; Moore, P. ; Fooks, A. R. / Prevalence of Antibodies to European Bat Lyssavirus Type 2 in Scottish Bats. In: Emerging Infectious Diseases. 2005 ; Vol. 11. pp. 572-578.
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