Production of a functional single chain antibody attached to the surface of a plant virus

L  Smolenska; I M  Roberts; D  Learmonth; A J  Porter; W J  Harris; T M A  Wilson; S S  Cruz

Production of a functional single chain antibody attached to the surface of a plant virus

L Smolenska, I M Roberts, D Learmonth, A J Porter, W J Harris, T M A Wilson, S S Cruz

Medical Sciences

Research output: Contribution to journal › Article

52 Citations (Scopus)

Abstract

A potato virus X (PVX) vector was used to express a single chain antibody fragment (scFv) against the herbicide diuron, as a fusion to the viral coat protein. The modified virus accumulated in inoculated Nicotiana clevelandii plants and assembled to give virus particles carrying the antibody fragment. Electron microscopy was used to show that virus particles from infected leaf sap were specifically trapped on grids coated with a diuron-BSA conjugate, The results demonstrate that the PVX vector can be used as a presentation system for functional scFv. (C) 1998 Federation of European Biochemical Societies.

Original language	English
Pages (from-to)	379-382
Number of pages	4
Journal	FEBS Letters
Volume	441
Publication status	Published - 1998

Keywords

single chain antibody fragment
virus particle
potato virus X
TOBACCO MOSAIC-VIRUS
TRANSGENIC PLANTS
FOREIGN PEPTIDES
EXPRESSION
POLYPROTEIN
PROTEIN
GENE
CLEAVAGE
PARTICLE
VECTOR

Cite this

@article{e896ee1801f84a00b8b8206bd5ea288c,

title = "Production of a functional single chain antibody attached to the surface of a plant virus",

abstract = "A potato virus X (PVX) vector was used to express a single chain antibody fragment (scFv) against the herbicide diuron, as a fusion to the viral coat protein. The modified virus accumulated in inoculated Nicotiana clevelandii plants and assembled to give virus particles carrying the antibody fragment. Electron microscopy was used to show that virus particles from infected leaf sap were specifically trapped on grids coated with a diuron-BSA conjugate, The results demonstrate that the PVX vector can be used as a presentation system for functional scFv. (C) 1998 Federation of European Biochemical Societies.",

keywords = "single chain antibody fragment, virus particle, potato virus X, TOBACCO MOSAIC-VIRUS, TRANSGENIC PLANTS, FOREIGN PEPTIDES, EXPRESSION, POLYPROTEIN, PROTEIN, GENE, CLEAVAGE, PARTICLE, VECTOR",

author = "L Smolenska and Roberts, {I M} and D Learmonth and Porter, {A J} and Harris, {W J} and Wilson, {T M A} and Cruz, {S S}",

year = "1998",

language = "English",

volume = "441",

pages = "379--382",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Wiley",

}

TY - JOUR

T1 - Production of a functional single chain antibody attached to the surface of a plant virus

AU - Smolenska, L

AU - Roberts, I M

AU - Learmonth, D

AU - Porter, A J

AU - Harris, W J

AU - Wilson, T M A

AU - Cruz, S S

PY - 1998

Y1 - 1998

N2 - A potato virus X (PVX) vector was used to express a single chain antibody fragment (scFv) against the herbicide diuron, as a fusion to the viral coat protein. The modified virus accumulated in inoculated Nicotiana clevelandii plants and assembled to give virus particles carrying the antibody fragment. Electron microscopy was used to show that virus particles from infected leaf sap were specifically trapped on grids coated with a diuron-BSA conjugate, The results demonstrate that the PVX vector can be used as a presentation system for functional scFv. (C) 1998 Federation of European Biochemical Societies.

AB - A potato virus X (PVX) vector was used to express a single chain antibody fragment (scFv) against the herbicide diuron, as a fusion to the viral coat protein. The modified virus accumulated in inoculated Nicotiana clevelandii plants and assembled to give virus particles carrying the antibody fragment. Electron microscopy was used to show that virus particles from infected leaf sap were specifically trapped on grids coated with a diuron-BSA conjugate, The results demonstrate that the PVX vector can be used as a presentation system for functional scFv. (C) 1998 Federation of European Biochemical Societies.

KW - single chain antibody fragment

KW - virus particle

KW - potato virus X

KW - TOBACCO MOSAIC-VIRUS

KW - TRANSGENIC PLANTS

KW - FOREIGN PEPTIDES

KW - EXPRESSION

KW - POLYPROTEIN

KW - PROTEIN

KW - GENE

KW - CLEAVAGE

KW - PARTICLE

KW - VECTOR

M3 - Article

SN - 0014-5793

VL - 441

SP - 379

EP - 382

JO - FEBS Letters

JF - FEBS Letters

ER -

Production of a functional single chain antibody attached to the surface of a plant virus

Abstract

Keywords

Fingerprint

Cite this