Procedures to evaluate the importance of dietary polyamines

Paul Acheampong, Mary J Macleod, Heather M Wallace

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Polyamines not only play vital physiological functions including modulating transcription and translation of genetic material, cell proliferation and growth, ion channel regulation and cell signaling, but have also been cited in the pathogenesis of diseases. Many plant and animal sources used as food contain high amounts of polyamines. Knowledge of the content of polyamines in food as a source of these growth factors is therefore critical. A 2-step perchloric acid precipitation method to obtain acid soluble extracts from food that are subsequently taken through a dansylation process to produce dansyl polyamine derivatives for HPLC measurement is described. Examples are provided to illustrate mathematical correction factors.
Original languageEnglish
Pages (from-to)349-364
Number of pages16
JournalMethods in Molecular Medicine
Issue number6
Publication statusPublished - 2011


  • Acetonitriles
  • Animals
  • Chemical Precipitation
  • Chromatography, High Pressure Liquid
  • Complex Mixtures
  • Dansyl Compounds
  • Diet
  • Food Analysis
  • Perchloric Acid
  • Polyamines
  • Putrescine
  • Reference Standards
  • Solubility
  • Solutions
  • Spermidine
  • Spermine
  • Toluene


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