PriA helicase and SSB interact physically and functionally

Chris James Cadman, Peter McGlynn

Research output: Contribution to journalArticle

114 Citations (Scopus)

Abstract

PriA helicase is the major DNA replication restart initiator in Escherichia coli and acts to reload the replicative helicase DnaB back onto the chromosome at repaired replication forks and D-loops formed by recombination. We have discovered that PriA-catalysed unwinding of branched DNA substrates is stimulated specifically by contact with the single-strand DNA binding protein of E.coli, SSB. This stimulation requires binding of SSB to the initial DNA substrate and is effected via a physical interaction between PriA and the C-terminus of SSB. Stimulation of PriA by the SSB C-terminus may act to ensure that efficient PriA-catalysed reloading of DnaB occurs only onto the lagging strand template of repaired forks and D-loops. Correlation between the DNA repair and recombination defects of strains harbouring an SSB C-terminal mutation with inhibition of this SSB-PriA interaction in vitro suggests that SSB plays a critical role in facilitating PriA-directed replication restart. Taken together with previous data, these findings indicate that protein-protein interactions involving SSB may coordinate replication fork reloading from start to finish.

Original languageEnglish
Pages (from-to)6378-6387
Number of pages9
JournalNucleic Acids Research
Volume32
Issue number21
DOIs
Publication statusPublished - Dec 2004

Keywords

  • SINGLE-STRANDED-DNA
  • PHI X174-TYPE PRIMOSOME
  • ESCHERICHIA-COLI K-12
  • BINDING-PROTEIN
  • REPLICATION FORK
  • SULFOLOBUS-SOLFATARICUS
  • RECOMBINATIONAL REPAIR
  • LAGGING-STRAND
  • GENETIC-RECOMBINATION
  • CHI SUBUNIT

Cite this