Procedures to evaluate the importance of dietary polyamines

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Polyamines not only play vital physiological functions including modulating transcription and translation of genetic material, cell proliferation and growth, ion channel regulation and cell signaling, but have also been cited in the pathogenesis of diseases. Many plant and animal sources used as food contain high amounts of polyamines. Knowledge of the content of polyamines in food as a source of these growth factors is therefore critical. A 2-step perchloric acid precipitation method to obtain acid soluble extracts from food that are subsequently taken through a dansylation process to produce dansyl polyamine derivatives for HPLC measurement is described. Examples are provided to illustrate mathematical correction factors.
Original languageEnglish
Pages (from-to)349-364
Number of pages16
JournalMethods in Molecular Medicine
Volume720
Issue number6
DOIs
Publication statusPublished - 2011

Fingerprint

Polyamines
Food
Ion Channels
Intercellular Signaling Peptides and Proteins
High Pressure Liquid Chromatography
Cell Proliferation
Acids
Growth
Genes

Keywords

  • Acetonitriles
  • Animals
  • Chemical Precipitation
  • Chromatography, High Pressure Liquid
  • Complex Mixtures
  • Dansyl Compounds
  • Diet
  • Food Analysis
  • Perchloric Acid
  • Polyamines
  • Putrescine
  • Reference Standards
  • Solubility
  • Solutions
  • Spermidine
  • Spermine
  • Toluene

Cite this

Procedures to evaluate the importance of dietary polyamines. / Acheampong, Paul; Macleod, Mary J; Wallace, Heather M.

In: Methods in Molecular Medicine, Vol. 720, No. 6, 2011, p. 349-364.

Research output: Contribution to journalArticle

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