Primürstruktur der Adipokinetischen Hormone II von Heuschrecken

Karl Siegert; Peter Morgan; William Mordue

doi:10.1515/bchm3.1985.366.2.723

Primürstruktur der Adipokinetischen Hormone II von Heuschrecken

Translated title of the contribution: Primary Structures of Locust Adipokinetic Hormones II

Karl Siegert, Peter Morgan, William Mordue

Research output: Contribution to journal › Article › peer-review

127 Citations (Scopus)

Abstract

Adipokinetic hormones II from corpora cardiaca of the locusts Schistocerca gregaria and Locusta migratoria, respectively, have been isolated and their primary structures elucidated. Both octapeptides are N-terminally blocked by a 5-oxoproline (pyroglutamate) residue and had to be cleaved by 5-oxoprolyl-peptidase to make them available for the Edman degradation method carried out with a gas-phase sequencer. The C- termini are blocked as both peptides are not cleaved by carboxypeptidase A; the presence of C-terminal amide groups is very likely. AKHII (S. gregaria) Glu-Leu-Asn-Phe-Ser-Thr-Gly-Trp-NHi AKHII (L. migratoria) Glu-Leu-Asn-Phe-Ser-Ala-Gly-Trp-NH2.

Translated title of the contribution	Primary Structures of Locust Adipokinetic Hormones II
Original language	Other
Pages (from-to)	723-728
Number of pages	6
Journal	Biological Chemistry Hoppe-Seyler
Volume	366
Issue number	2
DOIs	https://doi.org/10.1515/bchm3.1985.366.2.723
Publication status	Published - 1 Jan 1985

Keywords

AKHII
gas-phase sequencing
Locusts
primary structure
reversed-phase high-performance liquid chromatography

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10.1515/bchm3.1985.366.2.723

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title = "Prim{\"u}rstruktur der Adipokinetischen Hormone II von Heuschrecken",

abstract = "Adipokinetic hormones II from corpora cardiaca of the locusts Schistocerca gregaria and Locusta migratoria, respectively, have been isolated and their primary structures elucidated. Both octapeptides are N-terminally blocked by a 5-oxoproline (pyroglutamate) residue and had to be cleaved by 5-oxoprolyl-peptidase to make them available for the Edman degradation method carried out with a gas-phase sequencer. The C- termini are blocked as both peptides are not cleaved by carboxypeptidase A; the presence of C-terminal amide groups is very likely. AKHII (S. gregaria) Glu-Leu-Asn-Phe-Ser-Thr-Gly-Trp-NHi AKHII (L. migratoria) Glu-Leu-Asn-Phe-Ser-Ala-Gly-Trp-NH2.",

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Y1 - 1985/1/1

N2 - Adipokinetic hormones II from corpora cardiaca of the locusts Schistocerca gregaria and Locusta migratoria, respectively, have been isolated and their primary structures elucidated. Both octapeptides are N-terminally blocked by a 5-oxoproline (pyroglutamate) residue and had to be cleaved by 5-oxoprolyl-peptidase to make them available for the Edman degradation method carried out with a gas-phase sequencer. The C- termini are blocked as both peptides are not cleaved by carboxypeptidase A; the presence of C-terminal amide groups is very likely. AKHII (S. gregaria) Glu-Leu-Asn-Phe-Ser-Thr-Gly-Trp-NHi AKHII (L. migratoria) Glu-Leu-Asn-Phe-Ser-Ala-Gly-Trp-NH2.

AB - Adipokinetic hormones II from corpora cardiaca of the locusts Schistocerca gregaria and Locusta migratoria, respectively, have been isolated and their primary structures elucidated. Both octapeptides are N-terminally blocked by a 5-oxoproline (pyroglutamate) residue and had to be cleaved by 5-oxoprolyl-peptidase to make them available for the Edman degradation method carried out with a gas-phase sequencer. The C- termini are blocked as both peptides are not cleaved by carboxypeptidase A; the presence of C-terminal amide groups is very likely. AKHII (S. gregaria) Glu-Leu-Asn-Phe-Ser-Thr-Gly-Trp-NHi AKHII (L. migratoria) Glu-Leu-Asn-Phe-Ser-Ala-Gly-Trp-NH2.

KW - AKHII

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KW - Locusts

KW - primary structure

KW - reversed-phase high-performance liquid chromatography

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ER -

Primürstruktur der Adipokinetischen Hormone II von Heuschrecken

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Keywords

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