Procedures to evaluate the importance of dietary polyamines

Paul Acheampong; Mary J Macleod; Heather M Wallace

doi:10.1007/978-1-61779-034-8_22

Procedures to evaluate the importance of dietary polyamines

Paul Acheampong, Mary J Macleod, Heather M Wallace

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Polyamines not only play vital physiological functions including modulating transcription and translation of genetic material, cell proliferation and growth, ion channel regulation and cell signaling, but have also been cited in the pathogenesis of diseases. Many plant and animal sources used as food contain high amounts of polyamines. Knowledge of the content of polyamines in food as a source of these growth factors is therefore critical. A 2-step perchloric acid precipitation method to obtain acid soluble extracts from food that are subsequently taken through a dansylation process to produce dansyl polyamine derivatives for HPLC measurement is described. Examples are provided to illustrate mathematical correction factors.

Original language	English
Pages (from-to)	349-364
Number of pages	16
Journal	Methods in Molecular Medicine
Volume	720
Issue number	6
DOIs	https://doi.org/10.1007/978-1-61779-034-8_22
Publication status	Published - 1 Jan 2011

Keywords

Acetonitriles
Animals
Chemical Precipitation
Chromatography, High Pressure Liquid
Complex Mixtures
Dansyl Compounds
Diet
Food Analysis
Perchloric Acid
Polyamines
Putrescine
Reference Standards
Solubility
Solutions
Spermidine
Spermine
Toluene

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title = "Procedures to evaluate the importance of dietary polyamines",

abstract = "Polyamines not only play vital physiological functions including modulating transcription and translation of genetic material, cell proliferation and growth, ion channel regulation and cell signaling, but have also been cited in the pathogenesis of diseases. Many plant and animal sources used as food contain high amounts of polyamines. Knowledge of the content of polyamines in food as a source of these growth factors is therefore critical. A 2-step perchloric acid precipitation method to obtain acid soluble extracts from food that are subsequently taken through a dansylation process to produce dansyl polyamine derivatives for HPLC measurement is described. Examples are provided to illustrate mathematical correction factors.",

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T1 - Procedures to evaluate the importance of dietary polyamines

AU - Acheampong, Paul

AU - Macleod, Mary J

AU - Wallace, Heather M

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N2 - Polyamines not only play vital physiological functions including modulating transcription and translation of genetic material, cell proliferation and growth, ion channel regulation and cell signaling, but have also been cited in the pathogenesis of diseases. Many plant and animal sources used as food contain high amounts of polyamines. Knowledge of the content of polyamines in food as a source of these growth factors is therefore critical. A 2-step perchloric acid precipitation method to obtain acid soluble extracts from food that are subsequently taken through a dansylation process to produce dansyl polyamine derivatives for HPLC measurement is described. Examples are provided to illustrate mathematical correction factors.

AB - Polyamines not only play vital physiological functions including modulating transcription and translation of genetic material, cell proliferation and growth, ion channel regulation and cell signaling, but have also been cited in the pathogenesis of diseases. Many plant and animal sources used as food contain high amounts of polyamines. Knowledge of the content of polyamines in food as a source of these growth factors is therefore critical. A 2-step perchloric acid precipitation method to obtain acid soluble extracts from food that are subsequently taken through a dansylation process to produce dansyl polyamine derivatives for HPLC measurement is described. Examples are provided to illustrate mathematical correction factors.

KW - Acetonitriles

KW - Animals

KW - Chemical Precipitation

KW - Chromatography, High Pressure Liquid

KW - Complex Mixtures

KW - Dansyl Compounds

KW - Diet

KW - Food Analysis

KW - Perchloric Acid

KW - Polyamines

KW - Putrescine

KW - Reference Standards

KW - Solubility

KW - Solutions

KW - Spermidine

KW - Spermine

KW - Toluene

U2 - 10.1007/978-1-61779-034-8_22

DO - 10.1007/978-1-61779-034-8_22

M3 - Article

C2 - 21318885

SN - 1543-1894

VL - 720

SP - 349

EP - 364

JO - Methods in Molecular Medicine

JF - Methods in Molecular Medicine

IS - 6

ER -

Procedures to evaluate the importance of dietary polyamines

Abstract

Keywords

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