Profiling excretory/secretory proteins of Trichinella spiralis muscle larvae by two-dimensional gel electrophoresis and mass spectrometry

M W Robinson, D C Gare, B Connolly

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Infection of mammalian skeletal muscle with the intracellular parasite Trichinella spiralis results in profound alterations in the host cell and a realignment of host cell gene expression. The role of parasite excretory/secretory (E/S) products in mediating these effects is unknown, largely due to the difficulty in identifying and assigning function to individual proteins. In this study, we have used two-dimensional electrophoresis to analyse the profile of muscle larva excreted/secreted proteins and have coupled this to protein identification using MALDI-TOF mass spectrometry. Interpretation of the peptide mass fingerprint data has relied primarily on the interrogation of a custom-made Trichinella EST database and the NemaGene cluster database for T spiralis. Our results suggest that this proteomic approach is a useful tool to study protein expression in Trichinella spp. and will contribute to the identification of excreted/secreted proteins. (c) 2005 Elsevier B.V. All rights reserved.

Original languageEnglish
Pages (from-to)37-41
Number of pages5
JournalVeterinary Parasitology
Volume132
Issue number1-2
Early online date29 Jun 2005
DOIs
Publication statusPublished - Sep 2005

Keywords

  • T spiralis
  • muscle larva
  • proteomics
  • maldi-tof
  • parasitic nematode
  • expression
  • products
  • cells
  • identification
  • secretion
  • sequences
  • antigens
  • cloning
  • kinase

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