Abstract
Adipokinetic hormones II from corpora cardiaca of the locusts Schistocerca gregaria and Locusta migratoria, respectively, have been isolated and their primary structures elucidated. Both octapeptides are N-terminally blocked by a 5-oxoproline (pyroglutamate) residue and had to be cleaved by 5-oxoprolyl-peptidase to make them available for the Edman degradation method carried out with a gas-phase sequencer. The C- termini are blocked as both peptides are not cleaved by carboxypeptidase A; the presence of C-terminal amide groups is very likely. AKHII (S. gregaria) Glu-Leu-Asn-Phe-Ser-Thr-Gly-Trp-NHi AKHII (L. migratoria) Glu-Leu-Asn-Phe-Ser-Ala-Gly-Trp-NH2.
Translated title of the contribution | Primary Structures of Locust Adipokinetic Hormones II |
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Original language | Other |
Pages (from-to) | 723-728 |
Number of pages | 6 |
Journal | Biological Chemistry Hoppe-Seyler |
Volume | 366 |
Issue number | 2 |
DOIs | |
Publication status | Published - 1 Jan 1985 |
Keywords
- AKHII
- gas-phase sequencing
- Locusts
- primary structure
- reversed-phase high-performance liquid chromatography