Prevalence of Panton-Valentine Leukocidin (PVL) and Antimicrobial Resistance in Community-Acquired Clinical Staphylococcus aureus in an Urban Gambian Hospital: A 11-Year Period Retrospective Pilot Study

Saffiatou Darboe* (Corresponding Author), Sarah Dobreniecki, Sheikh Jarju, Mamadou Jallow, Nuredin Ibrahim Mohammed, Miriam Wathuo, Buntung Ceesay, Sam Tweed, Robindra Basu Roy, Uduak Okomo, Brenda Kwambana-Adams, Martin Antonio, Richard S Bradbury, Thushan I de Silva, Karen Forrest, Anna Roca, Bolarinde Joseph Lawal, Davis Nwakanma, Ousman Secka

*Corresponding author for this work

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background:Staphylococcus aureus is a major human pathogen. Panton-Valentine leukocidin (PVL) is a virulence factor produced by some strains that causes leukocyte lysis and tissue necrosis. PVL-associated S. aureus (PVL-SA) predominantly causes skin and soft-tissue infections (SSTIs) but can also cause invasive infections such as necrotizing pneumonia. It is carried by both community-associated methicillin susceptible S. aureus (CA-MSSA) and methicillin resistant S. aureus (CA-MRSA). This study aims to determine the prevalence of PVL-SA among patients seen at an urban Gambian hospital and associated antibiotic resistance. Methods: Archived clinical S. aureus (70 invasive bacteraemia and 223 non-invasive SSTIs) from 293 patients were retrieved as well as relevant data from clinical records where available. Antibiotic susceptibility was assessed using disc diffusion according to Clinical Laboratory Standards Institute (CLSI) guidelines. Genomic DNA was extracted and the presence of lukF and lukS PVL genes was detected by conventional gel-based PCR. Result: PVL-SA strains accounted for 61.4% (180/293) of S. aureus isolates. PVL prevalence was high in both Gambian bacteraemia and SSTIs S. aureus strains. Antimicrobial resistance was low and included chloramphenicol (4.8%), cefoxitin (2.4%), ciprofloxacin (3.8%), erythromycin (8.9%), gentamicin (5.5%) penicillin (92.5%), tetracycline (41.0%), and sulfamethoxazole-trimethoprim (24.2%). There was no association of PVL with antimicrobial resistance. Conclusion: PVL expression is high among clinical S. aureus strains among Gambian patients. Reporting of PVL-SA clinical infections is necessary to enable the monitoring of the clinical impact of these strains in the population and guide prevention of the spread of virulent PVL-positive CA-MRSA strains. SUMMARY Staphylococcus aureus (S. aureus) is a major human pathogen with several virulence factors. We performed a retrospective analysis to investigate the prevalence of one such virulence factor (PVL) amongst clinical S. aureus samples. We found a high prevalence in our setting but antimicrobial resistance including methicillin resistance was low.

Original languageEnglish
Article number170
JournalFrontiers in cellular and infection microbiology
Volume9
DOIs
Publication statusPublished - 22 May 2019

Fingerprint

Urban Hospitals
Staphylococcus aureus
Retrospective Studies
Soft Tissue Infections
Virulence Factors
Methicillin-Resistant Staphylococcus aureus
Bacteremia
Skin
Panton-Valentine leukocidin
Cefoxitin
Methicillin Resistance
Methicillin
Sulfamethoxazole Drug Combination Trimethoprim
Chloramphenicol
Erythromycin
Ciprofloxacin
Microbial Drug Resistance
Infection
Gentamicins
Tetracycline

Keywords

  • Panton-Valentine leukocidin
  • staphylococcus aureus
  • community-acquired infections
  • antimicrobial resistance
  • The Gambia
  • Community-acquired
  • Antimicrobial resistance
  • Staphylococcus aureus
  • RISK-FACTORS
  • TOXIN
  • LINEAGES
  • METHICILLIN-RESISTANT
  • SKIN INFECTIONS
  • DISEASE
  • community-acquired
  • EPIDEMIOLOGY

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases
  • Microbiology
  • Immunology

Cite this

Prevalence of Panton-Valentine Leukocidin (PVL) and Antimicrobial Resistance in Community-Acquired Clinical Staphylococcus aureus in an Urban Gambian Hospital : A 11-Year Period Retrospective Pilot Study. / Darboe, Saffiatou (Corresponding Author); Dobreniecki, Sarah; Jarju, Sheikh; Jallow, Mamadou; Mohammed, Nuredin Ibrahim; Wathuo, Miriam; Ceesay, Buntung; Tweed, Sam; Basu Roy, Robindra; Okomo, Uduak; Kwambana-Adams, Brenda; Antonio, Martin; Bradbury, Richard S; de Silva, Thushan I; Forrest, Karen; Roca, Anna; Lawal, Bolarinde Joseph; Nwakanma, Davis; Secka, Ousman.

In: Frontiers in cellular and infection microbiology, Vol. 9, 170, 22.05.2019.

Research output: Contribution to journalArticle

Darboe, S, Dobreniecki, S, Jarju, S, Jallow, M, Mohammed, NI, Wathuo, M, Ceesay, B, Tweed, S, Basu Roy, R, Okomo, U, Kwambana-Adams, B, Antonio, M, Bradbury, RS, de Silva, TI, Forrest, K, Roca, A, Lawal, BJ, Nwakanma, D & Secka, O 2019, 'Prevalence of Panton-Valentine Leukocidin (PVL) and Antimicrobial Resistance in Community-Acquired Clinical Staphylococcus aureus in an Urban Gambian Hospital: A 11-Year Period Retrospective Pilot Study', Frontiers in cellular and infection microbiology, vol. 9, 170. https://doi.org/10.3389/fcimb.2019.00170
Darboe, Saffiatou ; Dobreniecki, Sarah ; Jarju, Sheikh ; Jallow, Mamadou ; Mohammed, Nuredin Ibrahim ; Wathuo, Miriam ; Ceesay, Buntung ; Tweed, Sam ; Basu Roy, Robindra ; Okomo, Uduak ; Kwambana-Adams, Brenda ; Antonio, Martin ; Bradbury, Richard S ; de Silva, Thushan I ; Forrest, Karen ; Roca, Anna ; Lawal, Bolarinde Joseph ; Nwakanma, Davis ; Secka, Ousman. / Prevalence of Panton-Valentine Leukocidin (PVL) and Antimicrobial Resistance in Community-Acquired Clinical Staphylococcus aureus in an Urban Gambian Hospital : A 11-Year Period Retrospective Pilot Study. In: Frontiers in cellular and infection microbiology. 2019 ; Vol. 9.
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title = "Prevalence of Panton-Valentine Leukocidin (PVL) and Antimicrobial Resistance in Community-Acquired Clinical Staphylococcus aureus in an Urban Gambian Hospital: A 11-Year Period Retrospective Pilot Study",
abstract = "Background:Staphylococcus aureus is a major human pathogen. Panton-Valentine leukocidin (PVL) is a virulence factor produced by some strains that causes leukocyte lysis and tissue necrosis. PVL-associated S. aureus (PVL-SA) predominantly causes skin and soft-tissue infections (SSTIs) but can also cause invasive infections such as necrotizing pneumonia. It is carried by both community-associated methicillin susceptible S. aureus (CA-MSSA) and methicillin resistant S. aureus (CA-MRSA). This study aims to determine the prevalence of PVL-SA among patients seen at an urban Gambian hospital and associated antibiotic resistance. Methods: Archived clinical S. aureus (70 invasive bacteraemia and 223 non-invasive SSTIs) from 293 patients were retrieved as well as relevant data from clinical records where available. Antibiotic susceptibility was assessed using disc diffusion according to Clinical Laboratory Standards Institute (CLSI) guidelines. Genomic DNA was extracted and the presence of lukF and lukS PVL genes was detected by conventional gel-based PCR. Result: PVL-SA strains accounted for 61.4{\%} (180/293) of S. aureus isolates. PVL prevalence was high in both Gambian bacteraemia and SSTIs S. aureus strains. Antimicrobial resistance was low and included chloramphenicol (4.8{\%}), cefoxitin (2.4{\%}), ciprofloxacin (3.8{\%}), erythromycin (8.9{\%}), gentamicin (5.5{\%}) penicillin (92.5{\%}), tetracycline (41.0{\%}), and sulfamethoxazole-trimethoprim (24.2{\%}). There was no association of PVL with antimicrobial resistance. Conclusion: PVL expression is high among clinical S. aureus strains among Gambian patients. Reporting of PVL-SA clinical infections is necessary to enable the monitoring of the clinical impact of these strains in the population and guide prevention of the spread of virulent PVL-positive CA-MRSA strains. SUMMARY Staphylococcus aureus (S. aureus) is a major human pathogen with several virulence factors. We performed a retrospective analysis to investigate the prevalence of one such virulence factor (PVL) amongst clinical S. aureus samples. We found a high prevalence in our setting but antimicrobial resistance including methicillin resistance was low.",
keywords = "Panton-Valentine leukocidin, staphylococcus aureus, community-acquired infections, antimicrobial resistance, The Gambia, Community-acquired, Antimicrobial resistance, Staphylococcus aureus, RISK-FACTORS, TOXIN, LINEAGES, METHICILLIN-RESISTANT, SKIN INFECTIONS, DISEASE, community-acquired, EPIDEMIOLOGY",
author = "Saffiatou Darboe and Sarah Dobreniecki and Sheikh Jarju and Mamadou Jallow and Mohammed, {Nuredin Ibrahim} and Miriam Wathuo and Buntung Ceesay and Sam Tweed and {Basu Roy}, Robindra and Uduak Okomo and Brenda Kwambana-Adams and Martin Antonio and Bradbury, {Richard S} and {de Silva}, {Thushan I} and Karen Forrest and Anna Roca and Lawal, {Bolarinde Joseph} and Davis Nwakanma and Ousman Secka",
note = "This work is supported by MRCG. The source of funding has no role in the design and writing of the manuscript.",
year = "2019",
month = "5",
day = "22",
doi = "10.3389/fcimb.2019.00170",
language = "English",
volume = "9",
journal = "Frontiers in cellular and infection microbiology",
issn = "2235-2988",
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TY - JOUR

T1 - Prevalence of Panton-Valentine Leukocidin (PVL) and Antimicrobial Resistance in Community-Acquired Clinical Staphylococcus aureus in an Urban Gambian Hospital

T2 - A 11-Year Period Retrospective Pilot Study

AU - Darboe, Saffiatou

AU - Dobreniecki, Sarah

AU - Jarju, Sheikh

AU - Jallow, Mamadou

AU - Mohammed, Nuredin Ibrahim

AU - Wathuo, Miriam

AU - Ceesay, Buntung

AU - Tweed, Sam

AU - Basu Roy, Robindra

AU - Okomo, Uduak

AU - Kwambana-Adams, Brenda

AU - Antonio, Martin

AU - Bradbury, Richard S

AU - de Silva, Thushan I

AU - Forrest, Karen

AU - Roca, Anna

AU - Lawal, Bolarinde Joseph

AU - Nwakanma, Davis

AU - Secka, Ousman

N1 - This work is supported by MRCG. The source of funding has no role in the design and writing of the manuscript.

PY - 2019/5/22

Y1 - 2019/5/22

N2 - Background:Staphylococcus aureus is a major human pathogen. Panton-Valentine leukocidin (PVL) is a virulence factor produced by some strains that causes leukocyte lysis and tissue necrosis. PVL-associated S. aureus (PVL-SA) predominantly causes skin and soft-tissue infections (SSTIs) but can also cause invasive infections such as necrotizing pneumonia. It is carried by both community-associated methicillin susceptible S. aureus (CA-MSSA) and methicillin resistant S. aureus (CA-MRSA). This study aims to determine the prevalence of PVL-SA among patients seen at an urban Gambian hospital and associated antibiotic resistance. Methods: Archived clinical S. aureus (70 invasive bacteraemia and 223 non-invasive SSTIs) from 293 patients were retrieved as well as relevant data from clinical records where available. Antibiotic susceptibility was assessed using disc diffusion according to Clinical Laboratory Standards Institute (CLSI) guidelines. Genomic DNA was extracted and the presence of lukF and lukS PVL genes was detected by conventional gel-based PCR. Result: PVL-SA strains accounted for 61.4% (180/293) of S. aureus isolates. PVL prevalence was high in both Gambian bacteraemia and SSTIs S. aureus strains. Antimicrobial resistance was low and included chloramphenicol (4.8%), cefoxitin (2.4%), ciprofloxacin (3.8%), erythromycin (8.9%), gentamicin (5.5%) penicillin (92.5%), tetracycline (41.0%), and sulfamethoxazole-trimethoprim (24.2%). There was no association of PVL with antimicrobial resistance. Conclusion: PVL expression is high among clinical S. aureus strains among Gambian patients. Reporting of PVL-SA clinical infections is necessary to enable the monitoring of the clinical impact of these strains in the population and guide prevention of the spread of virulent PVL-positive CA-MRSA strains. SUMMARY Staphylococcus aureus (S. aureus) is a major human pathogen with several virulence factors. We performed a retrospective analysis to investigate the prevalence of one such virulence factor (PVL) amongst clinical S. aureus samples. We found a high prevalence in our setting but antimicrobial resistance including methicillin resistance was low.

AB - Background:Staphylococcus aureus is a major human pathogen. Panton-Valentine leukocidin (PVL) is a virulence factor produced by some strains that causes leukocyte lysis and tissue necrosis. PVL-associated S. aureus (PVL-SA) predominantly causes skin and soft-tissue infections (SSTIs) but can also cause invasive infections such as necrotizing pneumonia. It is carried by both community-associated methicillin susceptible S. aureus (CA-MSSA) and methicillin resistant S. aureus (CA-MRSA). This study aims to determine the prevalence of PVL-SA among patients seen at an urban Gambian hospital and associated antibiotic resistance. Methods: Archived clinical S. aureus (70 invasive bacteraemia and 223 non-invasive SSTIs) from 293 patients were retrieved as well as relevant data from clinical records where available. Antibiotic susceptibility was assessed using disc diffusion according to Clinical Laboratory Standards Institute (CLSI) guidelines. Genomic DNA was extracted and the presence of lukF and lukS PVL genes was detected by conventional gel-based PCR. Result: PVL-SA strains accounted for 61.4% (180/293) of S. aureus isolates. PVL prevalence was high in both Gambian bacteraemia and SSTIs S. aureus strains. Antimicrobial resistance was low and included chloramphenicol (4.8%), cefoxitin (2.4%), ciprofloxacin (3.8%), erythromycin (8.9%), gentamicin (5.5%) penicillin (92.5%), tetracycline (41.0%), and sulfamethoxazole-trimethoprim (24.2%). There was no association of PVL with antimicrobial resistance. Conclusion: PVL expression is high among clinical S. aureus strains among Gambian patients. Reporting of PVL-SA clinical infections is necessary to enable the monitoring of the clinical impact of these strains in the population and guide prevention of the spread of virulent PVL-positive CA-MRSA strains. SUMMARY Staphylococcus aureus (S. aureus) is a major human pathogen with several virulence factors. We performed a retrospective analysis to investigate the prevalence of one such virulence factor (PVL) amongst clinical S. aureus samples. We found a high prevalence in our setting but antimicrobial resistance including methicillin resistance was low.

KW - Panton-Valentine leukocidin

KW - staphylococcus aureus

KW - community-acquired infections

KW - antimicrobial resistance

KW - The Gambia

KW - Community-acquired

KW - Antimicrobial resistance

KW - Staphylococcus aureus

KW - RISK-FACTORS

KW - TOXIN

KW - LINEAGES

KW - METHICILLIN-RESISTANT

KW - SKIN INFECTIONS

KW - DISEASE

KW - community-acquired

KW - EPIDEMIOLOGY

UR - http://www.scopus.com/inward/record.url?scp=85068168747&partnerID=8YFLogxK

U2 - 10.3389/fcimb.2019.00170

DO - 10.3389/fcimb.2019.00170

M3 - Article

C2 - 31192162

VL - 9

JO - Frontiers in cellular and infection microbiology

JF - Frontiers in cellular and infection microbiology

SN - 2235-2988

M1 - 170

ER -