Abstract
Infection of mammalian skeletal muscle with the intracellular parasite Trichinella spiralis results in profound alterations in the host cell and a realignment of host cell gene expression. The role of parasite excretory/secretory (E/S) products in mediating these effects is unknown, largely due to the difficulty in identifying and assigning function to individual proteins. In this study, we have used two-dimensional electrophoresis to analyse the profile of muscle larva excreted/secreted proteins and have coupled this to protein identification using MALDI-TOF mass spectrometry. Interpretation of the peptide mass fingerprint data has relied primarily on the interrogation of a custom-made Trichinella EST database and the NemaGene cluster database for T spiralis. Our results suggest that this proteomic approach is a useful tool to study protein expression in Trichinella spp. and will contribute to the identification of excreted/secreted proteins. (c) 2005 Elsevier B.V. All rights reserved.
| Original language | English |
|---|---|
| Pages (from-to) | 37-41 |
| Number of pages | 5 |
| Journal | Veterinary Parasitology |
| Volume | 132 |
| Issue number | 1-2 |
| Early online date | 29 Jun 2005 |
| DOIs | |
| Publication status | Published - Sep 2005 |
Keywords
- T spiralis
- muscle larva
- proteomics
- maldi-tof
- parasitic nematode
- expression
- products
- cells
- identification
- secretion
- sequences
- antigens
- cloning
- kinase
Cite this
Profiling excretory/secretory proteins of Trichinella spiralis muscle larvae by two-dimensional gel electrophoresis and mass spectrometry. / Robinson, M W ; Gare, D C ; Connolly, B .
In: Veterinary Parasitology, Vol. 132, No. 1-2, 09.2005, p. 37-41.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Profiling excretory/secretory proteins of Trichinella spiralis muscle larvae by two-dimensional gel electrophoresis and mass spectrometry
AU - Robinson, M W
AU - Gare, D C
AU - Connolly, B
PY - 2005/9
Y1 - 2005/9
N2 - Infection of mammalian skeletal muscle with the intracellular parasite Trichinella spiralis results in profound alterations in the host cell and a realignment of host cell gene expression. The role of parasite excretory/secretory (E/S) products in mediating these effects is unknown, largely due to the difficulty in identifying and assigning function to individual proteins. In this study, we have used two-dimensional electrophoresis to analyse the profile of muscle larva excreted/secreted proteins and have coupled this to protein identification using MALDI-TOF mass spectrometry. Interpretation of the peptide mass fingerprint data has relied primarily on the interrogation of a custom-made Trichinella EST database and the NemaGene cluster database for T spiralis. Our results suggest that this proteomic approach is a useful tool to study protein expression in Trichinella spp. and will contribute to the identification of excreted/secreted proteins. (c) 2005 Elsevier B.V. All rights reserved.
AB - Infection of mammalian skeletal muscle with the intracellular parasite Trichinella spiralis results in profound alterations in the host cell and a realignment of host cell gene expression. The role of parasite excretory/secretory (E/S) products in mediating these effects is unknown, largely due to the difficulty in identifying and assigning function to individual proteins. In this study, we have used two-dimensional electrophoresis to analyse the profile of muscle larva excreted/secreted proteins and have coupled this to protein identification using MALDI-TOF mass spectrometry. Interpretation of the peptide mass fingerprint data has relied primarily on the interrogation of a custom-made Trichinella EST database and the NemaGene cluster database for T spiralis. Our results suggest that this proteomic approach is a useful tool to study protein expression in Trichinella spp. and will contribute to the identification of excreted/secreted proteins. (c) 2005 Elsevier B.V. All rights reserved.
KW - T spiralis
KW - muscle larva
KW - proteomics
KW - maldi-tof
KW - parasitic nematode
KW - expression
KW - products
KW - cells
KW - identification
KW - secretion
KW - sequences
KW - antigens
KW - cloning
KW - kinase
U2 - 10.1016/j.vetpar.2005.05.019
DO - 10.1016/j.vetpar.2005.05.019
M3 - Article
VL - 132
SP - 37
EP - 41
JO - Veterinary Parasitology
JF - Veterinary Parasitology
SN - 0304-4017
IS - 1-2
ER -